Mycobacterium phage BQuat
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Detailed Information for Phage BQuat
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHillary Sigale and Sarah Jacobs
Year Found2010
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates38.645638 N, 90.311388 W Map
Discovery NotesSoil moist but not saturated, soil from base of compost bins in Burning Kumquat organic garden on Washington University's campus, air temperature was approximately 29C, sample taken from top soil (about 1-2 cm deep).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 18, 2012
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage136
Sanger Finishing Reactions3
Genome length (bp)41893
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCCATGGCAT
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterG
SubclusterG1
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 5/15/2018)
Plaque NotesPlaques are clear and circular with sharp distinct edges. Plaque diameter is approximately .10-.15 cm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload