Mycobacterium phage Ayashe
Ayashe Plaque Picture
Ayashe EM Picture
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Detailed Information for Phage Ayashe
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByOlivia Phillips
Year Found2011
Location FoundRaleigh, NC USA
Finding InstitutionNorth Carolina State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates35.783 N, 78.666 W Map
Discovery NotesThe soil Ayashe was found in was dark brown and moist because it was collected right before it stormed. The soil was just under some mulch.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaques were consistently clear and circular. They were about 2.5 mm wide.

Also did a host range test for ayashe, using M.Smegmatis mc2 155, M. smegmatis trevisan, M. smegmatis MH, M. austroafricanum, M. vanbaalemi, and M.spp. ELW1. The M. spp. ELW1 did not grow in the plate so we disregarded this bacterium. However, Ayashe lysed M. smegmatis mc2 155 (clear plaque), M. Smegmatis MH (clear plaques), and M. smegmatis trevisan (trubid plaques).
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2011NCSUayasheOMP
SEA Lysate Titer 3e13
Date of SEA Lysate Titering Sep 28, 2011
Pitt Freezer Box# 26
Pitt Freezer Box Grid# 31
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload