Mycobacterium phage Alexander
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| Detailed Information for Phage Alexander | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nikita Vispute |
| Year Found | 2013 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.440556 N, 79.996111 W Map |
| Discovery Notes | The soil sample was collected from the backyard of my aunt's house in Pittsburgh on 09.01.2013. The soil sample was damp as the plants in the backyard had just been watered. Soil sample was dark brown in colour. |
| Naming Notes | Alexander The Great was the Macedonian ruler who conquered most of the known world during his time and formed a whole new empire by merging the pre-existing ones. Phage Alexander was named so because phages destroy their host bacteria or sometimes co-exist with the (lysogen) wherein they have new DNA through recombination. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Mycobacteriophage Alexander did not form lysogens. An isolated colony from a streaked M. smeg. plate was mixed with media and plated along with spots of the dilutions of the lysate obtained from the enriched sample of Phage Alexander. Plaques were seen on observation after 2 days. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | About 25-30 plaques on the entire plate plated with Mycobacterium smegmatis host cells. The plaques are large in size but some small plaques also seen. The plaques formed are from one phage only. Average plaque size is 2mm in diameter. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 1 |
| Pitt Freezer Box Grid# | A1 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |