Mycobacterium phage Bartholomew
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Detailed Information for Phage Bartholomew
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMegan Fallert, Lucinda Krenzke, Dezerene Thompson
Year Found2014
Location FoundKenosha, WI USA
Finding InstitutionCarthage College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.619444 N, 87.821389 W Map
Discovery NotesThe soil sample was taken at night from the dirt behind a shrub outside Madrigrano Residence Hall. The soil was taken at a depth of one inch and the temperature was 68 degrees F.
Naming NotesBecause our phage's plaques have a distinct halo, we named it after the Catholic saint Bartholomew.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 18, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage150
Genome length (bp)46484
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceCCCGCCCCCCGA
GC Content67.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterP
SubclusterP1
Other Cluster Members
Plaque NotesBartholomew is temperate and has two distinct morphologies. Approximately 85% of the plaques on a given plate display a morphology that is 2mm in size and has a distinct halo with a clear center. The other morphology is about 1mm in size and appears as a pinprick.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Bartholomew_1
(terminase small subunit)

Bartholomew_2
(terminase large subunit)

Bartholomew_3
(portal protein)

Bartholomew_4
(capsid maturation protease)

Bartholomew_5
(scaffolding protein)

Bartholomew_6
(major capsid protein)

Bartholomew_7

Bartholomew_8
(head-to-tail connector complex protein)

Bartholomew_9
(head-to-tail connector complex protein)

Bartholomew_10

Bartholomew_11

Bartholomew_12

Bartholomew_13
(head-to-tail connector complex protein)

Bartholomew_14
(major tail protein)

Bartholomew_15
(tail assembly chaperone)

Bartholomew_16
(tail assembly chaperone)

Bartholomew_17
(tape measure protein)

Bartholomew_18
(minor tail protein)

Bartholomew_19
(minor tail protein)

Bartholomew_20
(minor tail protein)

Bartholomew_21
(minor tail protein)

Bartholomew_22
(minor tail protein)

Bartholomew_23

Bartholomew_24

Bartholomew_25
(lysin A)

Bartholomew_26
(lysin B)

Bartholomew_27
(holin)

Bartholomew_28

Bartholomew_29
(integrase)

Bartholomew_30
(immunity repressor)

Bartholomew_31

Bartholomew_32

Bartholomew_33
(antirepressor)

Bartholomew_34

Bartholomew_35

Bartholomew_36

Bartholomew_37

Bartholomew_38

Bartholomew_39

Bartholomew_40

Bartholomew_41

Bartholomew_42

Bartholomew_43

Bartholomew_44

Bartholomew_45
(exonuclease)

Bartholomew_46
(ssDNA binding protein)

Bartholomew_47

Bartholomew_48
(DNA translocase)

Bartholomew_49
(resolvase)

Bartholomew_50

Bartholomew_51

Bartholomew_52

Bartholomew_53
(DNA methylase)

Bartholomew_54

Bartholomew_55
(HTH DNA binding protein)

Bartholomew_56

Bartholomew_57
(DNA methylase)

Bartholomew_58
(HTH DNA binding protein)

Bartholomew_59

Bartholomew_60

Bartholomew_61
(HTH DNA binding domain protein)

Bartholomew_62

Bartholomew_63

Bartholomew_64

Bartholomew_65

Bartholomew_66

Bartholomew_67

Bartholomew_68

Bartholomew_69

Bartholomew_70

Bartholomew_71

Bartholomew_72

Bartholomew_73

Bartholomew_74

Bartholomew_75

Bartholomew_76

Bartholomew_77
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140399
Refseq NumberNone yet
Available Files
EM PictureDownload
GenBank File for PhameratorDownload