Mycobacterium phage Bromden
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Detailed Information for Phage Bromden
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHannah E Cizauskas
Year Found2017
Location FoundWestland, MI United States
Finding InstitutionHope College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates42.2896 N, 83.4289 W Map
Discovery NotesSlightly moist soil from a pumpkin patch that was composted with grass clippings.
Naming NotesThis phage is named after the main character in One Flew Over the Cuckoo's Nest, written by Ken Kesey. Chief Bromden is the only character that escaped the insane asylum by checking himself out. The other characters either remained in the asylum or died in there. He was the one that got out, much like my phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 8, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1973
Genome length (bp)70183
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content58.2%
Sequencing NotesDespite a high level of coverage, the overhang was not present in the sequencing reads, and so it was inferred from highly similar genomes. Sanger sequencing will be attempted to cover this region.
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL4
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesPlaques are circular, clear, and .5mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Bromden_1
(terminase small subunit)

Bromden_2

Bromden_3

Bromden_4
(terminase large subunit)

Bromden_5
(portal protein)

Bromden_6
(capsid maturation protease)

Bromden_7
(scaffolding protein)

Bromden_8
(major capsid protein)

Bromden_9
(head-to-tail connector complex protein)

Bromden_10
(head-to-tail connector complex protein)

Bromden_11
(head-to-tail connector complex protein)

Bromden_12
(head-to-tail connector complex protein)

Bromden_13
(HNH endonuclease)

Bromden_14
(major tail protein)

Bromden_15
(tail assembly chaperone)

Bromden_16
(tail assembly chaperone)

Bromden_17
(tape measure protein)

Bromden_18
(minor tail protein)

Bromden_19
(minor tail protein)

Bromden_20
(minor tail protein)

Bromden_21
(minor tail protein)

Bromden_22

Bromden_23

Bromden_24

Bromden_25
(hydrolase)

Bromden_26

Bromden_27
(lysin A)

Bromden_28
(lysin B)

Bromden_29
(holin)

Bromden_30
(holin)

Bromden_31

Bromden_32
(glutaredoxin)

Bromden_33

Bromden_34
(nucleotidyltransferase)

Bromden_35
(DNA bridging protein)

Bromden_36

Bromden_37
(integrase)

Bromden_38

Bromden_39
(immunity repressor)

Bromden_40
(Cro protein)

Bromden_41
(excise)

Bromden_42

Bromden_43

Bromden_44

Bromden_45

Bromden_46

Bromden_47
(metallophosphoesterase)

Bromden_48

Bromden_49

Bromden_50
(band-7-like membrane protein)

Bromden_51

Bromden_52

Bromden_53

Bromden_54
(RNA binding protein)

Bromden_55

Bromden_56

Bromden_57

Bromden_58

Bromden_59

Bromden_60
(exonuclease)

Bromden_61
(endonuclease VII)

Bromden_62

Bromden_63
(ERF family ssDNA binding protein)

Bromden_64

Bromden_65

Bromden_66

Bromden_67
(HNH endonuclease)

Bromden_68

Bromden_69

Bromden_70
(dsDNA helicase)

Bromden_71
(WhiB family transcription factor)

Bromden_72

Bromden_73

Bromden_74
(phosphoribosyl transferase)

Bromden_75
(nicotinate ribosyltransferase)

Bromden_76
(polynucleotide kinase)

Bromden_77

Bromden_78
(resolvase)

Bromden_79
(WhiB family transcription factor)

Bromden_80
(WhiB family transcription factor)

Bromden_81

Bromden_82

Bromden_83

Bromden_84
(ssDNA binding protein)

Bromden_85

Bromden_86

Bromden_87

Bromden_88
(RNA ligase)

Bromden_89
(HNH endonuclease)

Bromden_90
(protease)

Bromden_91

Bromden_92

Bromden_93

Bromden_94

Bromden_95

Bromden_96
(HNH endonuclease)

Bromden_97

Bromden_98

Bromden_99
(DNA polymerase III subunit)

Bromden_100

Bromden_101

Bromden_102

Bromden_103
(HNH endonuclease)

Bromden_104
(endonuclease VII)

Bromden_105

Bromden_106

Bromden_107

Bromden_108

Bromden_109

Bromden_112

Bromden_113

Bromden_117

Bromden_125

Bromden_126

Bromden_127

Bromden_128

Bromden_130

Bromden_131

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH576973
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 50
Pitt Freezer Box Grid# D2
Available Files
GenBank File for PhameratorDownload