These are the Pecaan Notes for Austin Powers.
There are a few genes that the BLAST would not update after a start was changed, but we have checked the results.

CDS 68 - 517
  /gene="1"
  /product="gp1"
  /function="hypothetical protein"
  /locus tag="AustinPowers_1"
    /note=Original Glimmer call @bp 68 has strength 8.25; Genemark calls start at 68
    /note=SSC: 68-517 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CHRIDISON_1 [Arthrobacter phage Chridison]],,NCBI, q1:s1 99.3289% 1.49342E-102 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.234, -2.0162541296952132, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CHRIDISON_1 [Arthrobacter phage Chridison]],,WAB09054,99.3243,1.49342E-102 SIF-HHPRED:  SIF-Syn: NKF, In same position as phage Supakev_1, downstream is terminase, large subunit

CDS 600 - 1994
  /gene="2"
  /product="gp2"
  /function="terminase, large subunit"
  /locus tag="AustinPowers_2"
    /note=Original Glimmer call @bp 600 has strength 13.0; Genemark calls start at 600
    /note=SSC: 600-1994 CP: yes SCS: both ST: SS BLAST-Start: [terminase [Arthrobacter phage Chridison]],,NCBI, q1:s58 100.0% 0.0 GAP: 82 bp gap LO: no RBS: Kibler 6, Karlin Medium, 0.922, -6.907996911792335, no F: terminase, large subunit SIF-BLAST: ,,[terminase [Arthrobacter phage Chridison]],,WAB09055,88.8676,0.0 SIF-HHPRED: Terminase large subunit; genome packaging, bacteriophage, ATPase, nuclease, VIRAL PROTEIN; HET: BR; 2.2A {Enterobacteria phage HK97},,,6Z6D_A,96.1207,100.0 SIF-Syn: terminase, large subunit, In same position as phage Supakev_2, upstream gene is NKF, downstream is portal protein

CDS 2013 - 4040
  /gene="3"
  /product="gp3"
  /function="portal protein"
  /locus tag="AustinPowers_3"
    /note=Original Glimmer call @bp 2013 has strength 12.23; Genemark calls start at 2013
    /note=SSC: 2013-4040 CP: yes SCS: both ST: SS BLAST-Start: [portal protein [Arthrobacter phage Chridison]],,NCBI, q1:s1 100.0% 0.0 GAP: 18 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.632, -3.595227487224058, yes F: portal protein SIF-BLAST: ,,[portal protein [Arthrobacter phage Chridison]],,WAB09056,99.8519,0.0 SIF-HHPRED: Portal protein; Prohead I, icosahedral symmetry, HK97, phage, capsid, VIRUS; 3.6A {Escherichia phage HK97},,,8FQL_D,54.8148,100.0 SIF-Syn: portal protein, in same position as Supakev_3, upstream is terminase, large subunit, downstream is capsid maturation protease

CDS 4059 - 4835
  /gene="4"
  /product="gp4"
  /function="capsid maturation protease"
  /locus tag="AustinPowers_4"
    /note=Original Glimmer call @bp 4059 has strength 14.14; Genemark calls start at 4065
    /note=SSC: 4059-4835 CP: yes SCS: both-gl ST: NI BLAST-Start: [capsid maturation protease [Arthrobacter phage Chridison]],,NCBI, q1:s1 100.0% 0.0 GAP: 18 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.603, -3.368377069717189, yes F: capsid maturation protease SIF-BLAST: ,,[capsid maturation protease [Arthrobacter phage Chridison]],,WAB09057,99.6124,0.0 SIF-HHPRED: Peptidase_S78 ; Caudovirus prohead serine protease,,,PF04586.20,64.3411,99.7 SIF-Syn: capsid maturation protease, in same position as Supakev_4, upstream is , downstream is major capsid protein

CDS 4835 - 6082
  /gene="5"
  /product="gp5"
  /function="major capsid protein"
  /locus tag="AustinPowers_5"
    /note=Original Glimmer call @bp 4835 has strength 17.23; Genemark calls start at 4835
    /note=SSC: 4835-6082 CP: yes SCS: both ST: SS BLAST-Start: [major capsid protein [Arthrobacter phage Chridison]],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.006, -3.095100142625534, yes F: major capsid protein SIF-BLAST: ,,[major capsid protein [Arthrobacter phage Chridison]],,WAB09058,100.0,0.0 SIF-HHPRED: Scaffolding domain delta; Prohead I, icosahedral symmetry, HK97, phage, capsid, VIRUS; 3.5A {Escherichia phage HK97},,,8FQK_F,99.5181,100.0 SIF-Syn: major capsid protein, in same position as Supakev_5, upstream is capsid maturation protease, downstream is NKF

CDS 6160 - 6333
  /gene="6"
  /product="gp6"
  /function="hypothetical protein"
  /locus tag="AustinPowers_6"
    /note=Original Glimmer call @bp 6160 has strength 14.65; Genemark calls start at 6160
    /note=SSC: 6160-6333 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_CHRIDISON_6 [Arthrobacter phage Chridison]],,NCBI, q1:s1 100.0% 1.04396E-30 GAP: 77 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.713, -3.2123487306667524, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CHRIDISON_6 [Arthrobacter phage Chridison]],,WAB09059,98.2456,1.04396E-30 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_6, upstream is major capsid protein, downstream is head-to-tail adaptor

CDS 6343 - 6924
  /gene="7"
  /product="gp7"
  /function="head-to-tail adaptor"
  /locus tag="AustinPowers_7"
    /note=Original Glimmer call @bp 6442 has strength 6.71; Genemark calls start at 6343
    /note=SSC: 6343-6924 CP: yes SCS: both-gm ST: NI BLAST-Start: [head-tail adaptor [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 2.85118E-136 GAP: 9 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.889, -5.186767100221219, no F: head-to-tail adaptor SIF-BLAST: ,,[head-tail adaptor [Arthrobacter phage HunterDalle] ],,YP_009602627,100.0,2.85118E-136 SIF-HHPRED: Head completion protein; Neck, Portal, T5, VIRUS, VIRAL PROTEIN; 3.2A {Escherichia phage DT57C},,,8HQO_R,93.2643,99.9 SIF-Syn: head-to-tail adaptor, in same position as Supakev_7, upstream is NKF, downstream is head-to-tail stopper

CDS 6924 - 7262
  /gene="8"
  /product="gp8"
  /function="head-to-tail stopper"
  /locus tag="AustinPowers_8"
    /note=Original Glimmer call @bp 6924 has strength 8.93; Genemark calls start at 6924
    /note=SSC: 6924-7262 CP: yes SCS: both ST: SS BLAST-Start: [head closure Hc1 [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 3.07084E-77 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.097, -4.532304881730868, no F: head-to-tail stopper SIF-BLAST: ,,[head closure Hc1 [Arthrobacter phage HunterDalle] ],,YP_009602628,100.0,3.07084E-77 SIF-HHPRED: Phage-like element PBSX protein xkdH; NESG X-Ray SR362 P54328 Structure, Structural Genomics, PSI-2, Protein Structure Initiative, Northeast Structural Genomics Consortium, unknown; HET: PO4; 2.5A {Bacillus subtilis},,,3F3B_A,99.1071,99.3 SIF-Syn: head-to-tail stopper, In same position as Supakev_8, upstream is head-to-tail adaptor, downstream is NKF

CDS 7262 - 7549
  /gene="9"
  /product="gp9"
  /function="hypothetical protein"
  /locus tag="AustinPowers_9"
    /note=Original Glimmer call @bp 7262 has strength 8.47; Genemark calls start at 7262
    /note=SSC: 7262-7549 CP: yes SCS: both ST: SS BLAST-Start: [neck protein [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 8.21621E-58 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.022, -4.551528886052257, no F: hypothetical protein SIF-BLAST: ,,[neck protein [Arthrobacter phage HunterDalle] ],,YP_009602629,98.9474,8.21621E-58 SIF-HHPRED:  SIF-Syn: NKF, In same position as Supakev_8, upstream is head-to-tail stopper, downstream is tail terminator

CDS 7552 - 7983
  /gene="10"
  /product="gp10"
  /function="tail terminator"
  /locus tag="AustinPowers_10"
    /note=Original Glimmer call @bp 7552 has strength 4.76; Genemark calls start at 7552
    /note=SSC: 7552-7983 CP: yes SCS: both ST: SS BLAST-Start:  GAP: 2 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.672, -3.987017166898666, yes F: tail terminator SIF-BLAST:  SIF-HHPRED: TAIL-TO-HEAD JOINING PROTEIN GP17; VIRAL PROTEIN, VIRAL INFECTION, TAILED BACTERIOPHAGE, SIPHOVIRIDAE, SPP1, VIRAL ASSEMBLY, HEAD-TO-TAIL INTERFACE, DNA GATEKEEPER, ALLOSTERIC MECHANISM; 7.2A {BACILLUS PHAGE SPP1},,,5A21_G,89.5105,99.2 SIF-Syn: tail terminator, In same position as Supakev_10, upstream is NKF, downstream is major tail protein

CDS 7980 - 8885
  /gene="11"
  /product="gp11"
  /function="major tail protein"
  /locus tag="AustinPowers_11"
    /note=Original Glimmer call @bp 7980 has strength 16.37; Genemark calls start at 7980
    /note=SSC: 7980-8885 CP: yes SCS: both ST: SS BLAST-Start:  GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.603, -3.368377069717189, yes F: major tail protein SIF-BLAST:  SIF-HHPRED: Major capsid protein; polyvalent staphylococcal bactoriophage, Myoviridae, tail sheath, tail contraction, virus like particle; 3.8A {Staphylococcus phage 812},,,5LII_P,24.2525,94.8 SIF-Syn: major tail protein, in same position as Supakev_11, upstream is tail terminator, downstream is tail assembly chaperone

CDS 8975 - 9382
  /gene="12"
  /product="gp12"
  /function="tail assembly chaperone"
  /locus tag="AustinPowers_12"
    /note=Original Glimmer call @bp 8975 has strength 10.37; Genemark calls start at 8966
    /note=SSC: 8975-9382 CP: yes SCS: both-gl ST: SS BLAST-Start: [tail assembly chaperone [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 1.14406E-90 GAP: 89 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.117, -4.487636275856737, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Arthrobacter phage HunterDalle] ],,YP_009602633,100.0,1.14406E-90 SIF-HHPRED: GP24_25 ; Mycobacteriophage tail assembly protein,,,PF17388.5,68.8889,92.3 SIF-Syn: tail assembly chaperone, in same position as Supakev_12, upstream is major tail protein, downstream is tail assembly chaperone

CDS join(8975..9355,9355..9732)
  /gene="13"
  /product="gp13"
  /function="tail assembly chaperone"
  /locus tag="AustinPowers_13"
    /note=
    /note=SSC: 8975-9732 CP: yes SCS: neither ST: SS BLAST-Start: [tail assembly chaperone [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 0.0 GAP: -408 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.117, -4.487636275856737, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Arthrobacter phage HunterDalle] ],,YP_009602632,100.0,0.0 SIF-HHPRED: DUF5361 ; Family of unknown function (DUF5361),,,PF17318.5,14.6825,96.6 SIF-Syn: tail assembly chaperone, in same position as Supakev_13, upstream is tail assembly chaperone, downstream is tape measure protein.
    /note=this gene was added to properly display gene 13. frame shift present at bp # 9355 and a second gene was erroneously showed to range from 9421 to 9732. this gene was removed due to the frame shift

CDS 9739 - 12297
  /gene="14"
  /product="gp14"
  /function="tape measure protein"
  /locus tag="AustinPowers_14"
    /note=Original Glimmer call @bp 9739 has strength 14.72; Genemark calls start at 9739
    /note=SSC: 9739-12297 CP: yes SCS: both ST: SS BLAST-Start: [tape measure protein [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 0.0 GAP: 5 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.932, -3.5088110057482766, yes F: tape measure protein SIF-BLAST: ,,[tape measure protein [Arthrobacter phage Aledel] ],,AZF98639,100.0,0.0 SIF-HHPRED: Tape Measure Protein, gp57; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_BF,11.5023,99.2 SIF-Syn: tape measure protein, in same position as Supakev_14, upstream is tail assembly chaperone, downstream is minor tail protein

CDS 12294 - 14411
  /gene="15"
  /product="gp15"
  /function="minor tail protein"
  /locus tag="AustinPowers_15"
    /note=Original Glimmer call @bp 12294 has strength 11.0; Genemark calls start at 12294
    /note=SSC: 12294-14411 CP: yes SCS: both ST: SS BLAST-Start:  GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.704, -5.293288977131869, no F: minor tail protein SIF-BLAST:  SIF-HHPRED:  SIF-Syn: minor tail protein, in same position as Supakev_15, upstream is  tape measure protein, downstream is minor tail protein

CDS 14402 - 15559
  /gene="16"
  /product="gp16"
  /function="minor tail protein"
  /locus tag="AustinPowers_16"
    /note=Original Glimmer call @bp 14432 has strength 8.78; Genemark calls start at 14402
    /note=SSC: 14402-15559 CP: yes SCS: both-gm ST: NA BLAST-Start: [minor tail protein [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 0.0 GAP: -10 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.577, -3.5022057636119217, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage HunterDalle] ],,YP_009602636,100.0,0.0 SIF-HHPRED: Sipho_Gp37 ; Siphovirus ReqiPepy6 Gp37-like protein,,,PF14594.9,93.5065,99.7 SIF-Syn: minor tail protein, in same position as Supakev_16, upstream is minor tail protein, downstream is minor tail protein

CDS 15559 - 16584
  /gene="17"
  /product="gp17"
  /function="minor tail protein"
  /locus tag="AustinPowers_17"
    /note=Original Glimmer call @bp 15559 has strength 12.81; Genemark calls start at 15559
    /note=SSC: 15559-16584 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 0.0 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.509, -3.5707972674952195, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage HunterDalle] ],,YP_009602637,100.0,0.0 SIF-HHPRED:  SIF-Syn: minor tail protein, in same position as Supakev_17, upstream is minor tail protein, downstream is minor tail protein

CDS 16584 - 17663
  /gene="18"
  /product="gp18"
  /function="minor tail protein"
  /locus tag="AustinPowers_18"
    /note=Original Glimmer call @bp 16584 has strength 13.45; Genemark calls start at 16584
    /note=SSC: 16584-17663 CP: yes SCS: both ST: SS BLAST-Start:  GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.856, -2.9063687850157054, yes F: minor tail protein SIF-BLAST:  SIF-HHPRED: Receptor Binding Protein; beta sandwich domain, phage receptor binding protein, Lactococcus lactis pellicle cell wall polyphosphosaccharide, VIRAL PROTEIN; 1.75A {Lactococcus phage 1358},,,4L9B_A,47.6323,98.3 SIF-Syn: minor tail protein, in same position as Supakev_18, upstream is minor tail protein, downstream is NKF

CDS 17656 - 18099
  /gene="19"
  /product="gp19"
  /function="hypothetical protein"
  /locus tag="AustinPowers_19"
    /note=Original Glimmer call @bp 17656 has strength 5.58; Genemark calls start at 17656
    /note=SSC: 17656-18099 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 7.33381E-99 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.433, -4.322809268821859, no F: hypothetical protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage HunterDalle] ],,YP_009602639,100.0,7.33381E-99 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_19, upstream is minor tail protein, downstream is NKF
    /note=Featured 4 TMH according to TmHmm

CDS 18099 - 18425
  /gene="20"
  /product="gp20"
  /function="hypothetical protein"
  /locus tag="AustinPowers_20"
    /note=Original Glimmer call @bp 18099 has strength 6.28; Genemark calls start at 18099
    /note=SSC: 18099-18425 CP: yes SCS: both ST: NI BLAST-Start: [hol-like chemotaxis [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 1.75393E-70 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.761, -3.875201829906135, yes F: hypothetical protein SIF-BLAST: ,,[hol-like chemotaxis [Arthrobacter phage HunterDalle] ],,YP_009602640,100.0,1.75393E-70 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_20, upstream is NKF, downstream is endolysin, N-acetylmuramoyl-L-alanine amidase domain

CDS 18422 - 19261
  /gene="21"
  /product="gp21"
  /function="endolysin"
  /locus tag="AustinPowers_21"
    /note=Original Glimmer call @bp 18422 has strength 11.75; Genemark calls start at 18422
    /note=SSC: 18422-19261 CP: yes SCS: both ST: SS BLAST-Start: [amidase [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.738, -3.925516065825927, yes F: endolysin SIF-BLAST: ,,[amidase [Arthrobacter phage HunterDalle] ],,YP_009602641,100.0,0.0 SIF-HHPRED: d.118.1.1 (A:1-157) N-acetylmuramoyl-L-alanine amidase PlyG {Bacillus anthracis [TaxId: 1392]},,,d1yb0a1,44.4444,99.7 SIF-Syn: endolysin, N-acetylmuramoyl-L-alanine amidase domain, in same position as Supakev_21, upstream is NKF, downstream is holin
    /note=N-acetyl-anhydromuramyl-L-alanine amidase
    /note=Evidence includes this domain. Went with overall function.

