Mycobacterium phage Bonnie2013
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| Detailed Information for Phage Bonnie2013 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Christine Peters |
| Year Found | 2013 |
| Location Found | San Diego , CA USA |
| Finding Institution | University of California, San Diego |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.876831 N, 117.241043 W Map |
| Discovery Notes | I obtained my phage from soil under a tree, the soil was in a shaded area, and it felt moist but was not damp or wet to the touch. The soil was surrounded by a grassy area and was a couple of feet from a sidewalk with high foot traffic however the area where the soil actually was has very low foot traffic. The soil came from approximately 1 cm deep. The temperature of the air was 81 degrees Fahrenheit and the air was not humid. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques were approximately 1-4mm in diameter. When first performing the plaque purification through the streaking method my plaques were smaller, being closer to 1-2mm and were clear. When I performed the 1 plate and 10 plate lysates however, my plaques changed and got bigger, being closer to 4mm and were haloed, resembling a bulls eye, they had a clear middle and a turbid circle surrounding the clear middle. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 1 |
| Pitt Freezer Box Grid# | F5 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |