LOCUS Kels 14830 bp DNA linear 23-JUN-2022 DEFINITION Arthrobacter phage Kels. ACCESSION VERSION KEYWORDS . SOURCE Arthrobacter phage Kels ORGANISM Arthrobacter phage Kels Viruses; dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae. REFERENCE 1 (bases 1 to 14830) AUTHORS Eivazova,E.R., Pollack,M.J., Galindo,M.E., Matzirakis,A.F., Myers,W.D., Redmon,E.K., Rockenbach,O.L., Sasser,A.F., Williams,A.M., Bolton,C.J., Campbell,M.D., Hollingsworth,B.E., Roberson,O.N., Sullivan,A.D., Staggs,L.G., Taylor,A.R., Ball,S.L., Garlena,R.A., Russell,D.A., Jacobs-Sera,D. and Hatfull,G.F. TITLE Direct Submission JOURNAL Submitted (23-JUN-2022) Center for Life Sciences Education, The Ohio State University, 1735 Neil Ave, Columbus, OH 43210, USA COMMENT Phage Isolation and DNA preparation was performed at University of Pittsburgh, Pittsburgh, PA. Annotation analysis was performed by Columbia State Community College. Sequencing by Illumina to approximately 4703x coverage was performed at Pittsburgh Bacteriophage Institute, Pittsburgh, PA Assembly performed with Newbler and Consed software as of January 2021. Supported by Science Education Alliance, Howard Hughes Medical Institute, Chevy Chase, MD. FEATURES Location/Qualifiers source 1..14830 /organism="Arthrobacter phage Kels" /mol_type="genomic DNA" /isolation_source="soil, Pittsburgh, PA" /lab_host="Arthrobacter globiformis B-2979" /country="USA" /lat_lon="40.445278 N 79.953056 W" /collection_date="01-Sep-2017" /collected_by="Megan Ulbrich" /identified_by="Megan Ulbrich" /note="complete genome" gene 44..352 /gene="1" /locus_tag="SEA_KELS_1" CDS 44..352 /gene="1" /locus_tag="SEA_KELS_1" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MTPETAPMLPGFELPATGMSKLEQRCAQHLDMLQKTGLLQDQDQ VMAQLVMDLAHAVAMSAAAGKAAGTALAVKPLMEALEKLPKPATVDRFQQMMQEAGLA " gene 349..1851 /gene="2" /locus_tag="SEA_KELS_2" CDS 349..1851 /gene="2" /locus_tag="SEA_KELS_2" /codon_start=1 /transl_table=11 /product="terminase" /translation="MTAALADPLAGELPELQAALAGSTAAYATRPTPGAPHELREILG TAKLLGRQLMPWQVQVSRVASEKRVDDPRRYRYPIVVITVPRQAGKTTLMRTVLTQRA LKCRNRRAFYTAQTGKDATARWTDLVKDIENGPLSQFVQKRIAAGSQALTFPTGSTIS PFAPTAKSLHGYTPHDVMLDEVFAHDEAAGNDLMGAIKPAQLTLVDKQLWIVSTAGTA DSVFLKSWVDQGRLATQDSGAGVAYFEWSLADGLDPYDPANWIFHPAVGHTITVEDLA DDATSQTAGEWLRAYMNRWTTTQDACMDLVKWAALAGDPAPVPWGQVSVSYEVAHDRS CSAIYAAWTDPTTGKPAVKLVQQGSGAEWLAPAVAKLYVEARPKVIGADDGGPTKAVT DLLRRLPNARSGSNGVEVTTLTARDFATACVSYMGHVEDGSFIHDGDPGHKAAVEVAA TRPMGDSKVFSRRHSRGPIPELIAAAVALRLHEQAPAAMPAPAIYVRGQA" gene 1848..2048 /gene="3" /locus_tag="SEA_KELS_3" CDS 1848..2048 /gene="3" /locus_tag="SEA_KELS_3" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MIRLDATQFSVVAVCSLCPWRSLAGDRLTGAERGRRHNATDHPN DQQTAAAARQQAYRARKAAGRP" gene 2100..3245 /gene="4" /locus_tag="SEA_KELS_4" CDS 2100..3245 /gene="4" /locus_tag="SEA_KELS_4" /codon_start=1 /transl_table=11 /product="portal protein" /translation="MPLWNNPLAKPAGILARAEAAQQEILAGNIVGWDQGPRDAFETL TLANLFGLDYVNVDRATAMSIGAVAKVRKTACGFIGRMPLVAYRGSTVLARQPKIVTQ PEAGRPRFVTMAWVTEALMFYGRAWFTVQERYKEDGRPAVLRWVPEWSAELDTAGNMV KAFGVAVDKADVIRVDGIDEGLLNYGQDTLREARAVDLAAGRAADNPVPSIDLHQTGG DPLTNDQIDALIDRWATNRRAKNGGVSFTNQSVEAKTMGQPVEQLLIDGRNVAALNIA RATGMPAWVVDASVNGSAITYSNSPSRTRELIDYGLAPYMAAIEARFSMDDILPAGTW CRFDYSDLLRGDFAARMDAYKVAKEAEIYTTEELRAMELGRPLEVAE" gene 3242..4870 /gene="5" /locus_tag="SEA_KELS_5" CDS 3242..4870 /gene="5" /locus_tag="SEA_KELS_5" /codon_start=1 /transl_table=11 /product="major capsid and protease fusion protein" /translation="MTTTTRRPALTLTAGEKRPATLELSAGLLTASASARTISGAIVE YGAVGHTSLGPTIFLPGSISAPSPLSKVKMLVQHDTERSVGFLTELTDDGVKPFASFS VPAGAEGDDALTKAANGTRDSFSVGVHVTEYTFDDEGNLIVHASTLKETSLVTIPAFE NALVHDVAASRKDLTMTEEERRRAALAAAQAPGNPAAQAPAAVEAGAAPAAAAEAAAA GQTIPTRHATAAEAQAAPIRVNSRGMDLAAASQLIVKHLQAGLPAVQLTAALSDVIPG DDAGEGFMRPNFIGELWQAYRDDRPLIDAFGTPGKLTGTKVYGWKWDLANRPKVGRYA GNKTDVPSNPLKTVPAESDAQDFAAGWDVARKYIDLGASDFIESVFRGATADYRLQTE TWFGEQILAEATEVTGVTTVLGALSAFNVQAAGIGARLSTLQFGVDAWEEFINLPESQ VPWWLKQQGSVELDGMKGRAGGVTFAANLGLGAGQILGADSRAATYYEAGSVPIRVTA QDIPRGGVDLGVFGYAGAIVHDPRAIWVSDDGLV" gene 4874..5209 /gene="6" /locus_tag="SEA_KELS_6" CDS 4874..5209 /gene="6" /locus_tag="SEA_KELS_6" /codon_start=1 /transl_table=11 /product="head-to-tail adaptor" /translation="MAITAQAVQEWLGLTVTDEAVAAVVPAVNGFVDGLELPGITTTN ADGTTSTAWSAQTELGAKMLAARLYRRRNSPAGVEAITEAGAAFVARYDSDISRLLQL DGFAGPRIG" gene 5213..5575 /gene="7" /locus_tag="SEA_KELS_7" CDS 5213..5575 /gene="7" /locus_tag="SEA_KELS_7" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MAAANLGDALQDIANMVTAAGVPAAVDPRNLNLPGAWVTPNTVG FDVLDADTAHMRFDVYLVTGDHGAVESLNSLGAMLTKVRKPLQVGDAQPVMVNLPNHG AGALPALLISIDAQITKE" gene 5581..5997 /gene="8" /locus_tag="SEA_KELS_8" CDS 5581..5997 /gene="8" /locus_tag="SEA_KELS_8" /codon_start=1 /transl_table=11 /product="major tail protein" /translation="MATEVITVGPGQFTIGSDLALTSFSGQVTSLRLVPSVDVGDAIY VLDGGEVSGDRSESWSVEGTMLQDFGSTDSKTEWLFENRGQDMPFAYAPNSAKGKQIT GTLTVEAIEIGGDVKTKPTSDFEFKLVGPPAIETLP" gene 6000..6377 /gene="9" /locus_tag="SEA_KELS_9" CDS 6000..6377 /gene="9" /locus_tag="SEA_KELS_9" /codon_start=1 /transl_table=11 /product="minor tail protein" /translation="MAGKLYAVVGGAKLRTTLRKAGADLKQLAAVNREVANIVLPAAK ATAPRRSGKLGATVRAGATQKAAIIRAGGPGVPYAPFVHYWRLDGFTPQPWVSLAAQQ TEPIWLARYHAGIERIIDQVTGA" gene 6382..6732 /gene="10" /locus_tag="SEA_KELS_10" CDS 6382..6732 /gene="10" /locus_tag="SEA_KELS_10" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MARLSAPQVLVLVEQPGSDDLTEYTVQTDNRDAIQWDVSRGRRG WPRFDEAPMLYMTFLAWHAMHRTGATRQSFDEFMAAAVEVKVLDQDGNAVDPADATAD DVLANPTQPAAVTG" gene 6861..8684 /gene="11" /locus_tag="SEA_KELS_11" CDS 6861..8684 /gene="11" /locus_tag="SEA_KELS_11" /codon_start=1 /transl_table=11 /product="tape measure protein" /translation="MSRTAVLAVRIITETKDAGKGFKDTSSKLDKFEAKLNKATVPAA AAGAAVLAFAKKTGDMASIAQQNAGAVDSVFKGNAKQVNAFAKTAADKLGLSASSYQQ MASVIGSQMKNMGVPMDKVAGSTNDLIAKGADLAAMFGGSTADAVDALSSLLRGERDP IERYGVSINDAAIKAQKAKMGLKGLTGEADKNATLTATMALLNKQTADATGQFAREAT SAAGAQEIANAKLQDAGAKMGSVFLPAMAAGAAALSSVATWASENSTVLLILAGVIAG VAGAILLVNGALKAWRAATAAVAAVQVVLNAVMAANPIALVVIAVAALVAGLIWAYQN VGWFKDFVDKAFAAVAAVVGAVAKWFGDAWRNAVAAVTGFLAGVGKFFQAVWSGAVGF VTGVVRGFQNTVAGIFRAVAVVVSVVVGTIRNYFAVGFAAVRAVVAGVVSGVRGTIDG IAGTVSRVVGTVRNGLAGAFQFVAGIGRSAFDGIAGAISNVIGWIQNALSWVRDLAGS ITGAVGKALGLGGATSAAGYLMAPAAAAPAGFAGGATSVFSTPSIVAAPRVRAAAPTI INVTVNGALDPTAVGKQIDGILQQHLRRAGVVSNGESPWSR" gene 8672..10474 /gene="12" /locus_tag="SEA_KELS_12" CDS 8672..10474 /gene="12" /locus_tag="SEA_KELS_12" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MVTITNPGLSLAGGVMHPSKDAAFILPTAFAGLQITYGGDSCSD HPQPGSAAGSVFIPAQHAAFYPRLADPVTLRATINGEDTRIFYGTIDDLIVEDVAGDE LPAIIPNSRHMQSLAGWTITQGTSRAGAVALPADYLVDGARVSATGPQPGGLGSYVYI AAPAAPINTARAYVVTAMAQSPVGYPYLSVEWINAAGAVVQTDELYSYYIPPSLQGDY YALHTATFWPPMDAVKACIVAASELDPTAEPWEQAVGIDGLVLHELPAGVYTLPRYKR PAGRYVKFKASDILATAARLIVGDTPWPQQTIENRTAALNDLVPAGAVSFGFGSADPF ALLGPRDIDKQNMLEIYQRILASAGDLAIAATAPARFIAPASLPRFPMVIADDGTGTA VIITDPLVPELPAGAIEYGPLQTDVTTMSNQIRVEYRQITDTMSQTAEDAAQLYSNDE SVAAFGAMARSVTTDLSVVEGSRAEAKARRLAVAQATPFYRMADNVKLVEAAIPEAAN VARLYSADYGFGQLVRIPDAPSLLGQYHRIKAASITFGKPAAVELDIEPSDYAAPEPV SFGETFGQPFDTLTLSAFAGITLGDLTTTSAQEF" gene 10479..10907 /gene="13" /locus_tag="SEA_KELS_13" CDS 10479..10907 /gene="13" /locus_tag="SEA_KELS_13" /codon_start=1 /transl_table=11 /product="minor tail protein" /translation="MDLNGRFPKLLDGDSVYTVKEYSEKMSDALVAELGSIGEPWQKL TLLNGWVDYVGGGGYKTGLWARKVSDNVQISGMIKNGQTGVALAVLPPELRPPYAAMY PAIAAAASAGVVVNDTGNISYLFGPAAPAYLNMTLFIPLV" gene 10924..11391 /gene="14" /locus_tag="SEA_KELS_14" CDS 10924..11391 /gene="14" /locus_tag="SEA_KELS_14" /codon_start=1 /transl_table=11 /product="endolysin, protease M23 domain" /translation="MEYLHPCPGAELIVPYGARRNLYRKLGQLGHNGDDYEAPEGTPV YATAAGTVLHAGWSHDHPWLTHNAGRAVLVSHTGGDEIAGYAHLSAVAVAEGQPVGAG ELLGYVGHTGTAGSDHLHFERLPAVPDFGNGYAGRIKPPAFTENTDQDKDATS" gene 11388..12140 /gene="15" /locus_tag="SEA_KELS_15" CDS 11388..12140 /gene="15" /locus_tag="SEA_KELS_15" /codon_start=1 /transl_table=11 /product="endolysin, peptidase domain" /translation="MRAVQQQWFDKAIGLAMNPDKAYNLQCVDVVDHYAEAIFPKVSW RESVGGVNGAKDLPNVAPATYWTWTANNPRNPGQLPQPGDVVVWAGTALNPYGHTGVC VRADAAGVQVIQQNYNGLANLSAESRHMPYYGPGTGMISGWLRPRPERMPADPPAPKP ATTPPLKMLTVTAAVAVVRTSPFIRPDNIAQAYPSGIRKGAKIAALGYVAGQDPYPAD KSTDNAWIKTKSGYYIWANAVGNSLAGLKKLA" gene 12154..12366 /gene="16" /locus_tag="SEA_KELS_16" CDS 12154..12366 /gene="16" /locus_tag="SEA_KELS_16" /codon_start=1 /transl_table=11 /product="holin" /translation="MASTNTSPSKLPTAKVTAATAATAAVGLTIALVEWLAGVDVPAA VELPALTLATFAAGYIAPAARRGRHA" gene 12359..12631 /gene="17" /locus_tag="SEA_KELS_17" CDS 12359..12631 /gene="17" /locus_tag="SEA_KELS_17" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MPDIIWTPGETVRAIEAIAGTVGRIEQKLDGKISREDLDRYERA QAHREALQDSAIKDLEGEHSKLMFAALGAGLSGAAGLLIALVSRAG" gene complement(12615..12902) /gene="18" /locus_tag="SEA_KELS_18" CDS complement(12615..12902) /gene="18" /locus_tag="SEA_KELS_18" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain" /translation="MATRVQVRKTSIDAAIGQRVASRLAALGMTQWDLAARLGLTQAT VSRKLHGSRPWFATELVAVAGALGMGVGELFGERPAGAVVVGAGRVASLRG" gene 13001..13219 /gene="19" /locus_tag="SEA_KELS_19" CDS 13001..13219 /gene="19" /locus_tag="SEA_KELS_19" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain" /translation="MPVRYRVAGMTATQTEQPLVTAADAAKQLGVSVRTLDRYQGAGL IQPIRAIHNKGARRQFDANDIARLAGKP" gene 13216..13440 /gene="20" /locus_tag="SEA_KELS_20" CDS 13216..13440 /gene="20" /locus_tag="SEA_KELS_20" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MTTHEDWQAARERRDTAATKYVRLTTDTGRRGDPLPVDHPAALQ ALEQFKAADAYMAAIERELDAALQAKRGRA" gene 13437..14213 /gene="21" /locus_tag="SEA_KELS_21" CDS 13437..14213 /gene="21" /locus_tag="SEA_KELS_21" /codon_start=1 /transl_table=11 /product="helix-turn-helix DNA binding domain" /translation="MSIESMAVVLHHSKAGGTDKLVLLGIANHDGDGGAWPSIATLAR YANVEPRAVKACIKRLVERGEITRERQAGGTRQMPDYSRPNLYHLKVVCPPECDRSAQ HRIDRRHPVLSTTPGVPEGTPGGVSGTYPGGGIQTTPKPLTNQPITTLEKSPVASTSP GGKLPCWNCGENVIDATTRPKSYCTSCSSRGMTSRLIPCTHCGSVRKRAYPGEQEFDC GCTAPAAREQQIAEQWENLTPEQVEQIRNNEADQDKVDAR" gene 14210..14551 /gene="22" /locus_tag="SEA_KELS_22" CDS 14210..14551 /gene="22" /locus_tag="SEA_KELS_22" /codon_start=1 /transl_table=11 /product="Hypothetical Protein" /translation="MITYAEAALNYVEAVAVVKAYEQEVTKARAAWHADEARWRDYQA AMDQWQAAKQREHEARQIIDHLDQAARLARGWEHLRAIPHIELHECPGPLPCLTVDGV LVRANPAHGLR" gene 14592..14804 /gene="23" /locus_tag="SEA_KELS_23" CDS 14592..14804 /gene="23" /locus_tag="SEA_KELS_23" /codon_start=1 /transl_table=11 /product="HNH endonuclease" /translation="MATYGWTCHLCGLPINPGQQSADHLLPRKYGGSNELSNLRPAHR LCNSKRGAKLLTDPRARPRDNTAFFQ" ORIGIN 1 ccgacctttt ttccccgacc caaaacaaaa cgaggcacgg ccaatgaccc ccgaaacagc 61 cccaatgctg cccggcttcg agctgccggc gaccggcatg agcaagctag agcagcgttg 121 tgcgcagcac cttgacatgc tgcagaaaac cggcctgttg caagaccagg atcaagtcat 181 ggcgcagctt gtcatggatc ttgcccacgc cgtcgccatg agcgccgccg ctggcaaggc 241 cgccggcacc gcgctagccg tcaagccgct aatggaggcc cttgaaaagc tgcccaagcc 301 ggcgaccgtt gaccggttcc agcagatgat gcaggaggcc ggtctagcat gacggctgcc 361 cttgccgatc cgttggccgg cgagctgccc gagctgcagg ccgcgcttgc cggcagcacc 421 gcggcctatg cgacccggcc gacgccgggc gcgccgcacg agctacgcga gatccttggc 481 acggccaagc tgcttgggcg ccagctcatg ccgtggcagg tgcaagtgtc ccgcgtggcg 541 tcggagaagc gcgtagacga tccgcgccgg taccgctacc cgattgttgt tatcaccgtg 601 cccaggcagg ccggcaagac gaccctcatg cgcaccgtgc taacgcagcg tgccttgaag 661 tgccgcaacc ggcgcgcgtt ctacacggcg cagacaggca aggacgcgac cgcgcgctgg 721 acggatcttg tcaaggacat tgaaaacggg cccttgtccc agttcgtgca gaagcggatt 781 gccgccggca gccaagcctt gacattcccc accggcagca ccattagccc gttcgcgccc 841 acggctaaga gtctgcacgg ctacacgccg cacgacgtca tgctggacga ggtatttgcc 901 cacgacgagg ccgccggcaa tgaccttatg ggcgcgatca agccggcgca gctcacgctt 961 gtcgacaagc agctttggat tgtttcgacg gccggcacgg ctgacagcgt gttcctaaag 1021 tcatgggtag accagggcag gctagcgacc caggacagcg gcgccggcgt cgcctacttt 1081 gagtggagcc ttgccgacgg ccttgacccg tatgacccgg ctaactggat cttccacccg 1141 gccgtggggc acacgatcac cgttgaggat cttgccgacg acgccacgag ccagaccgcc 1201 ggcgagtggt tgcgcgccta catgaatcgc tggacgacga cgcaagacgc gtgcatggat 1261 cttgtcaagt gggcggcgtt ggccggcgat ccggcgccgg taccgtgggg gcaggtatcc 1321 gtctcctacg aggtagccca tgatcggagc tgcagcgcga tctacgcggc gtggactgac 1381 ccgacaacgg gcaagccggc cgtcaagctc gtgcagcagg gcagcggcgc cgagtggttg 1441 gcgccggccg tcgccaagct ctacgtcgag gcccggccca aggtcattgg cgccgacgac 1501 ggcggcccta ccaaggccgt taccgacctg ttgcgccggc tgcccaacgc gcgcagcggc 1561 agcaacggcg tcgaggtcac gacgctgacc gcgcgcgact tcgcaacggc gtgtgtgtcc 1621 tacatggggc acgtcgagga cggctcgttt atccatgacg gcgacccagg gcataaggcc 1681 gccgtcgagg tagccgcgac ccggcccatg ggcgacagca aggtgtttag ccgccggcac 1741 tcacgcggcc cgatacccga gctaatcgcg gccgccgttg cgttgcggct gcacgagcag 1801 gcaccggccg ccatgccggc gccggcgatc tacgttaggg ggcaggcgtg atccggctgg 1861 acgcgaccca atttagcgtc gtggccgtct gcagcttgtg cccgtggcgc agccttgccg 1921 gcgaccggct gaccggcgcc gagcgcggcc gccggcacaa cgcgaccgac caccccaacg 1981 accagcagac cgccgcagcg gcccggcagc aggcctaccg cgcccgaaaa gcagccggcc 2041 ggccataatg accaccatgt aattttcaaa ccgttacggg ggcatacaag attctttgta 2101 tgcccctctg gaataaccca ctagccaagc cggccggcat ccttgcccga gccgaggccg 2161 cgcagcagga aattcttgcc ggcaacatcg tcggctggga tcaaggcccg cgcgacgcgt 2221 ttgagacgtt gacccttgcc aacctgtttg ggcttgatta cgtcaacgtt gaccgggcga 2281 ccgccatgag cattggcgcc gtcgccaagg tacgcaagac cgcgtgcggc ttcatcggcc 2341 gtatgccgct cgtggcctac cggggcagca ccgtccttgc ccggcagccc aagattgtca 2401 cgcagcccga ggccggccgg cccaggttcg tgactatggc ttgggtaact gaagctctca 2461 tgttctacgg ccgtgcatgg ttcaccgtgc aggagcgtta caaggaggac ggccggccgg 2521 ccgtgcttcg ctgggttccc gagtggagcg ccgagctgga caccgccggc aacatggtca 2581 aggcctttgg cgtcgccgtt gacaaggccg acgttatccg cgtggacggc attgacgagg 2641 gcttgctcaa ttacgggcag gacacgctac gcgaggcgcg cgccgtggat cttgccgccg 2701 gccgcgcggc cgacaacccg gtgcccagca ttgacctgca ccagaccggc ggcgacccgc 2761 tgaccaacga ccagattgac gcgctgattg accgttgggc gactaaccgc cgcgccaaaa 2821 acggcggcgt gagcttcact aaccagagcg tcgaggctaa gaccatgggg cagccggtag 2881 agcagctgct tatcgacggc cgcaacgtcg ccgcgctgaa cattgccagg gcgaccggca 2941 tgccggcttg ggtagttgac gcgtcagtga atggcagcgc gatcacgtac agcaacagcc 3001 catcgaggac gcgggagctg atcgactacg