CDS 19277 - 19780
  /gene="22"
  /product="gp22"
  /function="holin"
  /locus tag="AustinPowers_22"
    /note=Original Glimmer call @bp 19277 has strength 7.47; Genemark calls start at 19277
    /note=SSC: 19277-19780 CP: yes SCS: both ST: SS BLAST-Start: [holin [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 6.55857E-115 GAP: 15 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.19, -5.018480221620088, no F: holin SIF-BLAST: ,,[holin [Arthrobacter phage HunterDalle] ],,YP_009602642,100.0,6.55857E-115 SIF-HHPRED:  SIF-Syn: holin, in same position as Supakev_22, upstream is endolysin with N-acetylmuramoyl-L-alanine amidase domain, downstream is minor tail protein
    /note=Includes transmembrane domains

CDS 19844 - 20983
  /gene="23"
  /product="gp23"
  /function="minor tail protein"
  /locus tag="AustinPowers_23"
    /note=Original Glimmer call @bp 19844 has strength 16.28; Genemark calls start at 19856
    /note=SSC: 19844-20983 CP: yes SCS: both-gl ST: SS BLAST-Start: [tail protein [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 0.0 GAP: 63 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.151, -4.337049294579155, no F: minor tail protein SIF-BLAST: ,,[tail protein [Arthrobacter phage HunterDalle] ],,YP_009602643,100.0,0.0 SIF-HHPRED: CELL WALL SURFACE ANCHOR FAMILY PROTEIN; CELL ADHESION, PFBA, SURFACE ADHESIN, SP1833, MSCRAMM; 2.28A {STREPTOCOCCUS PNEUMONIAE},,,3ZPP_A,39.0501,99.7 SIF-Syn: minor tail protein, in same position as Supakev_23, upstream is holin, downstream is helix-turn-helix DNA binding domain

CDS 21144 - 21452
  /gene="24"
  /product="gp24"
  /function="helix-turn-helix DNA binding domain"
  /locus tag="AustinPowers_24"
    /note=Original Glimmer call @bp 21144 has strength 14.26; Genemark calls start at 21144
    /note=SSC: 21144-21452 CP: yes SCS: both ST: NI BLAST-Start: [HTH DNA binding protein [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 6.20291E-69 GAP: 160 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.952, -2.700465369687878, yes F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[HTH DNA binding protein [Arthrobacter phage HunterDalle] ],,YP_009602644,100.0,6.20291E-69 SIF-HHPRED: a.35.1.2 (L:3-68) P22 C2 repressor, DNA-binding domain {Salmonella bacteriophage P22 [TaxId: 10754]},,,d2r1jl1,65.6863,98.8 SIF-Syn: helix-turn-helix DNA binding domain, in same position as Supakev_24, upstream is minor tail protein, downstream is NKF

CDS 21445 - 21753
  /gene="25"
  /product="gp25"
  /function="hypothetical protein"
  /locus tag="AustinPowers_25"
    /note=Original Glimmer call @bp 21511 has strength 2.08; Genemark calls start at 21445
    /note=SSC: 21445-21753 CP: yes SCS: both-gm ST: NA BLAST-Start:  GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.273, -4.364952153961186, no F: hypothetical protein SIF-BLAST:  SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_25, upstream is helix-turn-helix DNA binding domain, downstream is DNA helicase

CDS 21746 - 23140
  /gene="26"
  /product="gp26"
  /function="DNA helicase"
  /locus tag="AustinPowers_26"
    /note=Genemark calls start at 21746
    /note=SSC: 21746-23140 CP: yes SCS: genemark ST: NI BLAST-Start: [DNA helicase [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 0.0 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.748, -3.058913906516345, no F: DNA helicase SIF-BLAST: ,,[DNA helicase [Arthrobacter phage Aledel] ],,AZF98651,99.7845,0.0 SIF-HHPRED: Chromatin-remodeling ATPase INO80; Chromatin Remodeler, hexasome, DNA BINDING PROTEIN, DNA BINDING PROTEIN-Hydrolase complex; HET: ADP; 3.41A {Saccharomyces cerevisiae S288C},,,8EUF_Q,29.7414,100.0 SIF-Syn: DNA helicase, in same position as Aledel_26, upstream is NKF, downstream is DNA helicase
    /note=No starterator report; HHPred matches chromatin remodeling; ATPase; and DNA binding/repair.
    /note=Removed flanking duplicate based on HHPred hits.

CDS 23296 - 23484
  /gene="27"
  /product="gp27"
  /function="hypothetical protein"
  /locus tag="AustinPowers_27"
    /note=Original Glimmer call @bp 23296 has strength 8.53; Genemark calls start at 23296
    /note=SSC: 23296-23484 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDH58_gp27 [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 9.84831E-37 GAP: 155 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.084, -2.417348306996335, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDH58_gp27 [Arthrobacter phage HunterDalle] ],,YP_009602647,100.0,9.84831E-37 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_27, upstream is DNA helicase, downstream is NKF

CDS 23669 - 24286
  /gene="28"
  /product="gp28"
  /function="hypothetical protein"
  /locus tag="AustinPowers_28"
    /note=Original Glimmer call @bp 23669 has strength 5.39; Genemark calls start at 23669
    /note=SSC: 23669-24286 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDH58_gp29 [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 1.25326E-147 GAP: 184 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.006, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDH58_gp29 [Arthrobacter phage HunterDalle] ],,YP_009602649,100.0,1.25326E-147 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_29, upstream is NKF, downstream is NKF

CDS 24309 - 24800
  /gene="29"
  /product="gp29"
  /function="hypothetical protein"
  /locus tag="AustinPowers_29"
    /note=Original Glimmer call @bp 24369 has strength 6.86; Genemark calls start at 24369
    /note=SSC: 24309-24800 CP: yes SCS: both-cs ST: NI BLAST-Start: [hypothetical protein SEA_ALEDEL_30 [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 4.43292E-115 GAP: 22 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.261, -5.140306621123838, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALEDEL_30 [Arthrobacter phage Aledel] ],,AZF98654,100.0,4.43292E-115 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_30, upstream is NKF, downstream is RuvC-like resolvase

CDS 24781 - 25341
  /gene="30"
  /product="gp30"
  /function="RuvC-like resolvase"
  /locus tag="AustinPowers_30"
    /note=Original Glimmer call @bp 24781 has strength 11.98; Genemark calls start at 24793
    /note=SSC: 24781-25341 CP: yes SCS: both-gl ST: NA BLAST-Start: [hypothetical protein FDH58_gp31 [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 3.0283E-134 GAP: -20 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.509, -3.859592806742189, yes F: RuvC-like resolvase SIF-BLAST: ,,[hypothetical protein FDH58_gp31 [Arthrobacter phage HunterDalle] ],,YP_009602651,100.0,3.0283E-134 SIF-HHPRED: c.55.3.6 (A:) RuvC resolvase {Escherichia coli [TaxId: 562]},,,d1hjra_,81.1828,99.7 SIF-Syn: RuvC-like resolves, in same position as Supakev_31, upstream is NKF, downstream is NKF

CDS 25406 - 25621
  /gene="31"
  /product="gp31"
  /function="hypothetical protein"
  /locus tag="AustinPowers_31"
    /note=Original Glimmer call @bp 25406 has strength 15.84; Genemark calls start at 25406
    /note=SSC: 25406-25621 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDH58_gp32 [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 1.84356E-44 GAP: 64 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.127, -4.4057176022952405, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDH58_gp32 [Arthrobacter phage HunterDalle] ],,YP_009602652,100.0,1.84356E-44 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_32, upstream is RuvC-like resolvase, downstream is NKF

CDS 25728 - 26030
  /gene="32"
  /product="gp32"
  /function="hypothetical protein"
  /locus tag="AustinPowers_32"
    /note=Original Glimmer call @bp 25728 has strength 7.27; Genemark calls start at 25728
    /note=SSC: 25728-26030 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein SEA_ALEDEL_33 [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 2.0262E-64 GAP: 106 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.245, -1.993391246735709, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALEDEL_33 [Arthrobacter phage Aledel] ],,AZF98657,100.0,2.0262E-64 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_33, upstream is NKF, downstream is NKF

CDS 26027 - 26377
  /gene="33"
  /product="gp33"
  /function="hypothetical protein"
  /locus tag="AustinPowers_33"
    /note=Original Glimmer call @bp 26027 has strength 8.16; Genemark calls start at 26027
    /note=SSC: 26027-26377 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein SEA_ALEDEL_34 [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 1.66117E-77 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.989, -2.4787699911121788, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALEDEL_34 [Arthrobacter phage Aledel] ],,AZF98658,100.0,1.66117E-77 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_34, upstream is NKF, downstream is NKF

CDS 26374 - 26619
  /gene="34"
  /product="gp34"
  /function="hypothetical protein"
  /locus tag="AustinPowers_34"
    /note=Original Glimmer call @bp 26374 has strength 8.3; Genemark calls start at 26374
    /note=SSC: 26374-26619 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_SUPAKEV_35 [Arthrobacter phage Supakev]],,NCBI, q1:s1 100.0% 7.24306E-50 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.245, -2.0720764396375664, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_SUPAKEV_35 [Arthrobacter phage Supakev]],,AZS10412,100.0,7.24306E-50 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_35, upstream is NKF, downstream is NKF

CDS 26616 - 27065
  /gene="35"
  /product="gp35"
  /function="hypothetical protein"
  /locus tag="AustinPowers_35"
    /note=Original Glimmer call @bp 26616 has strength 6.95; Genemark calls start at 26616
    /note=SSC: 26616-27065 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein SEA_SUPAKEV_36 [Arthrobacter phage Supakev]],,NCBI, q1:s1 100.0% 3.86876E-99 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.889, -4.897971560974249, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_SUPAKEV_36 [Arthrobacter phage Supakev]],,AZS10413,100.0,3.86876E-99 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_36, upstream is NKF, downstream is NKF

CDS 27065 - 27337
  /gene="36"
  /product="gp36"
  /function="hypothetical protein"
  /locus tag="AustinPowers_36"
    /note=Original Glimmer call @bp 27065 has strength 6.85; Genemark calls start at 27065
    /note=SSC: 27065-27337 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein SEA_ALEDEL_37 [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 1.03782E-56 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.867, -2.740768097004632, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALEDEL_37 [Arthrobacter phage Aledel] ],,AZF98661,98.8889,1.03782E-56 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_37, upstream is NKF, downstream is NKF

CDS 27321 - 27524
  /gene="37"
  /product="gp37"
  /function="hypothetical protein"
  /locus tag="AustinPowers_37"
    /note=Original Glimmer call @bp 27321 has strength 7.34; Genemark calls start at 27321
    /note=SSC: 27321-27524 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ALEDEL_38 [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 2.26513E-38 GAP: -17 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.351, -3.9881460756569185, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALEDEL_38 [Arthrobacter phage Aledel] ],,AZF98662,100.0,2.26513E-38 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_38, upstream is NKF, downstream is Cas4 exonuclease

CDS 27502 - 28449
  /gene="38"
  /product="gp38"
  /function="Cas4 exonuclease"
  /locus tag="AustinPowers_38"
    /note=Original Glimmer call @bp 27502 has strength 13.71; Genemark calls start at 27502
    /note=SSC: 27502-28449 CP: yes SCS: both ST: NA BLAST-Start: [Cas4 family exonuclease [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 0.0 GAP: -23 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.14, -3.461712376159538, yes F: Cas4 exonuclease SIF-BLAST: ,,[Cas4 family exonuclease [Arthrobacter phage Aledel] ],,AZF98663,100.0,0.0 SIF-HHPRED: ATP-dependent DNA helicase (UvrD/REP); DNA, DNA BINDING PROTEIN, DNA BINDING PROTEIN-DNA complex; 3.5A {Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)},,,6PPU_A,92.381,99.7 SIF-Syn: Cas4 exonuclease, in same position as Supakev_39, upstream is NKF, downstream is NKF
Both upstream of recA recombinase
    /note=HHPred includes hit to Msmeg helicase; CDD has 1 domain to nuclease superfamily.  Went with other annotations to Cas4exonuclease.

