Streptomyces phage Amethyst
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Detailed Information for Phage Amethyst
Discovery Information
Isolation HostStreptomyces xanthochromogenes NRRL B-5410
Found ByElizabeth Spangler
Year Found2016
Location FoundDenton, TX United States
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.2125 N, 97.14722 W Map
Discovery NotesSoil found in Denton, TX; Temperature was 85° F at soil sample collection. The date and time of soil sample collection was 9/9/16 at 11:08am; the soil collected was dry, loose soil.

The MTL of this phage is 1.2x10^8 pfu/mL; the average diameter of plaques is 2mm; the DNA concentration is 131.8 and the DNA/RNA ratio is 1.93.

In a host range test with the bacteria S. venezuelae, S. albus, S. virginiae, S. griseus, and S. azureus, no plaques were formed using Amethyst.
Naming NotesThe name for this phage came from the February birthstone, Amethyst.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 30, 2017
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage312
Genome length (bp)49372
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCGCCACGTCTT
GC Content67.2%
Sequencing Notesat position 19553 approximately 33% of reads contain a G instead of consensus A
Fasta file available?Yes: Download fasta file
Characterization
ClusterBD
SubclusterBD2
Other Cluster Members
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes79
Has been Phamerated?Yes
Gene List

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Amethyst_1

Amethyst_2
(HNH endonuclease)

Amethyst_3

Amethyst_4
(thioredoxin)

Amethyst_5

Amethyst_6
(terminase large subunit)

Amethyst_7
(portal protein)

Amethyst_8

Amethyst_9
(scaffolding protein)

Amethyst_10
(major capsid protein)

Amethyst_11

Amethyst_12
(head-to-tail connector protein)

Amethyst_13

Amethyst_14

Amethyst_15
(major tail protein)

Amethyst_16
(tail assembly chaperone)

Amethyst_17
(tail assembly chaperone)

Amethyst_18
(tape measure protein)

Amethyst_19
(minor tail protein)

Amethyst_20
(minor tail protein)

Amethyst_21

Amethyst_22

Amethyst_23

Amethyst_24
(minor tail protein)

Amethyst_25

Amethyst_26
(lysin)

Amethyst_27

Amethyst_28

Amethyst_29
(immunity repressor)

Amethyst_30

Amethyst_31
(exonuclease)

Amethyst_32

Amethyst_33

Amethyst_34
(deoxycytidylate deaminase)

Amethyst_35
(dsDNA helicase)

Amethyst_36
(holliday junction resolvase)

Amethyst_37

Amethyst_38
(DNA primase)

Amethyst_39
(DNA primase)

Amethyst_40

Amethyst_41

Amethyst_42

Amethyst_43
(endonuclease)

Amethyst_44

Amethyst_45
(helix-turn-helix DNA binding domain protein)

Amethyst_46

Amethyst_47
(ribonucleotide reductase)

Amethyst_48

Amethyst_49

Amethyst_50
(helix-turn-helix DNA binding domain protein)

Amethyst_51
(integrase)

Amethyst_52

Amethyst_53
(thymidylate synthase)

Amethyst_54

Amethyst_55

Amethyst_56

Amethyst_57

Amethyst_58

Amethyst_59

Amethyst_60

Amethyst_61

Amethyst_62
(dNMP kinase)

Amethyst_63

Amethyst_64

Amethyst_65

Amethyst_66

Amethyst_67

Amethyst_68

Amethyst_69

Amethyst_70

Amethyst_71

Amethyst_72

Amethyst_73

Amethyst_74

Amethyst_75

Amethyst_76

Amethyst_77

Amethyst_78

Amethyst_79

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF766044
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload