CDS 108 - 1124 /gene="1" /product="gp1" /function="endolysin" /locus tag="Janeemi_1" /note=Original Glimmer call @bp 108 has strength 9.47; Genemark calls start at 108 /note=SSC: 108-1124 CP: yes SCS: both ST: SS BLAST-Start: [endolysin [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 0.0 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.245, -2.4406251440021154, yes F: endolysin SIF-BLAST: ,,[endolysin [Arthrobacter phage Phives]],,QOP65129,89.7059,0.0 SIF-HHPRED: ENDOLYSIN; STRUCTURAL PROTEIN, ENDOLYSIN, SECONDARY TRANSLATION PRODUCT, BACTERIOPHAGE; HET: GOL, 1PE; 1.9A {CLOSTRIDIUM PHAGE PHICTP1},,,5A6S_A,60.0592,99.9 SIF-Syn: Same as phives. /note=this is the same as Phives. CDS 1168 - 1620 /gene="2" /product="gp2" /function="terminase, small subunit" /locus tag="Janeemi_2" /note=Original Glimmer call @bp 1168 has strength 13.04; Genemark calls start at 1168 /note=SSC: 1168-1620 CP: yes SCS: both ST: SS BLAST-Start: [terminase small subunit [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 2.85697E-99 GAP: 43 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.154, -2.338663114094478, yes F: terminase, small subunit SIF-BLAST: ,,[terminase small subunit [Arthrobacter phage Phives]],,QOP65130,98.6667,2.85697E-99 SIF-HHPRED: Terminase small subunit; genome packaging, bacteriophage, DNA binding, VIRAL PROTEIN; 1.4A {Enterobacteria phage HK97},,,6Z6E_B,52.0,98.7 SIF-Syn: Same as Phives /note=Same as Phives CDS 1617 - 3323 /gene="3" /product="gp3" /function="terminase, large subunit" /locus tag="Janeemi_3" /note=Original Glimmer call @bp 1617 has strength 13.33; Genemark calls start at 1617 /note=SSC: 1617-3323 CP: yes SCS: both ST: SS BLAST-Start: [terminase large subunit [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 0.0 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.165, -4.394706008439538, no F: terminase, large subunit SIF-BLAST: ,,[terminase large subunit [Arthrobacter phage Phives]],,QOP65131,99.1197,0.0 SIF-HHPRED: Terminase large subunit; genome packaging, bacteriophage, ATPase, nuclease, VIRAL PROTEIN; HET: BR; 2.2A {Enterobacteria phage HK97},,,6Z6D_A,92.9577,100.0 SIF-Syn: Same as Asa16 CDS 3363 - 4736 /gene="4" /product="gp4" /function="portal protein" /locus tag="Janeemi_4" /note=Original Glimmer call @bp 3363 has strength 16.22; Genemark calls start at 3363 /note=SSC: 3363-4736 CP: yes SCS: both ST: SS BLAST-Start: [portal protein [Arthrobacter phage Crewmate]],,NCBI, q1:s1 100.0% 0.0 GAP: 39 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.311, -1.953940808934884, yes F: portal protein SIF-BLAST: ,,[portal protein [Arthrobacter phage Crewmate]],,UIW13256,98.9107,0.0 SIF-HHPRED: PORTAL PROTEIN; BACTERIOPHAGE SPP1, DNA TRANSLOCATION, MOLECULAR MOTOR, VIRAL PORTAL PROTEIN, VIRAL PROTEIN; HET: CA, HG; 3.4A {BACTERIOPHAGE SPP1},,,2JES_Q,91.0285,100.0 SIF-Syn: Same synteny as Crewmate /note=Same as Crewmate CDS 4761 - 6821 /gene="5" /product="gp5" /function="VIP2-like ADP-ribosyltransferase toxin" /locus tag="Janeemi_5" /note=Original Glimmer call @bp 4761 has strength 12.24; Genemark calls start at 4761 /note=SSC: 4761-6821 CP: yes SCS: both ST: SS BLAST-Start: [ADP-ribosyltransferase domain and MuF-like fusion protein [Arthrobacter phage Elezi]],,NCBI, q1:s1 100.0% 0.0 GAP: 24 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.917, -2.9063687850157054, yes F: VIP2-like ADP-ribosyltransferase toxin SIF-BLAST: ,,[ADP-ribosyltransferase domain and MuF-like fusion protein [Arthrobacter phage Elezi]],,QNJ56505,93.9971,0.0 SIF-HHPRED: d.166.1.3 (A:) Eukaryotic mono-ADP-ribosyltransferase ART2.2 {Norway rat (Rattus norvegicus) [TaxId: 10116]},,,d1gxya_,35.4227,99.9 SIF-Syn: Same as Eraser /note=Same as Eraser CDS 6878 - 7237 /gene="6" /product="gp6" /function="hypothetical protein" /locus tag="Janeemi_6" /note=Original Glimmer call @bp 6878 has strength 9.34; Genemark calls start at 6878 /note=SSC: 6878-7237 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_LIZALICA_5 [Arthrobacter phage Lizalica]],,NCBI, q1:s1 98.3193% 5.36471E-67 GAP: 56 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.154, -2.9284886490054283, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_LIZALICA_5 [Arthrobacter phage Lizalica]],,UIW13489,91.5966,5.36471E-67 SIF-HHPRED: SIF-Syn: Same as Lizalica CDS 7290 - 7892 /gene="7" /product="gp7" /function="scaffolding protein" /locus tag="Janeemi_7" /note=Original Glimmer call @bp 7356 has strength 13.56; Genemark calls start at 7356 /note=SSC: 7290-7892 CP: no SCS: both-cs ST: SS BLAST-Start: [scaffolding protein [Arthrobacter phage London] ],,NCBI, q23:s1 89.0% 6.54582E-117 GAP: 52 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.633, -5.411464006628378, no F: scaffolding protein SIF-BLAST: ,,[scaffolding protein [Arthrobacter phage London] ],,QOP64309,98.8764,6.54582E-117 SIF-HHPRED: Scaffold protein; major capsid protein, HK97-like fold, scaffolding protein, procapsid, VIRUS; 3.72A {Staphylococcus phage 80alpha},,,6B0X_f,53.5,98.4 SIF-Syn: this is same as Elezi /note=this is same as Elezi CDS 7920 - 8864 /gene="8" /product="gp8" /function="major capsid protein" /locus tag="Janeemi_8" /note=Original Glimmer call @bp 7920 has strength 15.0; Genemark calls start at 7920 /note=SSC: 7920-8864 CP: no SCS: both ST: NI BLAST-Start: [major capsid protein [Arthrobacter phage Phives]],,NCBI, q1:s1 99.6815% 0.0 GAP: 27 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.212, -2.8084998623841946, yes F: major capsid protein SIF-BLAST: ,,[major capsid protein [Arthrobacter phage Phives]],,QOP65136,95.2532,0.0 SIF-HHPRED: MAJOR CAPSID PROTEIN; VIRUS, MARINE VIRUS, OUTER CAPSID PROTEIN, MATURATION; 4.7A {SYNECHOCOCCUS PHAGE SYN5},,,4BML_C,89.4904,99.9 SIF-Syn: same as phives /note=This is same as Phives CDS 8938 - 9369 /gene="9" /product="gp9" /function="head-to-tail adaptor" /locus tag="Janeemi_9" /note=Original Glimmer call @bp 8938 has strength 8.13; Genemark calls start at 