CDS 28449 - 28724
  /gene="39"
  /product="gp39"
  /function="hypothetical protein"
  /locus tag="AustinPowers_39"
    /note=Original Glimmer call @bp 28449 has strength 6.96; Genemark calls start at 28449
    /note=SSC: 28449-28724 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ALEDEL_40 [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 4.86336E-59 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.878, -4.324286579155733, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALEDEL_40 [Arthrobacter phage Aledel] ],,AZF98664,100.0,4.86336E-59 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_40, upstream is Cas4 exonuclease, downstream is NKF

CDS 28835 - 29878
  /gene="40"
  /product="gp40"
  /function="RecA-like DNA recombinase"
  /locus tag="AustinPowers_40"
    /note=Original Glimmer call @bp 28835 has strength 16.21; Genemark calls start at 28835
    /note=SSC: 28835-29878 CP: yes SCS: both ST: SS BLAST-Start: [RecA-like DNA recombinase [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 0.0 GAP: 110 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.117, -4.408951082954879, no F: RecA-like DNA recombinase SIF-BLAST: ,,[RecA-like DNA recombinase [Arthrobacter phage Aledel] ],,AZF98665,100.0,0.0 SIF-HHPRED: c.37.1.11 (A:1-269) RecA protein, ATPase-domain {Mycobacterium tuberculosis [TaxId: 1773]},,,d1mo6a1,72.6225,99.7 SIF-Syn: RecA-like DNA recombinase, in same position as Supakev_41, upstream is NKF, downstream is NKF

CDS 29937 - 30449
  /gene="41"
  /product="gp41"
  /function="hypothetical protein"
  /locus tag="AustinPowers_41"
    /note=Original Glimmer call @bp 29937 has strength 14.72; Genemark calls start at 29937
    /note=SSC: 29937-30449 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_SUPAKEV_42 [Arthrobacter phage Supakev]],,NCBI, q1:s1 100.0% 4.96644E-121 GAP: 58 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.14, -2.5074698667202133, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_SUPAKEV_42 [Arthrobacter phage Supakev]],,AZS10419,100.0,4.96644E-121 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_42, upstream is RecA-like DNA recombinase, downstream is DNA primase/polymerase/helicase

CDS 30523 - 33156
  /gene="42"
  /product="gp42"
  /function="DNA primase/polymerase/helicase"
  /locus tag="AustinPowers_42"
    /note=Original Glimmer call @bp 30523 has strength 10.67; Genemark calls start at 30985
    /note=SSC: 30523-33156 CP: yes SCS: both-gl ST: SS BLAST-Start: [DNA primase/polymerase/helicase [Arthrobacter phage Supakev] ],,NCBI, q1:s1 100.0% 0.0 GAP: 73 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.366, -3.893834241316366, no F: DNA primase/polymerase/helicase SIF-BLAST: ,,[DNA primase/polymerase/helicase [Arthrobacter phage Supakev] ],,AZS10420,99.3158,0.0 SIF-HHPRED: DNA primase; Helicase, DNA binding, AMPPNP, REPLICATION; HET: ANP; 3.1A {Staphylococcus aureus},,,7OM0_C,38.7685,99.9 SIF-Syn: DNA primase/polymerase/helicase, in same position as Supakev_43, upstream is NKF, downstream is NKF

CDS 33553 - 33786
  /gene="43"
  /product="gp43"
  /function="hypothetical protein"
  /locus tag="AustinPowers_43"
    /note=Original Glimmer call @bp 33553 has strength 7.95; Genemark calls start at 33553
    /note=SSC: 33553-33786 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein SEA_CHRIDISON_43 [Arthrobacter phage Chridison]],,NCBI, q1:s1 100.0% 4.60099E-48 GAP: 396 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.324, -4.811481410788036, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_CHRIDISON_43 [Arthrobacter phage Chridison]],,WAB09096,100.0,4.60099E-48 SIF-HHPRED:  SIF-Syn: NKF, In same position as Supakev_44, upstream is NKF, downstream is DNA polymerase I

CDS 33789 - 35888
  /gene="44"
  /product="gp44"
  /function="DNA polymerase I"
  /locus tag="AustinPowers_44"
    /note=Original Glimmer call @bp 33804 has strength 15.33; Genemark calls start at 33789
    /note=SSC: 33789-35888 CP: yes SCS: both-gm ST: NI BLAST-Start: [DNA polymerase I [Arthrobacter phage Chridison]],,NCBI, q1:s1 100.0% 0.0 GAP: 2 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.489, -4.458092987303931, no F: DNA polymerase I SIF-BLAST: ,,[DNA polymerase I [Arthrobacter phage Chridison]],,WAB09097,100.0,0.0 SIF-HHPRED: Apicoplast DNA polymerase; DNA polymerase, exonulease, apicoplast, Plasmodium falciparum, REPLICATION, TRANSFERASE; HET: PEG, EDO; 2.5A {Plasmodium falciparum (isolate 3D7)},,,7SXQ_A,99.1416,100.0 SIF-Syn: DNA polymerase I, in same position as Supakev_45, upstream is NKF, downstream is adenylosuccinate synthetase PurA-like
    /note=Has the most annotated start.  Went with LO and Genmark

CDS 35955 - 37088
  /gene="45"
  /product="gp45"
  /function="adenylosuccinate synthetase PurA-like"
  /locus tag="AustinPowers_45"
    /note=Original Glimmer call @bp 35922 has strength 9.91; Genemark calls start at 35922
    /note=SSC: 35955-37088 CP: yes SCS: both-cs ST: NI BLAST-Start: [PurA-like adenylosuccinate synthetase [Arthrobacter phage Aledel]],,NCBI, q1:s1 100.0% 0.0 GAP: 66 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.066, -2.66590822473965, yes F: adenylosuccinate synthetase PurA-like SIF-BLAST: ,,[PurA-like adenylosuccinate synthetase [Arthrobacter phage Aledel]],,AZF98670,100.0,0.0 SIF-HHPRED: c.37.1.10 (A:) Adenylosuccinate synthetase, PurA {Escherichia coli [TaxId: 562]},,,d1qf5a_,97.878,100.0 SIF-Syn: adenylosuccinate synthetase PurA-like, in same position as Supakev_46, upstream is DNA Polymerase I, downstream is NKF
    /note=The most called start is 35955; 24 phage had the mcs and called the LO. We went with the better scores. Covers the CP. 1:1 with Aledele

CDS 37226 - 37729
  /gene="46"
  /product="gp46"
  /function="hypothetical protein"
  /locus tag="AustinPowers_46"
    /note=Original Glimmer call @bp 37226 has strength 9.28; Genemark calls start at 37091
    /note=SSC: 37226-37729 CP: yes SCS: both-gl ST: NA BLAST-Start: [hypothetical protein SEA_SUPAKEV_47 [Arthrobacter phage Supakev]],,NCBI, q1:s46 100.0% 2.16549E-108 GAP: 137 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.562, -3.744868061411129, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_SUPAKEV_47 [Arthrobacter phage Supakev]],,AZS10424,76.4151,2.16549E-108 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_47, upstream is adenylosuccinate synthetase PurA-like, downstream is NKF

CDS 37716 - 37928
  /gene="47"
  /product="gp47"
  /function="hypothetical protein"
  /locus tag="AustinPowers_47"
    /note=Original Glimmer call @bp 37716 has strength 8.1; Genemark calls start at 37716
    /note=SSC: 37716-37928 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein FDH58_gp48 [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 7.77057E-43 GAP: -14 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.603, -3.3060637489568596, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDH58_gp48 [Arthrobacter phage HunterDalle] ],,YP_009602668,100.0,7.77057E-43 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_48, upstream is NKF, downstream is deoxyuridine triphosphatase

CDS 37925 - 38842
  /gene="48"
  /product="gp48"
  /function="deoxyuridine triphosphatase"
  /locus tag="AustinPowers_48"
    /note=Original Glimmer call @bp 37925 has strength 12.7; Genemark calls start at 37925
    /note=SSC: 37925-38842 CP: yes SCS: both ST: SS BLAST-Start: [deoxyuridine triphosphatase [Arthrobacter phage Supakev]],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.764, -3.6146798363680817, no F: deoxyuridine triphosphatase SIF-BLAST: ,,[deoxyuridine triphosphatase [Arthrobacter phage Supakev]],,AZS10426,99.6721,0.0 SIF-HHPRED: b.85.4.1 (A:24-159) Deoxyuridine 5`-triphosphate nucleotidohydrolase (dUTPase) {Human (Homo sapiens) [TaxId: 9606]},,,d3ehwa1,46.2295,99.9 SIF-Syn: deoxyuridine triphosphatase, in same position as Supakev_49, upstream is NKF, downstream is NKF

CDS complement (38894 - 39166)
  /gene="49"
  /product="gp49"
  /function="hypothetical protein"
  /locus tag="AustinPowers_49"
    /note=Original Glimmer call @bp 39166 has strength 15.85; Genemark calls start at 39166
    /note=SSC: 39166-38894 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein FDH58_gp50 [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 3.75247E-59 GAP: 119 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.245, -1.9310779259753799, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDH58_gp50 [Arthrobacter phage HunterDalle] ],,YP_009602670,100.0,3.75247E-59 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_50, upstream is deoxyuridine triphosphatase, downstream is NKF

CDS complement (39286 - 39519)
  /gene="50"
  /product="gp50"
  /function="hypothetical protein"
  /locus tag="AustinPowers_50"
    /note=Original Glimmer call @bp 39519 has strength 14.51; Genemark calls start at 39561
    /note=SSC: 39519-39286 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_ALEDEL_51 [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 1.92547E-49 GAP: 125 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.245, -1.9310779259753799, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALEDEL_51 [Arthrobacter phage Aledel] ],,AZF98675,100.0,1.92547E-49 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_51, upstream is NKF, downstream is NKF

CDS complement (39645 - 40223)
  /gene="51"
  /product="gp51"
  /function="hypothetical protein"
  /locus tag="AustinPowers_51"
    /note=Original Glimmer call @bp 40223 has strength 15.97; Genemark calls start at 40223
    /note=SSC: 40223-39645 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ALEDEL_52 [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 4.60569E-132 GAP: 161 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.245, -1.9310779259753799, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALEDEL_52 [Arthrobacter phage Aledel] ],,AZF98676,98.4375,4.60569E-132 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_52, upstream is NKF, downstream is NKF

