Mycobacterium phage KingPhillip3
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| Detailed Information for Phage KingPhillip3 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Robert Sottile |
| Year Found | 2018 |
| Location Found | Las Vegas, NV USA |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 29°C |
| GPS Coordinates | 36.107222 N, 115.143333 W Map |
| Discovery Notes | The soil sample from which this phage was isolated was taken from planter bed 24 at the UNLV recycling and composting center located at the above GPS coordinates taken via iPhone. The local air temperature reported by the NWS located at McCarran International Airport less than one mile from the above location was 85 degrees F. Soil conditions were moist even though local humidity was extremely low due to drip irrigation. The soil was damp but not saturated and clumped together when compressed. The sample was taken between 5 and 10 cm beneath the surface and secured in a plastic bag for transport to the lab. |
| Naming Notes | The initial agar plates created with unenriched lysates from this soil sample produced a few suspicious spots, but when each spot was independently plated, no further phage activity was noted. A name was assigned to each potential plaque, one of these being "Phil." When an enriched sample soil sample was prepared and identically plated, after incubation the entire bacterial population was lysed. Thus, this new phage was named "King Phillip III," for his regal performance eliminating M. Smegmatis. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | King Phillip III creates pin-point plaques when added to naive M. Semgmatis colonies. These tiny plaques tend to cluster and form larger areas of total clearing of approximately 1mm in diameter. The initial plaques have been estimated to be about half that diameter or smaller; the size is beyond the resolution of the measuring device. All plaques are completely clear; there is no evidence of lysogenic activity. At very low concentrations, a halo can be observed around the growing clustered plaques, but this seems to be the result of lower phage volume and not a temperate phage. Plaque morphology has been consistent since initial isolation. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 78 |
| Pitt Freezer Box Grid# | B4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