gccttgcccc gtacatggcc gccattgagg 3061 cgcgttttag catggacgac attctgccgg ccggcacatg gtgcaggttc gactattccg 3121 accttttgcg cggcgacttc gcggcccgta tggacgcata caaggtagcc aaggaagccg 3181 agatttacac gaccgaggaa ctacgcgcca tggaacttgg gcgcccgttg gaggtagcag 3241 aatgaccacg accacgcgcc ggccggcgct gaccctgacc gccggcgaga aaaggccggc 3301 gactttggag ctgtccgccg gcctgttgac cgccagcgcg tccgccagga cgattagcgg 3361 cgccattgtc gaatatggcg ccgtggggca caccagcctt gggccgacta ttttcctgcc 3421 cggcagtatc agcgcgccgt cgccgctgtc caaggtaaag atgctcgtgc agcacgacac 3481 ggagcgatcc gttgggttcc tgaccgagct gaccgacgac ggcgtaaagc cgttcgcaag 3541 ttttagcgtg ccggccggcg ccgagggcga cgacgcgcta accaaggccg ccaacggcac 3601 ccgcgacagt ttcagcgtgg gcgtgcacgt tacggaatac acgtttgacg acgagggcaa 3661 cttgattgtt cacgcgtcaa cgctgaaaga aacatcactc gttaccatcc cggcgtttga 3721 aaacgcactt gtgcacgacg tagccgcatc acgaaaggat ctaaccatga ccgaggaaga 3781 acgccgccgc gctgcacttg cagcagccca ggcaccgggc aaccctgcag cgcaggcacc 3841 ggccgccgtc gaggccggcg ctgcgccggc cgctgcagcc gaggccgccg ccgccgggca 3901 gaccatcccg acccggcacg cgaccgccgc cgaggcccag gccgcgccga tccgcgtgaa 3961 cagccggggc atggatcttg ccgccgcgtc gcagctcatt gtcaagcacc tgcaggccgg 4021 tcttccggcc gtgcagctca ccgctgccct gtccgacgtg atcccgggcg acgacgccgg 4081 cgagggcttc atgcgcccga acttcatcgg tgagctgtgg caggcctacc gcgacgaccg 4141 gccgctgatt gacgcgttcg gcaccccggg caagctgacc ggcaccaagg tctatggctg 4201 gaagtgggat cttgccaacc ggcccaaggt gggccgctac gccggcaaca agaccgacgt 4261 gccgtccaac ccgctcaaaa ccgtgccggc cgagtccgac gcgcaggact tcgccgccgg 4321 atgggacgtg gcgcgcaagt acatcgatct tggcgcgtcc gacttcatcg aatcggtatt 4381 ccgtggagcg acggccgact accggctgca gaccgaaacg tggttcggtg agcagattct 4441 tgccgaggca accgaggtga ccggcgtgac gaccgtcctt ggcgcgctgt ccgcgttcaa 4501 cgtgcaggcc gccggcatcg gcgcccggct gtccaccctg cagttcggtg ttgacgcgtg 4561 ggaggaattt atcaacctgc ccgagtccca ggtgccgtgg tggttgaagc agcagggcag 4621 cgtcgagctg gacggcatga aaggccgcgc cggcggcgtg acgttcgccg ccaaccttgg 4681 gcttggcgcc gggcagatcc ttggtgccga cagccgcgcc gcgacctact acgaggccgg 4741 cagcgtgccg atccgtgtaa cggcccagga catcccgcgc ggcggcgttg accttggcgt 4801 gttcggctac gccggcgcca ttgtccacga cccgcgcgcg atctgggtat ccgacgacgg 4861 gcttgtctaa gccatggcga tcacagccca ggccgtgcag gagtggttag gcctgaccgt 4921 caccgacgag gccgtggccg ccgtcgtgcc ggccgtcaac ggcttcgtgg acggcttgga 4981 gctgcccggc atcacaacga ccaacgcgga cggcacgacc agcaccgcgt ggtcggcgca 5041 gaccgagctt ggcgccaaga tgctagctgc ccggctttac cgccggcgca acagcccggc 5101 cggcgtcgag gcgatcaccg aggccggcgc cgcgttcgtg gcccggtacg acagcgacat 5161 tagccggcta ttgcagcttg acggcttcgc cggcccgagg atcggttagg ccatggcagc 5221 agcaaaccta ggcgacgcgc tgcaggacat tgccaacatg gtcacggcgg ccggcgtgcc 5281 ggccgccgtt gacccgcgca acctgaactt gccgggggca tgggtaacgc caaacaccgt 5341 gggctttgac gtgctggacg ccgacacggc ccatatgagg tttgacgttt accttgtcac 5401 cggcgaccac ggcgccgtgg agtcactgaa cagccttggc gccatgctga ccaaggtacg 5461 caagccgctg caggtgggcg acgcgcagcc tgtcatggtc aacctgccca accacggcgc 5521 cggcgcgctg ccggcgctgc ttatcagcat cgacgctcaa ataacaaagg agtagccgtt 5581 atggcaactg aggtaatcac cgtaggccca gggcagttca ccattgggtc tgatcttgcc 5641 ctgaccagct ttagtgggca ggtcaccagt cttaggctcg tgccgtccgt ggacgtggga 5701 gacgccattt atgtacttga cggcggcgag gtatccggcg accgttccga atcgtggagc 5761 gtcgagggca ccatgctgca ggacttcggc agcaccgatt ccaagaccga atggttgttc 5821 gagaaccgtg ggcaggatat gccgttcgcc tacgcgccca acagcgccaa gggcaagcag 5881 atcaccggaa cgctgaccgt cgaggctatc gagattggcg gcgacgtaaa gaccaagccg 5941 acctccgatt tcgagttcaa gctcgttggg ccgccggcga ttgagacgct gccctaacca 6001 tggccggcaa actctacgcc gtggtcggcg gcgccaagct gaggacgacg ctacgcaagg 6061 ccggcgccga tctgaaacag cttgccgccg tcaaccgcga ggtagcgaac atcgttttac 6121 ccgctgccaa ggcgaccgcg cccaggcgca gcggcaagct aggcgcgacc gtccgcgccg 6181 gcgcaaccca aaaggccgcg atcatccgag ccggcggccc cggcgtgccc tacgcgccgt 6241 ttgtccacta ttggcggctt gacggcttca caccgcaacc atgggtcagt ctcgcagcgc 6301 agcagaccga accaatatgg ctagcccggt accacgccgg cattgagcgg atcatcgacc 6361 aagtaacagg agcgtaaaac catggcaaga ctgtcagcac cccaggtact cgtgctagtc 6421 gagcagcccg gcagcgacga tctgaccgag tacaccgtgc agaccgacaa ccgcgacgcg 6481 atccaatggg acgtgtcgcg cggccggcgc ggctggcccc ggtttgacga ggcacccatg 6541 ctctacatga cgttccttgc atggcacgcc atgcaccgca ccggcgcgac ccgccagagc 6601 tttgacgagt tcatggccgc cgccgtcgag gtcaaggtgc ttgaccagga cggcaacgcc 6661 gttgacccgg ccgacgcgac ggccgacgac gtgcttgcaa accctaccca gccggcagcc 6721 gttaccggct aattgtcgag cttgccctag agactggcat agcgccggcc gcgtgggcag 6781 ccgagagtac cgaggtcata gcaacggcgt tgaccatcct tgaagaacga gcagcagcac 6841 aacgaaagta ggccggccca atgtcccgca ccgccgttct agccgtccgc atcatcaccg 6901 agaccaagga cgccggcaag ggcttcaagg acacgtctag caagctggac aagtttgagg 6961 ccaagctgaa caaggcaacc gtgccggctg cagccgccgg cgcggccgtc cttgcgttcg 7021 ccaagaaaac cggcgatatg gcgtctatcg cgcagcagaa cgccggcgcc gttgacagcg 7081 tgttcaaggg caacgccaag caggtcaacg cgtttgccaa gaccgcagcg gacaagctag 7141 gcctgtccgc gtcgtcgtat cagcaaatgg cttccgtgat cggctcacag atgaaaaaca 7201 tgggcgtgcc catggacaag gtagccggat ctaccaacga cctgattgcc aagggcgcgg 7261 atcttgccgc catgtttggc ggcagcacgg ccgacgccgt tgacgcgcta tcgagcctgt 7321 tgcgcggcga gcgtgacccc atcgagcggt acggcgtgag tatcaatgac gccgcgatca 7381 aggcgcaaaa ggccaagatg ggtctaaaag ggctcaccgg cgaggccgac aaaaacgcga 7441 ccctgaccgc gaccatggcc ctgttgaata agcagacagc cgacgctacc gggcagtttg 7501 cacgcgaggc gacgagcgcg gccggcgccc aggagatagc caacgccaag ctgcaggacg 7561 ccggcgccaa aatgggcagc gtattcctgc cggccatggc cgccggcgcc gctgccctgt 7621 cgtcggtagc gacgtgggcg agcgaaaaca gcaccgtgct gcttatcctt gccggcgtca 7681 ttgccggcgt ggccggcgcc attctgcttg tcaacggcgc gctgaaagca tggcgcgcgg 7741 caacggccgc cgtcgcggcc gtgcaggtgg tactcaatgc cgtcatggcc gccaacccca 7801 ttgcccttgt tgtcattgcc gtcgccgcgc tcgtggccgg cctgatttgg gcttaccaaa 7861 atgtcgggtg gttcaaggac tttgttgaca aggcctttgc cgccgtggcc gccgtcgtgg 7921 gcgccgtcgc caagtggttt ggcgacgcgt ggcgcaacgc cgttgcggcc gtcaccgggt 7981 tcctagccgg cgtcggcaaa ttcttccagg ccgtgtggag cggcgccgtg ggcttcgtca 8041 ccggcgtcgt gcgaggcttc caaaacaccg tggccggcat cttccgcgca gtggccgtcg 8101 tcgtgtccgt cgtcgtcggc acgatccgaa actacttcgc cgttggcttc gccgccgtcc 8161 gcgccgtagt cgccggcgtc gtctccggcg tccgtggcac catcgacggc atagcgggca 8221 ccgtgtcccg cgtcgtcggc accgtccgca acggcctagc cggcgcgttt cagttcgtcg 8281 ccggcatcgg ccgcagcgcg tttgacggca tagccggcgc catttccaac gtcattggct 8341 ggatccaaaa cgcgctgtct tgggtgcggg atcttgccgg cagcatcacc ggcgccgtgg 8401 gcaaggccct aggccttggc ggggcaacga gcgccgccgg ctacctcatg gcgccggccg 8461 ctgcagcgcc ggccggcttc gccggcggcg caacgagcgt atttagcacg ccaagcattg 8521 tggccgcgcc cagggtacgg gccgccgcgc ccacgattat caacgtcacc gtcaacggcg 8581 cacttgaccc taccgccgtg ggcaagcaga tcgacggcat cctgcagcag cacctacgcc 8641 gcgccggcgt cgtttccaac ggagaatcac catggtcacg ataaccaacc ccggcctgtc 8701 gttagccggc ggtgtcatgc acccgagcaa ggacgccgcg ttcatcctgc ccacggcgtt 8761 tgccggcctg cagatcacct acggcggcga ctcgtgcagc gaccacccac agccaggcag 8821 cgcggccggc agcgtcttta tcccggcgca gcacgccgcg ttctatccgc gcctagccga 8881 tcctgtcacc ttgcgtgcca cgataaacgg cgaggataca cggatctttt acggcaccat 8941 tgacgacctg attgtggagg acgtggccgg cgacgagctg ccggcgatca tcccgaacag 9001 ccggcacatg cagagccttg ccggctggac gatcacccag ggcaccagcc gcgccggcgc 9061 cgtggcgctg ccggcagact accttgtgga cggtgccagg gtatcggcga ccgggccgca 9121 gccgggcggc cttggcagct acgtttacat tgccgcgccg gccgcgccga tcaacacagc 9181 gagggcatac gtcgttacgg ccatggcaca gtcgcccgtt ggctaccctt acctgtccgt 9241 tgagtggatc aacgcggccg gcgccgtcgt gcagaccgac gagctttaca gctactacat 9301 tccgccgtcg ctgcagggcg attactacgc gctgcacacc gcgacctttt ggccgccaat 9361 ggacgccgtc aaggcctgta tcgtggcagc gtccgagctt gacccgacag ccgagccatg 9421 ggagcaggcc gttggcatcg acgggcttgt gctgcacgag ctgccggccg gcgtctacac 9481 tcttcctcgg tacaagcggc cggccggccg ttacgtcaag ttcaaggcat ccgacattct 9541 tgccacggcg gcccggctta tcgtgggaga tacgccatgg ccgcagcaga ccatcgagaa 9601 caggacagcc gcgctcaatg accttgtgcc ggccggcgcc gtgagcttcg gcttcggcag 9661 cgccgacccg tttgccctgc ttgggccgcg cgacattgac aagcagaaca tgctggaaat 9721 ttatcagcgg atcttggcaa gcgccggcga ccttgccatt gccgcgacgg cgccggcccg 9781 gtttatcgct ccggcatcgc tgcccaggtt cccaatggtc atagccgacg acggcaccgg 9841 cacggccgtt attatcaccg atccgctcgt gcccgagctg ccggccggcg cgatagaata 9901 cgggccgctg cagaccgacg taaccacaat gtcaaaccaa atccgcgtgg aataccggca 9961 gattaccgac accatgagcc aaacggccga ggacgccgcg cagctctaca gcaacgacga 10021 atcggtggca gcgttcggcg ccatggcgcg cagcgtcacc accgacctgt cagtggttga 10081 gggcagccgc gccgaggcaa aagcgcgccg gcttgccgtg gcccaagcga cgccgtttta 10141 ccgtatggcc gataacgtca agctcgttga ggcagccata cccgaggccg ccaacgtcgc 10201 acgactttac agcgccgact acgggttcgg gcagctcgtg cggatacccg acgcgccgtc 10261 gctgcttggg cagtaccacc gaatcaaggc cgcatcgatc acctttggca agccggcagc 10321 cgtcgagctg gacattgaac cgtccgacta cgccgcgccc gagcctgtca gcttcgggga 10381 gacgttcggc cagccgtttg acaccttgac cctttcagcc tttgccggca tcacgctagg 10441 cgatctgacc accacgagcg cgcaggagtt ctaacaccat ggatctaaac ggacggtttc 10501 cgaaactgct tgacggcgac agcgtttaca ccgtcaagga atacagcgag aaaatgagcg 10561 acgcgctcgt ggccgagctg ggcagcatcg gtgagccatg gcaaaagctg accctgctaa 10621 acggctgggt tgactacgtc ggcggcggcg gctacaagac cggcctgtgg gcgcgcaagg 10681 tatccgacaa cgtgcaaatc agcggcatga taaaaaacgg gcagaccggc gtagcacttg 10741 cggtgctgcc accagagctt aggccgccat acgcggcaat gtatccggcc atagccgccg 10801 ccgcgtctgc cggcgtcgtc gtgaacgaca ccggcaatat ctcatatttg tttggcccgg 10861 ccgcgccggc ctatctgaac atgacactgt ttattccgct cgtttagtaa ggattatgcc 10921 cctatggaat atctgcaccc ttgccccggc gccgagctga ttgtgcccta cggcgcccgg 10981 cgcaaccttt accgcaagct tgggcagctt gggcacaacg gcgacgacta cgaggcgccc 11041 gagggcacgc cggtctacgc cacggccgcc ggcaccgtgc tgcacgccgg ttggtcacac 11101 gaccaccctt ggctgaccca caacgccggc cgcgccgtgc ttgtctccca taccggcggc 11161 gacgagatag ccggctatgc ccacctgtcg gccgtggccg tcgccgaggg gcagccggta 11221 ggcgccggcg agctgcttgg ctacgtcggg cataccggca ccgccggcag cgaccacctg 11281 cactttgagc ggctgccggc cgtgcccgac ttcggcaacg gctacgccgg ccggatcaag 11341 ccgccggcct ttaccgaaaa caccgaccag gacaaggacg cgacatcatg agagcagtgc 11401 aacagcagtg gttcgacaag gccattggcc ttgccatgaa tccagacaag gcctacaacc 11461 tgcagtgtgt tgacgtagtt gaccactacg ccgaggccat atttcccaaa gtctcgtggc 11521 gcgagtccgt gggcggcgtc aacggcgcca aggatctgcc caacgtcgcc ccggcgacct 11581 actggacgtg gacggcgaac aacccgcgca acccagggca gctaccgcag cccggcgacg 11641 tggttgtgtg ggcaggcacg gcgctaaacc cttacggcca caccggcgtg tgcgtccgcg 11701 ccgacgccgc cggcgtgcag gtgatccagc agaattacaa cggccttgcc aacctgtccg 11761 ccgagtcccg ccacatgccc tactacggcc ccggcaccgg catgattagc gggtggttgc 11821 ggcccaggcc cgagcgtatg ccggccgatc cgccggcgcc caagccggcg accacgccgc 11881 cgttgaaaat gctcaccgtc accgctgccg tggccgtcgt tcgaacgtcg ccgttcatcc 11941 ggccggacaa cattgcccaa gcctatccgt ccggcatccg caagggcgcc aagattgccg 12001 cgctaggcta cgtcgccggc caagacccat acccggccga caagtccacc gataacgcgt 12061 ggatcaagac caagagcggc tactacatat gggcaaacgc cgtgggcaac agccttgccg 12121 gcctgaaaaa actagcgtag ggagtaccct gttatggctt cgaccaacac cagcccgagc 12181 aagctgccca cggccaaggt cacggcggcg accgccgcga cggccgctgt tggcctgacc 12241 attgcccttg tcgagtggtt ggccggcgtt gacgtgccgg ccgccgtcga gctgccggca 12301 ctgacccttg cgacgttcgc cgccggctac attgcccctg cagcacgccg aggacgccat 12361 gcctgacatt atttggacac cgggcgagac agtccgcgct attgaagcca tagccggcac 12421 cgtaggccgg atcgagcaaa agctagacgg caaaataagc cgcgaggatc tagaccgcta 12481 cgagcgcgcc caggcacacc gcgaggcgct gcaggacagc gcaataaagg atctggaggg 12541 cgaacacagc aagctcatgt ttgcggccct tggcgccggc ctgtcgggcg ctgcaggtct 12601 gcttatcgcg ctcgttagcc gcgcaggcta gcgacccggc cggcgccgac gaccacggcg 12661 ccggccggcc gttcgccaaa cagctcaccg acccccatgc ccagggcgcc ggcgaccgcg 12721 acaagttccg tggcgaacca tggccggctg ccgtgcagct tccggctgac cgtggcttgt 12781 gtcagcccca gccgggccgc gagatcccat tgcgtcatgc ccagtgccgc cagcctgcta 12841 gcgacgcgct gcccaatggc cgcgtctatc gaggtcttgc gtacctgcac cctagttgcc 12901 atggcgacac gttacgccat gaaacgacac tctagcgact gtacttagta cctgcttttg 12961 cgcggcgact acttgccggc gtggcgctag ttgtcgtttc atgcctgtcc ggtatagagt 13021 cgccggcatg acagcaaccc aaacagagca gcccctagtc accgctgccg acgccgccaa 13081 gcagcttggc gtgtccgtcc gcacccttga ccgataccag ggcgccggcc ttatccagcc 13141 aatccgagcc attcacaaca agggcgcgcg ccggcagttc gacgccaacg acattgcccg 13201 gttggcaggc aagccatgac aacccatgag gactggcaag cagctcgtga gcgccgcgac 13261 acggccgcaa ccaagtatgt tcggctgacc accgacaccg gccggcgtgg cgacccgctg 13321 ccggtagatc acccggccgc gctgcaggcc ctagagcagt tcaaggctgc cgacgcctat 13381 atggccgcca ttgagcgcga gcttgacgcc gcgctgcagg ccaaacgggg gcgggcatga 13441 gtatcgaatc catggccgtt gtgctgcacc actccaaggc cggcggcact gacaagctcg 13501 tgctgcttgg cattgccaac cacgacggcg acggcggggc atggccgtcc attgcgaccc 13561 ttgcccgata tgccaacgtc gagccgcgcg ccgtcaaggc ctgtatcaag cggcttgtcg 13621 agcgcggcga gattacccga gagcgccaag caggcggcac gcggcaaatg cccgactaca 13681 gccggcccaa cctgtaccac ctgaaagtcg tgtgccctcc cgagtgcgac cgatccgcgc 13741 agcaccgcat tgaccgccgg cacccggtgt tgtcaacgac acccggtgtt cccgagggta 13801 ccccgggggg ggtatctgga acgtaccccg gggggggtat ccagacgaca cccaaaccat 13861 taactaacca accaataacc acccttgaaa aatcacctgt tgctagtacc tcaccagggg 13921 gcaagctgcc gtgctggaat tgcggcgaga acgtcattga cgcgaccacc cggcccaaaa 13981 gctactgcac gagctgcagc agccggggca tgaccagccg acttatccca tgcacccatt 14041 gcggatctgt ccgcaagcgc gcctaccccg gcgagcagga gtttgattgt ggctgcacgg 14101 cgccggcagc tcgtgagcag cagatagccg agcagtggga gaacctgacc cccgagcagg 14161 tagagcagat ccgcaacaac gaagcagacc aagacaaggt ggacgcaaga tgattaccta 14221 cgcagaagca gcattgaatt acgttgaggc cgtggccgtg gtcaaggcct acgagcagga 14281 agtgaccaag gcccgagcag cgtggcacgc cgacgaggcg cgttggcgcg actatcaagc 14341 agccatggat caatggcagg cagccaagca gcgtgagcac gaggcgcgcc agatcattga 14401 ccaccttgac caggcagcca ggctagcccg agggtgggag catctacgcg cgatacctca 14461 cattgaactg cacgagtgcc ccggcccatt gccgtgcctg accgtggacg gcgttctagt 14521 ccgtgccaac cctgcccatg gcctacggtg attggggagg acgtgcagcg cagcggctcg 14581 ttgcgttgtg catggcgacg tatggctgga cgtgtcacct atgcggcttg ccgatcaacc 14641 cggggcaaca gtcagccgac cacctgctac cgcgcaagta tggcggcagc aacgagctgt 14701 ccaacctacg gccggcgcat cgtctgtgca acagcaagcg cggcgccaag ttgctcaccg 14761 acccgcgtgc cagaccgcga gataacaccg cgttttttca gtgacggcgc ccaggacatc 14821 ccgcgcccac //