Gordonia phage Bubbles11
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Detailed Information for Phage Bubbles11
Discovery Information
Isolation HostGordonia terrae 3612
Found ByAnjana Ettyreddy
Year Found2018
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature23°C
GPS Coordinates40.4426 N, 79.9567 W Map
Discovery NotesI collected the soil sample in the flower beds of the Litchfield Towers Patio at the University of Pittsburgh. The soil was on the dryer side but not too dry. The sample I collected was just a little under the top layer of the soil. And I think it had some organic matter in it from the decaying flower petals that would occasionally fall in. It was 73 degrees outside and the humidity was 91%.
Naming NotesThere is no specific reason why I chose the name I did. Bubbles is a name I would give to a pet and 11 is my favorite number.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Lysogeny NotesDo not know lysogen for sure, attempted to do the Lysogen Testing protocol but did not get a chance to finish, however a mesa was formed so it is most likely a temperate phage but cannot confirm until protocol is finished.
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe morphology of the phage is unique in the sense that it does not produce plaque sizes that are all the same. It produced 3 different sizes of plaques which was consistent over multiple round of purification. The largest pf the plaques are about 4mm, the medium sized plaques are around 2.5mm and the smallest are about 1mm. The individually plaques are all clear in the center with a cloudiness around the edges, they have haloes.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2E10
Pitt Freezer Box# 89
Pitt Freezer Box Grid# H7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload