Mycobacterium phage Aroostook
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Detailed Information for Phage Aroostook
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLauren Rockwell
Year Found2015
Location FoundLongview , TX USA
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature37°C
GPS Coordinates32.467045 N, 94.724361 W Map
Discovery NotesI collected my environmental sample on campus at LeTourneau University on 9/17/15 at about 8:20 AM. The specific location of where I retrieved my sample was at the base of a bush beside a parking lot in front of a residence hall. The weather was 73°F and sunny. The soil was slightly moist, partly because it was at the base of the plant and also because the ground had dew on it.
Naming NotesUntil I moved to Texas four years ago, I had been living in Maine my entire life. I still consider myself a "Mainer" rather than a "Texan," and I always will. Because this place is very special and unique to me, I wanted to name my phage after it. Originally, I wanted to name my phage "Island Falls," the town in which I grew up. However, to give my phage a simpler, one-word name, I chose to name it Aroostook, the county in which Island Falls is located.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 4, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4385
Genome length (bp)41903
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCCATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterG
SubclusterG1
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF668268
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2*10^10 pfu/mL
Date of SEA Lysate Titering Oct 27, 2015
Pitt Freezer Box# 21
Pitt Freezer Box Grid# E5
Available Files
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload