Mycobacterium phage Gremlin
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| Detailed Information for Phage Gremlin | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Danielle Mila |
| Year Found | 2010 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 42.788058 N, 86.108127 W Map |
| Discovery Notes | Gremlin was obtained from a moist soil sample that was collected at a park. The location that the soil was collected from was approximately two feet away from a stone wall near a construction site and approximately one foot away from a tree in a woodchip filled area that contained decomposing woodchips, leaves, and other organic materials. The depth at which the sample was collected was 10 cm. The sample was collected at 1:33 pm on September 5, 2010 and the temperature was approximately 71 degrees Fahrenheit. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | After being incubated at 37 degrees Celcius for 24 hours, the plaques were clear circles that measured to be between 0.5mm to 1 mm in diameter. The plaques were mostly clear with a slight amount of turbidity at the edges. After 24 hours there was a wider variability in sizes than after 48 hours of incubation. After 48 hours of incubation, the plaques doubled in size and were measured to be between 2 and 2.5 mm in diameter. The plaques were more clear at the 48 hour mark than after 24 hours with the edges of the plaques not displaying any turbidity. There was also a smaller range in plaque sizes and fewer derived as greatly from the mean plaque size. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2010HOPEgremlinDEM |
| Pitt Freezer Box# | 14 |
| Pitt Freezer Box Grid# | 31 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
