Gordonia phage Boneham

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Detailed Information for Phage Boneham
Discovery Information
Isolation HostGordonia terrae 3612
Found ByBethany Lindsey
Year Found2016
Location FoundPittsburgh, PA United States of America
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Discovery NotesThis phage was found on 1/28/16 in a flower bed outside of the Cathedral of Learning the University of Pittsburgh campus. No plants were currently growing within the bed. The temperature during discovery was 43 degrees celcius and had been cloudy the past 24 hours. There were minimal snow showers around the time of harvesting and there was no snow cover in the flowerbed.
Naming NotesNamed after 2004 "Survivor" contestant Rupert Boneham. This phage survived mass contamination of an enrichment so I figured it was appropriate to name it after a fan favorite on the television show where surviving is the means of winning.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 6, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1822
Genome length (bp)77497
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length191 bp
GC Content58.9%
Fasta file available?Available upon entering GPS coordinates
Characterization
ClusterCS
SubclusterCS2
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaques are ~1mm in diameter and are clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Boneham_1

Boneham_2

Boneham_3

Boneham_4

Boneham_5

Boneham_6

Boneham_7

Boneham_8
(DNA primase)

Boneham_9
(terminase small subunit)

Boneham_10
(terminase large subunit)

Boneham_11
(portal protein)

Boneham_12

Boneham_13

Boneham_14
(capsid maturation protease)

Boneham_15
(scaffolding protein)

Boneham_16
(major capsid protein)

Boneham_17
(head-to-tail connector protein)

Boneham_18
(head-to-tail connector protein)

Boneham_19
(head-to-tail connector protein)

Boneham_20
(head-to-tail connector protein)

Boneham_21
(head-to-tail connector protein)

Boneham_22
(head-to-tail connector protein)

Boneham_23
(major tail protein)

Boneham_25
(tail assembly chaperone)

Boneham_26
(tail assembly chaperone)

Boneham_27
(tape measure protein)

Boneham_28
(minor tail protein)

Boneham_29
(minor tail protein)

Boneham_30
(minor tail protein)

Boneham_31

Boneham_32

Boneham_33

Boneham_34

Boneham_35

Boneham_36

Boneham_37

Boneham_38
(lysin A, N-acetyl-BetaD-muramidase domain)

Boneham_39
(lysin A, peptidase domain)

Boneham_40

Boneham_41

Boneham_42

Boneham_43

Boneham_44
(endonuclease/helicase)

Boneham_45

Boneham_46

Boneham_47

Boneham_48

Boneham_49

Boneham_50
(DNA methylase)

Boneham_51

Boneham_52

Boneham_53
(lysin B)

Boneham_54
(nucleotide pyrophosphohydrolase)

Boneham_55

Boneham_56

Boneham_57

Boneham_58

Boneham_59
(DNA polymerase III sliding clamp (beta))

Boneham_60
(exonuclease)

Boneham_61
(helix turn helix DNA-binding protein)

Boneham_62

Boneham_63
(DNA helicase)

Boneham_64

Boneham_65
(helix turn helix DNA-binding protein)

Boneham_66

Boneham_67
(helix turn helix DNA-binding protein)

Boneham_68

Boneham_69

Boneham_70

Boneham_71

Boneham_72

Boneham_73

Boneham_74

Boneham_75

Boneham_76

Boneham_77

Boneham_78

Boneham_79

Boneham_80

Boneham_81

Boneham_82

Boneham_83

Boneham_84

Boneham_85
(HNH endonuclease)

Boneham_86

Boneham_87

Boneham_88

Boneham_89

Boneham_90
(cobalamin biosynthesis protein)

Boneham_91
(AAA-ATPase)

Boneham_92

Boneham_93

Boneham_94

Boneham_95

Boneham_96

Boneham_97

Boneham_98

Boneham_99

Boneham_100

Boneham_101

Boneham_102

Boneham_103

Boneham_104

Boneham_105

Boneham_106

Boneham_107

Boneham_108

Boneham_109

Boneham_110

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG757155
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 3
Pitt Freezer Box Grid# C6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload