Mycobacterium phage MamaRu
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| Detailed Information for Phage MamaRu | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Taylor Vibbert |
| Year Found | 2022 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.990152 N, 86.447268 W Map |
| Discovery Notes | The soil was collected next to my house on College Street, in an area with not much grass other than a few weeds and a gutter. The soil itself was very reddish in color and thin, almost dust like. Some debris. The site experiences a moderate amount of foot traffic and is closer to the backyard parking lot than the road. Purification of the phage occurred through a serial dilution process. It took 5 rounds of purification through serial dilution to see consistent plaque morphology. The titer was low (10^8) in both the 1-plate lysate and 10-plate lysate. |
| Naming Notes | I originally wanted to name this phage RuPaul, who is a drag queen that I admire. That was not available so it is now MamaRu, which is a nickname for RuPaul. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | At first, there was both turbid and clear plaques, averaging at about 1.8 mm. Over time, I started getting more clear plaques that increased in size to about 2-3 mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5 x 10^8 |
| Pitt Freezer Box# | 157 |
| Pitt Freezer Box Grid# | G6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