8938 /note=SSC: 8938-9369 CP: yes SCS: both ST: SS BLAST-Start: [head-to-tail adaptor [Arthrobacter phage Elezi] ],,NCBI, q1:s1 93.007% 5.46654E-74 GAP: 73 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.311, -2.0162541296952132, yes F: head-to-tail adaptor SIF-BLAST: ,,[head-to-tail adaptor [Arthrobacter phage Elezi] ],,QNJ56509,98.5185,5.46654E-74 SIF-HHPRED: gp15; Head-Tail Connector Protein gp15 of Bacteriophage SPP1 and similar proteins. The bacteriophage SPP1 gp15 protein is critical in the assembly of the connector, a specialized structure that serves as an interface for head and tail attachment, as well as a point at which DNA exits the head during infection by the bacteriophage.,,,cd08055,72.028,98.4 SIF-Syn: same as Asa16 /note=This is same as Asa16 CDS 9359 - 9490 /gene="10" /product="gp10" /function="hypothetical protein" /locus tag="Janeemi_10" /note=Original Glimmer call @bp 9359 has strength 14.64; Genemark calls start at 9359 /note=SSC: 9359-9490 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_POWERPUFF_9 [Arthrobacter phage Powerpuff] ],,NCBI, q1:s1 83.7209% 2.08837E-13 GAP: -11 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.16, -4.6767843745817785, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_POWERPUFF_9 [Arthrobacter phage Powerpuff] ],,QGZ17307,97.2222,2.08837E-13 SIF-HHPRED: SIF-Syn: Same as Lego CDS 9466 - 9813 /gene="11" /product="gp11" /function="head-to-tail stopper" /locus tag="Janeemi_11" /note=Original Glimmer call @bp 9466 has strength 13.82; Genemark calls start at 9466 /note=SSC: 9466-9813 CP: no SCS: both ST: SS BLAST-Start: [head-to-tail stopper [Arthrobacter phage Lizalica]],,NCBI, q1:s1 100.0% 1.14493E-67 GAP: -25 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.917, -2.8454123590742793, yes F: head-to-tail stopper SIF-BLAST: ,,[head-to-tail stopper [Arthrobacter phage Lizalica]],,UIW13494,93.913,1.14493E-67 SIF-HHPRED: HEAD COMPLETION PROTEIN GP16; VIRAL PROTEIN, VIRAL INFECTION, TAILED BACTERIOPHAGE, SIPHOVIRIDAE, SPP1, VIRAL ASSEMBLY, HEAD-TO-TAIL INTERFACE, DNA GATEKEEPER, ALLOSTERIC MECHANISM; 7.2A {BACILLUS PHAGE SPP1},,,5A21_E,95.6522,99.7 SIF-Syn: Same with Phives /note=Found in many AZ cluster phages CDS 9825 - 10130 /gene="12" /product="gp12" /function="hypothetical protein" /locus tag="Janeemi_12" /note=Original Glimmer call @bp 9825 has strength 10.07; Genemark calls start at 9825 /note=SSC: 9825-10130 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_POWERPUFF_11 [Arthrobacter phage Powerpuff] ],,NCBI, q1:s1 99.0099% 5.76467E-55 GAP: 11 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.311, -2.033982896655645, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_POWERPUFF_11 [Arthrobacter phage Powerpuff] ],,QGZ17309,94.1176,5.76467E-55 SIF-HHPRED: SIF-Syn: Same as Lego /note=Matches to hypothetical protein functions with poor e values CDS 10130 - 10543 /gene="13" /product="gp13" /function="tail terminator" /locus tag="Janeemi_13" /note=Original Glimmer call @bp 10130 has strength 7.61; Genemark calls start at 10130 /note=SSC: 10130-10543 CP: no SCS: both ST: SS BLAST-Start: [tail terminator [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 9.30499E-91 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.75, -3.1085431521568267, yes F: tail terminator SIF-BLAST: ,,[tail terminator [Arthrobacter phage Phives]],,QOP65141,97.8102,9.30499E-91 SIF-HHPRED: TAIL-TO-HEAD JOINING PROTEIN GP17; VIRAL PROTEIN, VIRAL INFECTION, TAILED BACTERIOPHAGE, SIPHOVIRIDAE, SPP1, VIRAL ASSEMBLY, HEAD-TO-TAIL INTERFACE, DNA GATEKEEPER, ALLOSTERIC MECHANISM; 7.2A {BACILLUS PHAGE SPP1},,,5A21_G,95.6204,99.4 SIF-Syn: Same with Phives /note=1 to 1 with Phives. Consistently averaged the function tail terminator . High frequency of function CDS 10558 - 11109 /gene="14" /product="gp14" /function="major tail protein" /locus tag="Janeemi_14" /note=Original Glimmer call @bp 10558 has strength 13.92; Genemark calls start at 10558 /note=SSC: 10558-11109 CP: no SCS: both ST: NI BLAST-Start: [major tail protein [Arthrobacter phage Phives]],,NCBI, q1:s1 99.4536% 1.07672E-121 GAP: 14 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.322, -2.58321678164666, yes F: major tail protein SIF-BLAST: ,,[major tail protein [Arthrobacter phage Phives]],,QOP65142,95.6284,1.07672E-121 SIF-HHPRED: YSD1_22 major tail protein; Bacteriophage tail, helical assembly, VIRAL PROTEIN; 3.5A {Bacteriophage sp.},,,6XGR_M,92.3497,98.6 SIF-Syn: same as phives /note=this is same as phives CDS 11204 - 11476 /gene="15" /product="gp15" /function="tail assembly chaperone" /locus tag="Janeemi_15" /note=Original Glimmer call @bp 11204 has strength 17.14; Genemark calls start at 11204 /note=SSC: 11204-11476 CP: no SCS: both ST: NI BLAST-Start: [tail assembly chaperone [Arthrobacter phage Kaylissa]],,NCBI, q1:s1 100.0% 9.52088E-55 GAP: 94 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.322, -2.58321678164666, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Arthrobacter phage Kaylissa]],,QXO14548,98.8889,9.52088E-55 SIF-HHPRED: SIF-Syn: same as kaylissa /note=this is same as Kaylissa CDS join(11204..11470,11470..11808) /gene="16" /product="gp16" /function="tail assembly chaperone" /locus tag="Janeemi_16" /note= /note=SSC: 11204-11808 CP: yes SCS: neither ST: SS BLAST-Start: [tail assembly chaperone [Arthrobacter phage Kaylissa]],,NCBI, q1:s1 100.0% 7.88866E-136 GAP: -273 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.322, -2.58321678164666, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Arthrobacter phage Kaylissa]],,QXO14549,98.5075,7.88866E-136 SIF-HHPRED: SIF-Syn: Identified by synteny to Phives and other AZ phages. /note=Tail assembly chaperone annotated in PECAAN. CDS 11821 - 14298 /gene="17" /product="gp17" /function="tape measure protein" /locus tag="Janeemi_17" /note=Original Glimmer call @bp 11821 has strength 11.45; Genemark calls start at 11821 /note=SSC: 11821-14298 CP: no SCS: both ST: SS BLAST-Start: [tape measure protein [Arthrobacter phage London] ],,NCBI, q1:s1 99.8788% 0.0 GAP: 11 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.322, -2.0720764396375664, yes F: tape measure protein SIF-BLAST: ,,[tape measure protein [Arthrobacter phage London] ],,QOP64319,85.2713,0.0 SIF-HHPRED: Tape Measure Protein, gp57; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_AF,20.0,100.0 SIF-Syn: Eraser /note=1 to1 with eraser. 100% probability of function of tape measure protein. CDS 14291 - 15166 /gene="18" /product="gp18" /function="minor tail protein" /locus tag="Janeemi_18" /note=Original Glimmer call @bp 14291 has strength 9.5; Genemark calls start at 14291 /note=SSC: 14291-15166 CP: no SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage London] ],,NCBI, q1:s1 100.0% 0.0 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.917, -2.7653702713535186, yes F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage London] ],,QOP64320,94.8454,0.0 SIF-HHPRED: Distal Tail Protein, gp58; phage tail, tail tip, tape measure protein, VIRAL PROTEIN; 3.7A {Staphylococcus virus 80alpha},,,6V8I_BD,97.2509,100.0 SIF-Syn: Eraser /note=1 to1 with Eraser. 100% frequency appearance as minor tail protein for AZ cluster. CDS 15179 - 16174 /gene="19" /product="gp19" /function="minor tail protein" /locus tag="Janeemi_19" /note=Original Glimmer call @bp 15179 has strength 7.61; Genemark calls start at 15179 /note=SSC: 15179-16174 CP: no SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage Elezi] ],,NCBI, q1:s1 99.6979% 0.0 GAP: 12 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.322, -2.0720764396375664, yes F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage Elezi] ],,QNJ56518,95.7576,0.0 SIF-HHPRED: Receptor Binding Protein; beta sandwich domain, phage receptor binding protein, Lactococcus lactis pellicle cell wall polyphosphosaccharide, VIRAL PROTEIN; 1.75A {Lactococcus phage 1358},,,4L9B_A,54.9849,99.4 SIF-Syn: Eraser /note=1 to 1 with eraser. 90% in frequency in function minor tail protein. CDS 16175 - 17617 /gene="20" /product="gp20" /function="minor tail protein" /locus tag="Janeemi_20" /note=Original Glimmer call @bp 16175 has strength 10.14; Genemark calls start at 16175 /note=SSC: 16175-17617 CP: no SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 0.0 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.917, -3.1164791313608173, yes F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage Phives]],,QOP65148,93.3333,0.0 SIF-HHPRED: Protein gp18; NP_465809.1, prophage tail protein gp18, Structural Genomics, Joint Center for Structural Genomics, JCSG, Protein Structure Initiative; HET: MSE, MLY; 1.7A {Listeria monocytogenes EGD-e},,,3GS9_A,98.5417,99.5 SIF-Syn: The genome map corresponds directly with that of Phives, where the minor tail protein gene is in between genes of the same phams in both phage genomes. /note=Because the gene is very closely aligned with that of the minor tail protein in Phives in the same general location, it is assumed that the function called is that of the minor tail protein. The BLAST results also show that most of the genes with the similar sequence in the AZ cluster have this function called as well. HHPred also shows that the overall protein structure resembles that of a minor tail protein CDS 17623 - 20508 /gene="21" /product="gp21" /function="minor tail protein" /locus tag="Janeemi_21" /note=Original Glimmer call @bp 17623 has strength 9.34; Genemark calls start at 17623 /note=SSC: 17623-20508 CP: no SCS: both ST: SS BLAST-Start: [minor tail protein [Arthrobacter phage Phives]],,NCBI, q15:s15 98.5432% 0.0 GAP: 5 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.892, -3.4678233629445905, yes F: minor tail protein SIF-BLAST: ,,[minor tail protein [Arthrobacter phage Phives]],,QOP65149,91.4494,0.0 SIF-HHPRED: SIF-Syn: Same as Eraser /note=Same as Eraser CDS 20520 - 20855 /gene="22" /product="gp22" /function="hypothetical protein" /locus tag="Janeemi_22" /note=Original Glimmer call @bp 20520 has strength 9.27; Genemark calls start at 20520 /note=SSC: 20520-20855 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_22 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 3.19623E-70 GAP: 11 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.322, -1.993391246735709, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_22 [Arthrobacter phage Phives]],,QOP65150,99.0991,3.19623E-70 SIF-HHPRED: SIF-Syn: Matches with Phives /note=Phives matches best CDS 20928 - 21395 /gene="23" /product="gp23" /function="membrane protein" /locus tag="Janeemi_23" /note=Original Glimmer call @bp 20928 has strength 9.3; Genemark calls start at 20928 /note=SSC: 20928-21395 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Arthrobacter phage Elezi] ],,NCBI, q1:s1 100.0% 2.12047E-81 GAP: 72 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.793, -3.6716023243868, yes F: membrane protein SIF-BLAST: ,,[membrane protein [Arthrobacter phage Elezi] ],,QNJ56522,95.4839,2.12047E-81 SIF-HHPRED: SIF-Syn: Different Synteny /note=Same as Elizi CDS 21398 - 21829 /gene="24" /product="gp24" /function="membrane protein" /locus tag="Janeemi_24" /note=Original Glimmer call @bp 21386 has strength 12.81; Genemark calls start at 21398 /note=SSC: 21398-21829 CP: yes SCS: both-gm ST: SS BLAST-Start: [membrane protein [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 1.03477E-94 GAP: 2 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.477, -4.021779273157878, no F: membrane protein SIF-BLAST: ,,[membrane protein [Arthrobacter phage Phives]],,QOP65152,99.3007,1.03477E-94 SIF-HHPRED: SIF-Syn: Same as phives /note=Start site changed to 21398, however the decision is unclear as the Starterator report is inconclusive, and the LORF and Genemark does not support this change. CDS 21813 - 22058 /gene="25" /product="gp25" /function="membrane protein" /locus tag="Janeemi_25" /note=Original Glimmer call @bp 21813 has strength 