CDS complement (40385 - 40927)
  /gene="52"
  /product="gp52"
  /function="hypothetical protein"
  /locus tag="AustinPowers_52"
    /note=Original Glimmer call @bp 40927 has strength 14.65; Genemark calls start at 40927
    /note=SSC: 40927-40385 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_SUPAKEV_53 [Arthrobacter phage Supakev]],,NCBI, q1:s1 100.0% 6.22671E-126 GAP: 104 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.006, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_SUPAKEV_53 [Arthrobacter phage Supakev]],,AZS10430,98.3333,6.22671E-126 SIF-HHPRED: Putative transcription protein; helex-turn-helex, transcription factor, DNA binding, TRANSCRIPTION; HET: PO4, GOL, NA, MSE; 2.3A {Pseudomonas aeruginosa PAO1} SCOP: a.4.5.0,,,4GCV_D,66.1111,99.4 SIF-Syn: NKF, in same position as Supakev_53, upstream is NKF, downstream is NKF

CDS complement (41032 - 41250)
  /gene="53"
  /product="gp53"
  /function="hypothetical protein"
  /locus tag="AustinPowers_53"
    /note=Original Glimmer call @bp 41250 has strength 8.29; Genemark calls start at 41460
    /note=SSC: 41250-41032 CP: yes SCS: both-gl ST: NA BLAST-Start: [hypothetical protein SEA_KINGBOB_54 [Arthrobacter phage KingBob]],,NCBI, q1:s1 100.0% 2.36002E-44 GAP: 583 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.16, -2.1123791669543204, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_KINGBOB_54 [Arthrobacter phage KingBob]],,AXH44287,100.0,2.36002E-44 SIF-HHPRED:  SIF-Syn: NKF, in same position as KingBob_44, upstream is NKF, downstream is NKF

CDS 41834 - 42373
  /gene="54"
  /product="gp54"
  /function="hypothetical protein"
  /locus tag="AustinPowers_54"
    /note=Original Glimmer call @bp 41834 has strength 3.88; Genemark calls start at 41744
    /note=SSC: 41834-42373 CP: yes SCS: both-gl ST: NA BLAST-Start: [hypothetical protein SEA_SUPAKEV_55 [Arthrobacter phage Supakev]],,NCBI, q1:s1 100.0% 7.64339E-128 GAP: 583 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.443, -4.5573882134260355, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_SUPAKEV_55 [Arthrobacter phage Supakev]],,AZS10432,100.0,7.64339E-128 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_55, upstream is NKF, downstream is NKF

CDS 42370 - 42696
  /gene="55"
  /product="gp55"
  /function="hypothetical protein"
  /locus tag="AustinPowers_55"
    /note=Original Glimmer call @bp 42370 has strength 5.55; Genemark calls start at 42370
    /note=SSC: 42370-42696 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ALEDEL_56 [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 1.18778E-72 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.234, -2.0162541296952132, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALEDEL_56 [Arthrobacter phage Aledel] ],,AZF98680,100.0,1.18778E-72 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_56, upstream is NKF, downstream is NKF

CDS 42696 - 42842
  /gene="56"
  /product="gp56"
  /function="hypothetical protein"
  /locus tag="AustinPowers_56"
    /note=Genemark calls start at 42696
    /note=SSC: 42696-42842 CP: yes SCS: genemark ST: NA BLAST-Start: [hypothetical protein FDH58_gp57 [Arthrobacter phage HunterDalle] ],,NCBI, q1:s1 100.0% 2.8664E-26 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.748, -3.1358149394540633, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDH58_gp57 [Arthrobacter phage HunterDalle] ],,YP_009602677,100.0,2.8664E-26 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_57, upstream is NKF, downstream is NKF

CDS 42842 - 43006
  /gene="57"
  /product="gp57"
  /function="hypothetical protein"
  /locus tag="AustinPowers_57"
    /note=Original Glimmer call @bp 42842 has strength 6.29; Genemark calls start at 42842
    /note=SSC: 42842-43006 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ALEDEL_58 [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 2.51432E-29 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.989, -2.4787699911121788, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ALEDEL_58 [Arthrobacter phage Aledel] ],,AZF98682,100.0,2.51432E-29 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_58, upstream is NKF, downstream is NKF

CDS 43006 - 43134
  /gene="58"
  /product="gp58"
  /function="hypothetical protein"
  /locus tag="AustinPowers_58"
    /note=Original Glimmer call @bp 43006 has strength 6.25; Genemark calls start at 43006
    /note=SSC: 43006-43134 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein FDH58_gp59 [Arthrobacter phage HunterDalle] ],,NCBI, q1:s2 100.0% 2.41106E-22 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.989, -2.8298788511194775, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein FDH58_gp59 [Arthrobacter phage HunterDalle] ],,YP_009602679,97.6744,2.41106E-22 SIF-HHPRED:  SIF-Syn: NKF, in same position as Supakev_59, upstream is NKF, downstream is HNH endonuclease
    /note=LORF had an incredibly large overlap of 115 bp so it was not selected as the correct start

CDS 43422 - 43682
  /gene="59"
  /product="gp59"
  /function="HNH endonuclease"
  /locus tag="AustinPowers_59"
    /note=Original Glimmer call @bp 43443 has strength 1.56; Genemark calls start at 43422
    /note=SSC: 43422-43682 CP: yes SCS: both-gm ST: NA BLAST-Start: [HNH endonuclease [Arthrobacter phage Aledel] ],,NCBI, q1:s1 100.0% 5.6189E-56 GAP: 287 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.963, -4.73920793635854, yes F: HNH endonuclease SIF-BLAST: ,,[HNH endonuclease [Arthrobacter phage Aledel] ],,AZF98684,100.0,5.6189E-56 SIF-HHPRED:  SIF-Syn: HNH endonuclease, in same position as Supakev_60, upstream is NKF, downstream is NA- last gene
    /note=No starterator; longer reading frames have lower scores.  Small amount of Cp begins at 43400; Went with GeneMark
    /note=HHpred hits to Crispr associated endonucleases.