The Actinobacteriophage Database

Mycobacterium phage Bongo

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Detailed Information for Phage Bongo
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Han Yuan and James Wherley
Year Found	2009
Location Found	St Louis, MO USA
Finding Institution	Washington University in St. Louis
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	38.644208 N, 90.314927 W Map
Discovery Notes	Washington University Campus under a tree near basketball courts. Soil depth: 3cm. Temperature 24 degree Celsius.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	May 23, 2011
Genome length (bp)	80228
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	ACCTCCTGCAA
GC Content	61.6%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	M
Subcluster	M1
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Auspice (M1) Bongo (M1) Bricole (M1) Diminimus (M1) Dulcita (M1) Glaske16 (M1) IPhane7 (M1) LilhomieP (M1) PegLeg (M1) Reindeer (M1) Skinny (M1) SlimJimmy (M1) TyDawg (M1) Aziz (M2) Estes (M2) GardenSalsa (M2) GenevaB15 (M2) MrMagoo (M2) Rey (M2) Nanosmite (M3)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	1mm/ clear/ Incubation 24-48 hours at 37 degrees Celsius.
Morphotype	Siphoviridae
Number of Genes	132
Number of tRNAs	19
Number of tmRNAs	1
Has been Phamerated?	Yes
Gene List	Click to View Bongo_1 Bongo_2 Bongo_3 (Lsr2-like DNA bridging protein) Bongo_4 Bongo_5 Bongo_6 Bongo_7 Bongo_8 Bongo_9 Bongo_10 Bongo_11 Bongo_12 Bongo_13 Bongo_14 (terminase large subunit) Bongo_15 (portal protein) Bongo_16 (MuF-like minor capsid protein) Bongo_17 (scaffolding protein) Bongo_18 (major capsid protein) Bongo_19 (head-to-tail adaptor) Bongo_20 (head-to-tail stopper) Bongo_21 Bongo_22 (tail terminator) Bongo_23 (major tail protein) Bongo_25 (tail assembly chaperone) Bongo_24 (tail assembly chaperone) Bongo_26 (tape measure protein) Bongo_27 (minor tail protein) Bongo_28 (minor tail protein) Bongo_29 (minor tail protein) Bongo_30 (minor tail protein) Bongo_31 Bongo_32 Bongo_33 Bongo_34 Bongo_35 (lysin A) Bongo_36 Bongo_37 (holin) Bongo_38 (lysin B) Bongo_39 (NrdH-like glutaredoxin) Bongo_40 Bongo_41 Bongo_42 Bongo_43 Bongo_44 Bongo_45 Bongo_46 Bongo_47 (nucleotidyl transferase) Bongo_48 Bongo_49 Bongo_50 Bongo_51 Bongo_52 Bongo_53 Bongo_54 (ERF family ssDNA binding protein) Bongo_55 Bongo_56 Bongo_57 Bongo_58 (ClpP-like protease) Bongo_59 Bongo_60 Bongo_61 Bongo_62 Bongo_63 Bongo_64 Bongo_65 Bongo_66 Bongo_67 (ssDNA binding protein) Bongo_68 Bongo_69 (DnaB-like dsDNA helicase) Bongo_70 Bongo_71 Bongo_72 (WhiB family transcription factor) Bongo_73 Bongo_74 Bongo_75 Bongo_76 Bongo_77 (endonuclease VII) Bongo_78 Bongo_79 (DnaQ-like DNA polymerase III subunit) Bongo_80 Bongo_81 Bongo_82 Bongo_83 (PTPc tyrosine phosphatase) Bongo_84 Bongo_85 Bongo_86 Bongo_87 Bongo_88 Bongo_89 (HNH endonuclease) Bongo_90 Bongo_101 Bongo_105 Bongo_107 (ribonucleotide reductase) Bongo_115 (RtcB-like RNA ligase) Bongo_116 (band-7-like membrane protein) Bongo_117 (MazG-like nucleotide pyrophosphohydrolase) Bongo_118 Bongo_119 Bongo_120 Bongo_121 Bongo_122 Bongo_123 (DprA-like DNA processing chain A) Bongo_124 Bongo_125 (metallophosphoesterase) Bongo_126 Bongo_127 Bongo_128 Bongo_129 Bongo_130 (serine integrase) Bongo_131 Bongo_132 Bongo_133 Bongo_134 Bongo_135 Bongo_136 Bongo_137 Bongo_138 Bongo_139 Bongo_140 Bongo_141 Bongo_142 Bongo_143 Bongo_144 Bongo_145 Bongo_146 Bongo_147 Bongo_148 Bongo_149 Bongo_150 Bongo_151 Bongo_152 Bongo_153
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	JN699628
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	WUSL
SEA Lysate Titer	8.4e8
Pitt Freezer Box#	1
Pitt Freezer Box Grid#	C7
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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