13.61; Genemark calls start at 21837 /note=SSC: 21813-22058 CP: no SCS: both-gl ST: SS BLAST-Start: [membrane protein [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 8.74623E-39 GAP: -17 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.212, -2.297359520375101, yes F: membrane protein SIF-BLAST: ,,[membrane protein [Arthrobacter phage Phives]],,QOP65153,95.0,8.74623E-39 SIF-HHPRED: SIF-Syn: Same as Phives /note=TMHMM identifies two transmembrane domains. Call it a membrane protein but otherwise function is unknown. CDS 22219 - 22833 /gene="26" /product="gp26" /function="deoxynucleoside monophosphate kinase" /locus tag="Janeemi_26" /note=Original Glimmer call @bp 22219 has strength 14.56; Genemark calls start at 22219 /note=SSC: 22219-22833 CP: yes SCS: both ST: SS BLAST-Start: [deoxynucleoside monophosphate kinase [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 9.26243E-142 GAP: 160 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.16, -4.387988835334809, yes F: deoxynucleoside monophosphate kinase SIF-BLAST: ,,[deoxynucleoside monophosphate kinase [Arthrobacter phage Phives]],,QOP65154,99.5098,9.26243E-142 SIF-HHPRED: c.37.1.1 (A:) Deoxynucleoside monophosphate kinase {Bacteriophage T4 [TaxId: 10665]},,,d1deka_,93.1373,99.9 SIF-Syn: Same as Phives CDS 22935 - 23522 /gene="27" /product="gp27" /function="hypothetical protein" /locus tag="Janeemi_27" /note=Original Glimmer call @bp 22935 has strength 7.22; Genemark calls start at 22935 /note=SSC: 22935-23522 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_27 [Arthrobacter phage Phives]],,NCBI, q1:s1 98.4615% 2.41914E-111 GAP: 101 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 0.917, -7.028301864955391, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_27 [Arthrobacter phage Phives]],,QOP65155,90.1042,2.41914E-111 SIF-HHPRED: SIF-Syn: Matches Phives /note=No AZ phage calls a function CDS 23727 - 24560 /gene="28" /product="gp28" /function="exonuclease" /locus tag="Janeemi_28" /note=Original Glimmer call @bp 23727 has strength 18.83; Genemark calls start at 23727 /note=SSC: 23727-24560 CP: yes SCS: both ST: SS BLAST-Start: [exonuclease [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 0.0 GAP: 204 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.154, -2.356391881054909, yes F: exonuclease SIF-BLAST: ,,[exonuclease [Arthrobacter phage Phives]],,QOP65156,99.278,0.0 SIF-HHPRED: Mitochondrial genome maintenance exonuclease 1; human MGME1, DNA complex, DNA exonuclease, DNA BINDING PROTEIN; 2.702A {Homo sapiens},,,5ZYT_C,83.3935,99.8 SIF-Syn: Same as Phives /note=For the start site, the longest ORF was not used. The shortest gap was also not used. HHPred only shows proteins for homo sapiens. /note= /note=No TmHmm CDS 24560 - 24661 /gene="29" /product="gp29" /function="hypothetical protein" /locus tag="Janeemi_29" /note=Genemark calls start at 24560 /note=SSC: 24560-24661 CP: no SCS: genemark ST: NI BLAST-Start: [hypothetical protein SEA_PHIVES_29 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 3.45735E-12 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.634, -3.4892599424135016, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_29 [Arthrobacter phage Phives]],,QOP65157,100.0,3.45735E-12 SIF-HHPRED: SIF-Syn: same as phives /note=same as phives CDS 24658 - 25026 /gene="30" /product="gp30" /function="nucleoside deoxyribosyltransferase" /locus tag="Janeemi_30" /note=Original Glimmer call @bp 24682 has strength 10.35; Genemark calls start at 24682 /note=SSC: 24658-25026 CP: yes SCS: both-cs ST: SS BLAST-Start: [nucleoside deoxyribosyltransferase [Arthrobacter phage Reedo]],,NCBI, q1:s1 100.0% 1.40083E-70 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.539, -6.654460057720851, no F: nucleoside deoxyribosyltransferase SIF-BLAST: ,,[nucleoside deoxyribosyltransferase [Arthrobacter phage Reedo]],,UJQ86817,93.4426,1.40083E-70 SIF-HHPRED: SIF-Syn: Somewhat similar to Phives /note=HHPred and COG cites hypothetical proteins while NCBI blast claims nucleoside deoxyribosyltransferase as the function (derived from Phives). The start site is in question. CDS 25023 - 25427 /gene="31" /product="gp31" /function="LAGLIDADG endonuclease" /locus tag="Janeemi_31" /note=Original Glimmer call @bp 25023 has strength 12.59; Genemark calls start at 25023 /note=SSC: 25023-25427 CP: yes SCS: both ST: SS BLAST-Start: [LAGLIDADG family homing endonuclease [Arthrobacter sp. EPSL27] ],,NCBI, q1:s1 100.0% 1.18146E-84 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.975, -2.707694805492614, yes F: LAGLIDADG endonuclease SIF-BLAST: ,,[LAGLIDADG family homing endonuclease [Arthrobacter sp. EPSL27] ],,WP_066431302,97.0149,1.18146E-84 SIF-HHPRED: d.95.2.1 (A:) DNA endonuclease I-MsoI {Monomastix sp. cl-1997 [TaxId: 141716]},,,d1m5xa_,75.3731,99.6 SIF-Syn: Matches with Phives. /note=Has the highest identity match with Phives but Phives e value too high. This has a slightly varied motif (LAGLLEGEG), but is similar enough to count as LAGLIDADG endonuclease. CDS 25558 - 26271 /gene="32" /product="gp32" /function="recombination directionality factor" /locus tag="Janeemi_32" /note=Original Glimmer call @bp 25558 has strength 19.47; Genemark calls start at 25558 /note=SSC: 25558-26271 CP: yes SCS: both ST: SS BLAST-Start: [recombination directionality factor [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 6.06347E-171 GAP: 130 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.283, -4.979727136815382, no F: recombination directionality factor SIF-BLAST: ,,[recombination directionality factor [Arthrobacter phage Phives]],,QOP65160,100.0,6.06347E-171 SIF-HHPRED: Gp3-like ; Recombination directionality factor-like,,,PF18897.3,88.1857,100.0 SIF-Syn: Phives /note=1 to 1 phives. High frequency function of recombination directionality factor CDS 26271 - 26420 /gene="33" /product="gp33" /function="hypothetical protein" /locus tag="Janeemi_33" /note=Original Glimmer call @bp 26271 has strength 11.33; Genemark calls start at 26271 /note=SSC: 26271-26420 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_33 [Arthrobacter phage Phives]],,NCBI, q1:s1 97.9592% 2.6793E-23 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.165, -4.455662434380964, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_33 [Arthrobacter phage Phives]],,QOP65161,97.9167,2.6793E-23 SIF-HHPRED: SIF-Syn: Similar to Phives CDS 26490 - 26819 /gene="34" /product="gp34" /function="hypothetical protein" /locus tag="Janeemi_34" /note=Original Glimmer call @bp 26532 has strength 9.8; Genemark calls start at 26532 /note=SSC: 26490-26819 CP: yes SCS: both-cs ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_34 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 1.25185E-70 GAP: 69 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.165, -2.253486910374644, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_34 [Arthrobacter phage Phives]],,QOP65162,98.1651,1.25185E-70 SIF-HHPRED: SIF-Syn: Same as Phives CDS 26823 - 27089 /gene="35" /product="gp35" /function="NrdH-like glutaredoxin" /locus tag="Janeemi_35" /note=Original Glimmer call @bp 26823 has strength 12.65; Genemark calls start at 26823 /note=SSC: 26823-27089 CP: no SCS: both ST: SS BLAST-Start: [NrdH-like glutaredoxin [Arthrobacter phage Adumb2043]],,NCBI, q1:s1 100.0% 1.42546E-51 GAP: 3 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.917, -3.6727816321281046, yes F: NrdH-like glutaredoxin SIF-BLAST: ,,[NrdH-like glutaredoxin [Arthrobacter phage Adumb2043]],,QOP65092,92.0455,1.42546E-51 SIF-HHPRED: c.47.1.1 (A:) Glutaredoxin-like NRDH-redoxin {Escherichia coli [TaxId: 562]},,,d1h75a_,86.3636,99.3 SIF-Syn: same as adumb2043 /note=same as adumb2043 CDS 27086 - 27283 /gene="36" /product="gp36" /function="hypothetical protein" /locus tag="Janeemi_36" /note=Original Glimmer call @bp 27086 has strength 13.81; Genemark calls start at 27086 /note=SSC: 27086-27283 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_36 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 1.29168E-38 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.073, -2.5052746077145835, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_36 [Arthrobacter phage Phives]],,QOP65164,100.0,1.29168E-38 SIF-HHPRED: SIF-Syn: Different Syntheny /note=Same as Phives CDS 27276 - 27488 /gene="37" /product="gp37" /function="hypothetical protein" /locus tag="Janeemi_37" /note=Original Glimmer call @bp 27276 has strength 14.78; Genemark calls start at 27276 /note=SSC: 27276-27488 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_37 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 1.22656E-42 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.684, -3.385117140878946, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_37 [Arthrobacter phage Phives]],,QOP65165,100.0,1.22656E-42 SIF-HHPRED: SIF-Syn: CDS 27485 - 27928 /gene="38" /product="gp38" /function="Holliday junction resolvase" /locus tag="Janeemi_38" /note=Original Glimmer call @bp 27485 has strength 8.95; Genemark calls start at 27485 /note=SSC: 27485-27928 CP: no SCS: both ST: SS BLAST-Start: [holliday junction resolvase [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 4.25683E-94 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.05, -4.692527399714444, yes F: Holliday junction resolvase SIF-BLAST: ,,[holliday junction resolvase [Arthrobacter phage Phives]],,QOP65166,97.2789,4.25683E-94 SIF-HHPRED: HOLLIDAY-JUNCTION RESOLVASE; HYDROLASE, ENZYME, HOMOLOGOUS RECOMBINATION, HOLLIDAY JUNCTION RESOLVING ENZYME, NUCLEASE, ARCHAEA, THERMOPHILE; HET: EDO, SO4; 1.8A {SULFOLOBUS SOLFATARICUS} SCOP: c.52.1.18,,,1OB8_B,72.1088,99.2 SIF-Syn: same as phives /note=same as phives CDS complement (27918 - 28091) /gene="39" /product="gp39" /function="hypothetical protein" /locus tag="Janeemi_39" /note= /note=SSC: 28091-27918 CP: yes SCS: neither ST: NI BLAST-Start: [hypothetical protein SEA_PHIVES_39 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 4.46067E-32 GAP: 179 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.895, -4.871466944371845, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_39 [Arthrobacter phage Phives]],,QOP65167,100.0,4.46067E-32 SIF-HHPRED: SIF-Syn: Single reverse gene found on many other AZ phages in middle of the genome, including Phives. /note=Single reverse gene found on many other AZ phages in middle of the genome. CDS 28271 - 30760 /gene="40" /product="gp40" /function="DNA primase/helicase" /locus tag="Janeemi_40" /note=Original Glimmer call @bp 28271 has strength 12.4; Genemark calls start at 28271 /note=SSC: 28271-30760 CP: yes SCS: both ST: SS BLAST-Start: [DNA primase/helicase [Arthrobacter phage Crewmate]],,NCBI, q1:s1 100.0% 0.0 GAP: 179 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.073, -2.523003374675015, yes F: DNA primase/helicase SIF-BLAST: ,,[DNA primase/helicase [Arthrobacter phage Crewmate]],,UIW13295,95.9036,0.0 SIF-HHPRED: Primase; primase, helicase, ssDNA-binding protein, TRANSFERASE; HET: SO4; 2.406A {Nitratiruptor phage NrS-1},,,6K9C_A,40.2895,100.0 SIF-Syn: same as crewmate /note=same as crewmate CDS 30776 - 30997 /gene="41" /product="gp41" /function="membrane protein" /locus tag="Janeemi_41" /note=Original Glimmer call @bp 30776 has strength 12.2; Genemark calls start at 30776 /note=SSC: 30776-30997 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_41 [Arthrobacter phage Phives]],,NCBI, q1:s1 94.5205% 1.1746E-38 GAP: 15 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.975, -3.473611599459246, yes F: membrane protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_41 [Arthrobacter phage Phives]],,QOP65169,95.8333,1.1746E-38 SIF-HHPRED: SIF-Syn: Same as Phives CDS 30994 - 31110 /gene="42" /product="gp42" /function="hypothetical protein" /locus tag="Janeemi_42" /note=Original Glimmer call @bp 30994 has strength 11.91; Genemark calls start at 30994 /note=SSC: 30994-31110 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_42 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 1.82462E-18 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.477, -3.75071250087134, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_42 [Arthrobacter phage Phives]],,QOP65170,100.0,1.82462E-18 SIF-HHPRED: SIF-Syn: Same as Phives CDS 31325 - 33190 /gene="43" /product="gp43" /function="DNA polymerase I" /locus tag="Janeemi_43" /note=Original Glimmer call @bp 31325 has strength 14.75; Genemark calls start at 31325 /note=SSC: 31325-33190 CP: yes SCS: both ST: SS BLAST-Start: [DNA polymerase I [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 0.0 GAP: 214 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.073, -2.5052746077145835, yes F: DNA polymerase I SIF-BLAST: ,,[DNA polymerase I [Arthrobacter phage Phives]],,QOP65171,99.5169,0.0 SIF-HHPRED: DNA polymerase I; mycobacteria, DNA polymerase, Flap endonuclease, TRANSFERASE; 2.713A {Mycolicibacterium smegmatis},,,6VDE_B,97.1014,100.0 SIF-Syn: Matches Phives /note=Almost all AZ phages call this function. CDS 33187 - 33369 /gene="44" /product="gp44" /function="hypothetical protein" /locus tag="Janeemi_44" /note=Original Glimmer call @bp 33202 has strength 10.98; Genemark calls start at 33202 /note=SSC: 33187-33369 CP: yes SCS: both-cs ST: NI BLAST-Start: [hypothetical protein SEA_ADUMB2043_39 [Arthrobacter phage Adumb2043]],,NCBI, q1:s1 100.0% 2.23436E-23 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.864, -5.075057988041409, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ADUMB2043_39 [Arthrobacter phage Adumb2043]],,QOP65099,80.0,2.23436E-23 SIF-HHPRED: SIF-Syn: Same as Adumb2043 /note=Start site was not strong on Starterator, LORF was not met nor the smallest gap according to Gene Candidates. CDS 33366 - 33668 /gene="45" /product="gp45" /function="DNA ligase" /locus tag="Janeemi_45" /note=Original Glimmer call @bp 33366 has strength 15.63; Genemark calls start at 33366 /note=SSC: 33366-33668 CP: yes SCS: both ST: SS BLAST-Start: [DNA ligase [Arthrobacter phage Phives]],,NCBI, q4:s5 97.0% 4.74383E-60 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.586, -3.7976476850020884, no F: DNA ligase SIF-BLAST: ,,[DNA ligase [Arthrobacter phage Phives]],,QOP65173,94.0594,4.74383E-60 SIF-HHPRED: d.142.2.2 (A:1-314) Adenylation domain of NAD+-dependent DNA ligase {Thermus filiformis [TaxId: 276]},,,d1dgsa3,97.0,99.6 SIF-Syn: Matches Phives /note=Phives had a higher e-value for phages db blast . Query for NCBI blast is not 1 for any. CDS 33665 - 33973 /gene="46" /product="gp46" /function="hypothetical protein" /locus tag="Janeemi_46" /note=Original Glimmer call @bp 33665 has strength 12.71; Genemark calls start at 33665 /note=SSC: 33665-33973 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_46 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 1.31177E-62 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.821, -3.3136498407041004, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_46 [Arthrobacter phage Phives]],,QOP65174,99.0196,1.31177E-62 SIF-HHPRED: SIF-Syn: same as phives /note=This is same as phives CDS 34214 - 35026 /gene="47" /product="gp47" /function="DNA binding protein" /locus tag="Janeemi_47" /note=Original Glimmer call @bp 34214 has strength 19.62; Genemark calls start at 34214 /note=SSC: 34214-35026 CP: yes SCS: both ST: SS BLAST-Start: [DNA binding protein [Arthrobacter phage Crewmate]],,NCBI, q1:s1 99.6296% 1.07525E-135 GAP: 240 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.975, -2.7863799983944713, yes F: DNA binding protein SIF-BLAST: ,,[DNA binding protein [Arthrobacter phage Crewmate]],,UIW13302,90.4943,1.07525E-135 SIF-HHPRED: RNA polymerase sigma factor SigA; DNA-dependent RNA polymerase, nucleotidyl transferase, transcription initiation complex, TRANSCRIPTION; HET: SO4, EDO; 2.76A {Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)},,,5VI8_F,96.2963,100.0 SIF-Syn: same as adumb2043 /note=there is no strong evidence that it is DNA binding protein CDS 35112 - 35516 /gene="48" /product="gp48" /function="helix-turn-helix DNA binding domain" /locus tag="Janeemi_48" /note=Original Glimmer call @bp 35112 has strength 13.32; Genemark calls start at 35112 /note=SSC: 35112-35516 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding domain protein [Arthrobacter phage Phives]],,NCBI, q3:s4 98.5075% 2.81186E-78 GAP: 85 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.793, -5.160958035523474, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding domain protein [Arthrobacter phage Phives]],,QOP65177,89.6296,2.81186E-78 SIF-HHPRED: Putative uncharacterized protein; DNA BINDING PROTEIN; NMR {Hyperthermus butylicus},,,2LVS_A,66.4179,99.0 SIF-Syn: same as phives /note=Same as phives CDS 35565 - 36161 /gene="49" /product="gp49" /function="SprT-like protease" /locus tag="Janeemi_49" /note=Original Glimmer call @bp 35565 has strength 14.8; Genemark calls start at 35565 /note=SSC: 35565-36161 CP: yes SCS: both ST: SS BLAST-Start: [SprT-like protease [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 2.01267E-143 GAP: 48 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.975, -2.707694805492614, yes F: SprT-like protease SIF-BLAST: ,,[SprT-like protease [Arthrobacter phage Phives]],,QOP65178,100.0,2.01267E-143 SIF-HHPRED: SprT-like domain-containing protein Spartan; DPC repair protease, DNA BINDING PROTEIN; HET: ADP, MLZ, FLC; 1.5A {Homo sapiens},,,6MDW_A,49.4949,99.7 SIF-Syn: same as phives /note=same as phives CDS 36281 - 36439 /gene="50" /product="gp50" /function="hypothetical protein" /locus tag="Janeemi_50" /note=Original Glimmer call @bp 36281 has strength 23.21; Genemark calls start at 36281 /note=SSC: 36281-36439 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_POWERPUFF_50 [Arthrobacter phage Powerpuff] ],,NCBI, q1:s1 100.0% 5.92863E-25 GAP: 119 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.245, -2.0895162839948163, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_POWERPUFF_50 [Arthrobacter phage Powerpuff] ],,QGZ17348,100.0,5.92863E-25 SIF-HHPRED: SIF-Syn: same as kaylissa /note=same as kaylissa CDS 36499 - 36801 /gene="51" /product="gp51" /function="hypothetical protein" /locus tag="Janeemi_51" /note=Original Glimmer call @bp 36499 has strength 17.54; Genemark calls start at 36499 /note=SSC: 36499-36801 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_53 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 1.26117E-63 GAP: 59 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.768, -3.0713502170045657, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_53 [Arthrobacter phage Phives]],,QOP65180,99.0,1.26117E-63 SIF-HHPRED: SIF-Syn: same as phives /note=start site changed CDS 36974 - 38386 /gene="52" /product="gp52" /function="serine integrase" /locus tag="Janeemi_52" /note=Original Glimmer call @bp 36962 has strength 16.15; Genemark calls start at 36962 /note=SSC: 36974-38386 CP: yes SCS: both-cs ST: SS BLAST-Start: [serine integrase [Arthrobacter phage YesChef]],,NCBI, q1:s1 100.0% 0.0 GAP: 172 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.453, -3.720984649070371, no F: serine integrase SIF-BLAST: ,,[serine integrase [Arthrobacter phage YesChef]],,QIN94541,94.8827,0.0 SIF-HHPRED: INTEGRASE; HYDROLASE, SERINE RECOMBINASE, UNIDIRECTIONAL, SITE-SPECIFIC RECOMBINATION; 2.15A {STREPTOMYCES PHAGE PHIC31},,,4BQQ_B,64.0426,100.0 SIF-Syn: Same as Elezi /note=Start site changed to 36974. The evidence for this isn`t very strong as there are many strong matches for 36965 too. While the latter has slightly better RBS score, the matches beyond AZ phages suggest the shorter protein. This is a difficult judgement call. CDS 38635 - 38907 /gene="53" /product="gp53" /function="hypothetical protein" /locus tag="Janeemi_53" /note=Original Glimmer call @bp 38635 has strength 11.46; Genemark calls start at 38635 /note=SSC: 38635-38907 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_LEGO_50 [Arthrobacter phage Lego] ],,NCBI, q1:s1 95.5556% 7.25039E-47 GAP: 248 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.073, -2.5052746077145835, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_LEGO_50 [Arthrobacter phage Lego] ],,QIN94450,87.234,7.25039E-47 SIF-HHPRED: SIF-Syn: different than kaylissa /note=same as kaylissa CDS 38904 - 39122 /gene="54" /product="gp54" /function="hypothetical protein" /locus tag="Janeemi_54" /note=Original Glimmer call @bp 38904 has strength 13.0; Genemark calls start at 38904 /note=SSC: 38904-39122 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_56 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 8.40102E-41 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.311, -2.0949393225970705, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_56 [Arthrobacter phage Phives]],,QOP65183,97.2222,8.40102E-41 SIF-HHPRED: SIF-Syn: Same as Phives CDS 39119 - 39409 /gene="55" /product="gp55" /function="hypothetical protein" /locus tag="Janeemi_55" /note=Original Glimmer call @bp 39119 has strength 18.17; Genemark calls start at 39119 /note=SSC: 39119-39409 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_57 [Arthrobacter phage Phives]],,NCBI, q1:s1 91.6667% 9.10413E-42 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.477, -3.8116689268127657, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_57 [Arthrobacter phage Phives]],,QOP65184,86.9565,9.10413E-42 SIF-HHPRED: SIF-Syn: Same as Phives CDS 39425 - 39859 /gene="56" /product="gp56" /function="hypothetical protein" /locus tag="Janeemi_56" /note=Original Glimmer call @bp 39425 has strength 16.88; Genemark calls start at 39425 /note=SSC: 39425-39859 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_58 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 9.10817E-87 GAP: 15 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.975, -2.7863799983944713, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_58 [Arthrobacter phage Phives]],,QOP65185,95.7143,9.10817E-87 SIF-HHPRED: SIF-Syn: Same as phives CDS 39872 - 40054 /gene="57" /product="gp57" /function="hypothetical protein" /locus tag="Janeemi_57" /note=Original Glimmer call @bp 39872 has strength 17.03; Genemark calls start at 39872 /note=SSC: 39872-40054 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_WARDA_56 [Arthrobacter phage Warda]],,NCBI, q1:s1 100.0% 1.37058E-31 GAP: 12 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.322, -2.0720764396375664, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_WARDA_56 [Arthrobacter phage Warda]],,UIW13238,96.6667,1.37058E-31 SIF-HHPRED: SIF-Syn: Same as Phives CDS 40054 - 40293 /gene="58" /product="gp58" /function="hypothetical protein" /locus tag="Janeemi_58" /note=Original Glimmer call @bp 40054 has strength 15.9; Genemark calls start at 40054 /note=SSC: 40054-40293 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_60 [Arthrobacter phage Phives]],,NCBI, q1:s1 97.4684% 1.82058E-32 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.898, -3.90505122954242, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_60 [Arthrobacter phage Phives]],,QOP65187,92.9577,1.82058E-32 SIF-HHPRED: SIF-Syn: Same as Phives CDS 40412 - 40753 /gene="59" /product="gp59" /function="hypothetical protein" /locus tag="Janeemi_59" /note=Original Glimmer call @bp 40412 has strength 14.12; Genemark calls start at 40412 /note=SSC: 40412-40753 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_61 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 1.60834E-74 GAP: 118 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.073, -2.5052746077145835, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_61 [Arthrobacter phage Phives]],,QOP65188,99.115,1.60834E-74 SIF-HHPRED: SIF-Syn: same as phives /note=No TmHmm CDS 40891 - 41220 /gene="60" /product="gp60" /function="hypothetical protein" /locus tag="Janeemi_60" /note=Original Glimmer call @bp 40891 has strength 17.57; Genemark calls start at 40891 /note=SSC: 40891-41220 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_62 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 3.48246E-60 GAP: 137 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.975, -2.996490344739583, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_62 [Arthrobacter phage Phives]],,QOP65189,90.8257,3.48246E-60 SIF-HHPRED: SIF-Syn: same as phives /note=No TmHmm CDS 41291 - 41602 /gene="61" /product="gp61" /function="hypothetical protein" /locus tag="Janeemi_61" /note=Original Glimmer call @bp 41291 has strength 14.53; Genemark calls start at 41291 /note=SSC: 41291-41602 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_63 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 8.29985E-68 GAP: 70 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.073, -2.5052746077145835, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_63 [Arthrobacter phage Phives]],,QOP65190,100.0,8.29985E-68 SIF-HHPRED: SIF-Syn: /note=The start site does not have the longest ORF and shortest gap. CDS 41595 - 41951 /gene="62" /product="gp62" /function="hypothetical protein" /locus tag="Janeemi_62" /note=Original Glimmer call @bp 41595 has strength 16.14; Genemark calls start at 41607 /note=SSC: 41595-41951 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_64 [Arthrobacter phage Phives]],,NCBI, q1:s1 100.0% 2.46032E-69 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.073, -3.095100142625534, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_64 [Arthrobacter phage Phives]],,QOP65191,92.3729,2.46032E-69 SIF-HHPRED: SIF-Syn: Same as Phives /note=No TmHmm CDS 41951 - 42229 /gene="63" /product="gp63" /function="hypothetical protein" /locus tag="Janeemi_63" /note=Original Glimmer call @bp 41951 has strength 9.28; Genemark calls start at 41951 /note=SSC: 41951-42229 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ADOLIN_66 [Arthrobacter phage Adolin]],,NCBI, q1:s1 100.0% 4.58704E-50 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.632, -6.064299277646466, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ADOLIN_66 [Arthrobacter phage Adolin]],,QHB36648,93.4783,4.58704E-50 SIF-HHPRED: SIF-Syn: Same as Adolin CDS 42220 - 42393 /gene="64" /product="gp64" /function="hypothetical protein" /locus tag="Janeemi_64" /note=Original Glimmer call @bp 42220 has strength 13.96; Genemark calls start at 42235 /note=SSC: 42220-42393 CP: yes SCS: both-gl ST: SS BLAST-Start: [membrane protein [Arthrobacter phage Adumb2043]],,NCBI, q1:s1 100.0% 5.70671E-25 GAP: -10 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.322, -2.2821867859826788, yes F: hypothetical protein SIF-BLAST: ,,[membrane protein [Arthrobacter phage Adumb2043]],,QOP65123,91.2281,5.70671E-25 SIF-HHPRED: SIF-Syn: Same as Phives /note=Start site changed CDS 42386 - 42550 /gene="65" /product="gp65" /function="hypothetical protein" /locus tag="Janeemi_65" /note=Original Glimmer call @bp 42386 has strength 8.13; Genemark calls start at 42386 /note=SSC: 42386-42550 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_DRSIERRA_63 [Arthrobacter phage DrSierra]],,NCBI, q1:s1 100.0% 1.27734E-11 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.073, -2.5052746077145835, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_DRSIERRA_63 [Arthrobacter phage DrSierra]],,QIG58541,70.3704,1.27734E-11 SIF-HHPRED: SIF-Syn: Same as Lizalica /note=Start site of 42347 does not match up with gene candidate information. CDS 42543 - 42680 /gene="66" /product="gp66" /function="hypothetical protein" /locus tag="Janeemi_66" /note=Original Glimmer call @bp 42543 has strength 10.32; Genemark calls start at 42543 /note=SSC: 42543-42680 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein SEA_DRSIERRA_64 [Arthrobacter phage DrSierra]],,NCBI, q1:s1 100.0% 5.31998E-14 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.045, -4.6236495748204955, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_DRSIERRA_64 [Arthrobacter phage DrSierra]],,QIG58542,91.1111,5.31998E-14 SIF-HHPRED: SIF-Syn: Same as Asa16 /note=Starterator information does not align with start site. Although, the data from Gene Candidate aligns with the 42543 start site. CDS 42677 - 43015 /gene="67" /product="gp67" /function="HNH endonuclease" /locus tag="Janeemi_67" /note=Original Glimmer call @bp 42677 has strength 4.95; Genemark calls start at 42677 /note=SSC: 42677-43015 CP: yes SCS: both ST: SS BLAST-Start: [HNH endonuclease [Arthrobacter phage Phives]],,NCBI, q1:s1 94.6429% 4.49196E-48 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.715, -3.5327587951385078, yes F: HNH endonuclease SIF-BLAST: ,,[HNH endonuclease [Arthrobacter phage Phives]],,QOP65195,82.7273,4.49196E-48 SIF-HHPRED: SIF-Syn: Matches close with Phives. /note=Matches very closely with Phives CDS 43309 - 43509 /gene="68" /product="gp68" /function="hypothetical protein" /locus tag="Janeemi_68" /note=Original Glimmer call @bp 43309 has strength 4.52; Genemark calls start at 43309 /note=SSC: 43309-43509 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_PHIVES_69 [Arthrobacter phage Phives]],,NCBI, q1:s1 98.4848% 5.93702E-34 GAP: 293 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.154, -2.417348306996335, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_PHIVES_69 [Arthrobacter phage Phives]],,QOP65196,95.4545,5.93702E-34 SIF-HHPRED: SIF-Syn: same as phives /note=No TmHmm CDS 43509 - 43811 /gene="69" /product="gp69" /function="HNH endonuclease" /locus tag="Janeemi_69" /note=Original Glimmer call @bp 43509 has strength 0.23 /note=SSC: 43509-43811 CP: yes SCS: glimmer ST: SS BLAST-Start: [HNH endonuclease [Arthrobacter phage DrSierra]],,NCBI, q1:s1 99.0% 6.44516E-52 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.356, -4.000014702296222, yes F: HNH endonuclease SIF-BLAST: ,,[HNH endonuclease [Arthrobacter phage DrSierra]],,QIG58544,95.0,6.44516E-52 SIF-HHPRED: SIF-Syn: Same as Crewmate /note=Found in multiple AZ phages