Mycobacterium phage Blister
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| Detailed Information for Phage Blister | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Domnique Thayer |
| Year Found | 2012 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 36.9855 N, 86.4509 W Map |
| Discovery Notes | The phage was extracted from soil that was located directly next to a green house. Site was surrounded by buildings and very few trees. Seasonally rained on. Most likely a high amount of foot traffic at the site area. Surrounding environment consisted of weeds, some grass, plants in pots and different kinds of soil. At the time of discovery, the soil was firm, but slightly moist. |
| Naming Notes | When performing electron microscopy on this phage it was near impossible to find one phage that was intact. Every head had been either bashed in and busted open. This phage kind of reminded me as a blister. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques produced by this phage are very clear, with a size of 1-3 mm in diameter. Around the 48 hour incubation mark, halos form. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.7*10^10 |
| Date of SEA Lysate Titering | Oct 30, 2012 |
| Pitt Freezer Box# | 43 |
| Pitt Freezer Box Grid# | 2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
