Gordonia phage Bjanes7
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Detailed Information for Phage Bjanes7
Discovery Information
Isolation HostGordonia terrae 3612
Found ByLinn Bjanes
Year Found2016
Location FoundPittsburgh, PA United States of America
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.445 N, 79.953333 W Map
Discovery NotesIdentified area with sprouting new plants in a flower bed near the Cathedral of Learning. There was a fresh layer of snow on top of the dirt so I brushed away the snow and dug a few inches underneath the top soil.

I dug roughly 3 inches into the earth about 2 inches from the nearest plant
Collected 1 spoonful of dirt (the ground was frozen)
Placed the soil sample into the plastic bag and sealed the bag with the zip closures
Recorded the GPS coordinates using the compass app (see below)
After roughly 5 minutes of transport, the soil sample was put into a conical tube for enrichment


Additional Notes:

GPS coordinates of the soil collection location: 40˚26’42” N 79˚57’12” W
Weather conditions at the time of the collection: strong winds, overcast with snow showers, 34 degrees F
Weather conditions in the last 24 hours: frequent snow showers, gusting winds and mostly cloudy; high 20s during the day and low 10s during the night
Soil conditions: soil was moist and mixed in with grass, mulch and bits of snow and ice.
Naming NotesLast name: Bjanes and 7 because it was in sample #7 that the phage was discovered.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 30, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2827
Genome length (bp)46042
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTACCAGGGGGA
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ2
Other Cluster Members
Plaque NotesThe plaque morphologies are both small, clear plaques and large, turbid plaques. The large ones have haloes, hazy outer edges and a tiny, clear dot in the center.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Bjanes7_1
(terminase, small subunit)

Bjanes7_2
(terminase, large subunit)

Bjanes7_3
(portal protein)

Bjanes7_4
(capsid maturation protease)

Bjanes7_5
(scaffolding protein)

Bjanes7_6
(major capsid protein)

Bjanes7_7
(head-to-tail connector protein)

Bjanes7_8
(head-to-tail connector protein)

Bjanes7_9
(head-to-tail connector protein)

Bjanes7_10
(head-to-tail connector protein)

Bjanes7_11
(head-to-tail connector protein)

Bjanes7_12
(major tail subunit)

Bjanes7_13
(tail assembly chaperone)

Bjanes7_14
(tail assembly chaperone)

Bjanes7_15
(tape measure protein)

Bjanes7_16
(minor tail protein)

Bjanes7_17
(minor tail protein)

Bjanes7_18
(lysin A)

Bjanes7_19

Bjanes7_20

Bjanes7_21

Bjanes7_22

Bjanes7_23

Bjanes7_24
(lysin B)

Bjanes7_25
(minor tail protein)

Bjanes7_26

Bjanes7_27

Bjanes7_28
(minor tail protein)

Bjanes7_29

Bjanes7_30

Bjanes7_31

Bjanes7_32

Bjanes7_33

Bjanes7_34
(integrase (Y-int))

Bjanes7_35

Bjanes7_36

Bjanes7_37
(HTH DNA-binding protein)

Bjanes7_38

Bjanes7_39

Bjanes7_40

Bjanes7_41
(immunity repressor)

Bjanes7_42
(HTH DNA binding domain protein)

Bjanes7_43

Bjanes7_44
(DNA polymerase III, beta subunit)

Bjanes7_45
(DNA polymerase III, beta subunit)

Bjanes7_46

Bjanes7_47

Bjanes7_48

Bjanes7_49

Bjanes7_50
(RecE Exonuclease)

Bjanes7_51
(RecT ssDNA binding domain)

Bjanes7_52

Bjanes7_53

Bjanes7_54

Bjanes7_55

Bjanes7_56

Bjanes7_57

Bjanes7_58
(DNA methylase)

Bjanes7_59

Bjanes7_60

Bjanes7_61

Bjanes7_62

Bjanes7_63
(HTH DNA binding domain protein)

Bjanes7_64

Bjanes7_65
(RusA resolvase (endonuclease))

Bjanes7_66

Bjanes7_67
(HTH DNA-binding domain protein)

Bjanes7_68

Bjanes7_69

Bjanes7_70

Bjanes7_71

Bjanes7_72
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload