Rhodococcus phage AngryOrchard

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Detailed Information for Phage AngryOrchard
Discovery Information
Isolation HostRhodococcus erythropolis RIA 643
Found ByMitchell Broadway, Sydnie Gates
Year Found2015
Location FoundSomerville, TN USA
Finding InstitutionUniversity of Louisiana at Monroe
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature34°C
GPS CoordinatesUnavailable
Discovery NotesThe phage was discovered under an apple tree, in Somerville, TN, during the beginning of August. The soil was extracted from the base of the tree closest to the trunk. The soil was cool and damp.
Naming NotesThe phage was found under an apple tree in an apple orchard. The name AngryOrchard stems from the thought of the orchard having angry phages, from the number of plagues.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 23, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2173
Genome length (bp)46597
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGTGAT
GC Content58.8%
Sequencing NotesThere was a polymorphism in the reads for this genome. About 40% of the reads at position 21686 had a "G" in them, instead of the final reported base of "A".
Fasta file available?Available upon entering GPS coordinates
Characterization
ClusterCA
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe AngryOrchard plagues started as cloudy, small plagues, but after a second spot test they became clear and remained small. The plagues appeared within 18-24 hours.
Has been Phamerated?Yes
Gene List

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AngryOrchard_1

AngryOrchard_2

AngryOrchard_3

AngryOrchard_6
(lysin)

AngryOrchard_7
(terminase large subunit)

AngryOrchard_8
(portal protein)

AngryOrchard_9
(capsid maturation protease)

AngryOrchard_10
(scaffolding protein)

AngryOrchard_11
(major capsid protein)

AngryOrchard_12
(head-to-tail connector protein)

AngryOrchard_13
(head-to-tail connector protein)

AngryOrchard_14
(head-to-tail connector protein)

AngryOrchard_15
(head-to-tail connector protein)

AngryOrchard_16
(head-to-tail connector protein)

AngryOrchard_17
(major tail protein)

AngryOrchard_18
(tail assembly chaperone)

AngryOrchard_19
(tail assembly chaperone)

AngryOrchard_20
(tapemeasure protein)

AngryOrchard_21
(minor tail protein)

AngryOrchard_22
(minor tail protein)

AngryOrchard_23
(minor tail protein)

AngryOrchard_24
(holin)

AngryOrchard_25

AngryOrchard_26
(minor tail protein)

AngryOrchard_27

AngryOrchard_28
(integrase)

AngryOrchard_29
(excise)

AngryOrchard_30
(membrane protein)

AngryOrchard_31
(deaminase)

AngryOrchard_32

AngryOrchard_33

AngryOrchard_34
(DNA polymerase I)

AngryOrchard_35
(HTH DNA binding protein)

AngryOrchard_36
(thymidylate synthase)

AngryOrchard_37

AngryOrchard_38

AngryOrchard_39
(ribonucleotide reductase)

AngryOrchard_40

AngryOrchard_41

AngryOrchard_42
(serine/threonine phosphatase)

AngryOrchard_43

AngryOrchard_44
(glutaredoxin)

AngryOrchard_45

AngryOrchard_46
(DNA primase)

AngryOrchard_47
(DNA primase)

AngryOrchard_48

AngryOrchard_49

AngryOrchard_50
(helicase)

AngryOrchard_51

AngryOrchard_52

AngryOrchard_53
(RecB-like exonuclease)

AngryOrchard_54

AngryOrchard_55
(HTH DNA-binding protein)

AngryOrchard_56

AngryOrchard_57

AngryOrchard_58

AngryOrchard_59

AngryOrchard_60

AngryOrchard_61

AngryOrchard_62
(antirestriction protein)

AngryOrchard_63

AngryOrchard_64

AngryOrchard_65

AngryOrchard_66

AngryOrchard_67

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKY549153
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 22
Pitt Freezer Box Grid# H5
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload