Mycobacterium phage Astraea
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Detailed Information for Phage Astraea
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAmmu Vijay
Year Found2011
Location FoundRaleigh, NC USA
Finding InstitutionNorth Carolina State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates35.782178 N, 78.666092 W Map
Discovery NotesSoil from garden near Bagwell Hall. 74 degrees F. Collected on August 21, 2011 at 8:40pm. It was humid and drizzling outside.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 29, 2012
Sequencing FacilityDavid H. Murdock Research Institute
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage150
Genome length (bp)154872
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

TinyC (None)

Trilla23 (None)

Turret (None)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Breeniome (C1)

Bxz1 (C1)

Cali (C1)

Catera (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Delilah (C1)

Derek (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

FoxtrotP1 (C1)

FrayBell (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

HarrySpotter (C1)

HyRo (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LRRHood (C1)

Lukilu (C1)

Lysocool (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Napoleon13 (C1)

Nappy (C1)

Nicolasphaqe (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rizal (C1)

Roots515 (C1)

SandySandal (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zeenon (C1)

ZygoTaiga (C1)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Plaque NotesPlaques get to be 2 mm if left in incubator for 48 hours, but are 0.5 mm if left in incubator for 24 hours. Plaques are always clear and circular. Host range only includes types of M. smegmatis bacteria.
MorphotypeMyoviridae
Final annotation complete?Yes
Number of Genes232
Number of tRNAs31
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Astraea_1

Astraea_2

Astraea_3
(nucleotidyltransferase)

Astraea_4

Astraea_5

Astraea_6

Astraea_7

Astraea_8

Astraea_9

Astraea_10

Astraea_11

Astraea_12

Astraea_13

Astraea_14

Astraea_15

Astraea_16

Astraea_17

Astraea_18

Astraea_19

Astraea_20

Astraea_21

Astraea_22

Astraea_23

Astraea_24

Astraea_25

Astraea_26

Astraea_27

Astraea_28

Astraea_29

Astraea_30
(Band 7 protein)

Astraea_31

Astraea_32

Astraea_33

Astraea_34

Astraea_35

Astraea_36

Astraea_37

Astraea_38

Astraea_39

Astraea_40

Astraea_41

Astraea_42

Astraea_43

Astraea_44

Astraea_45

Astraea_46

Astraea_47

Astraea_48

Astraea_49

Astraea_50

Astraea_51

Astraea_52

Astraea_53

Astraea_54

Astraea_55

Astraea_56

Astraea_57

Astraea_58

Astraea_59

Astraea_60

Astraea_61

Astraea_62

Astraea_63

Astraea_64
(HTH-DNA binding protein)

Astraea_65

Astraea_66

Astraea_67

Astraea_68

Astraea_69

Astraea_70

Astraea_71

Astraea_72

Astraea_73

Astraea_74

Astraea_75

Astraea_76

Astraea_77

Astraea_78

Astraea_79

Astraea_80

Astraea_81
(ParB-like domain)

Astraea_86

Astraea_87

Astraea_88
(portal)

Astraea_89

Astraea_90

Astraea_91
(ThyX-like)

Astraea_92

Astraea_93
(phosphoesterase)

Astraea_94
(LysM domain)

Astraea_95

Astraea_96

Astraea_97
(major capsid protein)

Astraea_98

Astraea_99

Astraea_100

Astraea_101

Astraea_102

Astraea_103

Astraea_104

Astraea_105
(transmembrane domain)

Astraea_106

Astraea_107

Astraea_108

Astraea_109

Astraea_110

Astraea_111

Astraea_112

Astraea_113

Astraea_114

Astraea_115

Astraea_116

Astraea_117

Astraea_118

Astraea_119

Astraea_120

Astraea_121

Astraea_122

Astraea_123

Astraea_124

Astraea_125

Astraea_126
(tail assembly chaperone)

Astraea_127
(tail assembly chaperone)

Astraea_128
(tapemeasure)

Astraea_129

Astraea_130

Astraea_131

Astraea_132
(minor tail protein)

Astraea_133

Astraea_134
(minor tail protein)

Astraea_135

Astraea_136
(baseplate J)

Astraea_137
(HNH endonuclease)

Astraea_138

Astraea_139

Astraea_140

Astraea_141

Astraea_142

Astraea_143

Astraea_144

Astraea_145

Astraea_146

Astraea_147

Astraea_148

Astraea_151

Astraea_152
(Peptidyl-tRNA hydrolase)

Astraea_158

Astraea_167

Astraea_169
(D-alanine carboxypeptidase)

Astraea_170

Astraea_181

Astraea_182
(helicase)

Astraea_183

Astraea_184

Astraea_185

Astraea_186

Astraea_187

Astraea_188

Astraea_189

Astraea_190

Astraea_191

Astraea_192
(Aminotransferase)

Astraea_193

Astraea_194

Astraea_195

Astraea_196
(DnaC-like)

Astraea_197
(DnaB-like helicase)

Astraea_198

Astraea_199
(DNA Primase)

Astraea_200

Astraea_201
(DnaJ-like domain)

Astraea_202

Astraea_203
(DNA polymerase III alpha subunit)

Astraea_204
(RF-1 domain: peptide chain release factor.)

Astraea_205
(RecA-like)

Astraea_206

Astraea_207

Astraea_208
(HNH endonuclease domain)

Astraea_209
(RusA-like)

Astraea_210

Astraea_211

Astraea_212

Astraea_213

Astraea_214

Astraea_215

Astraea_216

Astraea_219

Astraea_220

Astraea_221

Astraea_222

Astraea_223

Astraea_224

Astraea_225

Astraea_226

Astraea_227
(Ro (TROVE domain, RNA binding))

Astraea_228

Astraea_229

Astraea_230

Astraea_231

Astraea_232

Astraea_233

Astraea_234

Astraea_235
(glycosyl transferase)

Astraea_236
(galactosyl transferase)

Astraea_237
(galactosyl transferase)

Astraea_238
(galactosyl transferase)

Astraea_239

Astraea_240
(Glycosyl transferse)

Astraea_241

Astraea_242

Astraea_243

Astraea_244

Astraea_245
(HNH endonuclease)

Astraea_246
(Lysin A)

Astraea_247
(holin)

Astraea_248
(Lysin B)

Astraea_249
(terminase)

Astraea_250

Astraea_251

Astraea_252

Astraea_253

Astraea_254
(polynucleotide kinase)

Astraea_255

Astraea_256

Astraea_257
(ser/thr kinase)

Astraea_258

Astraea_259

Astraea_260
(PurA-like)

Astraea_261

Astraea_262

Astraea_263

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKC691257
Refseq NumberNC_021349
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload