Mycobacterium phage Calico
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| Detailed Information for Phage Calico | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | YY445 |
| Found By | Janine Yang |
| Year Found | 2015 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Drexel University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.9492 N, 75.1982 W Map |
| Discovery Notes | Time and Date collected: 22 September 2015 at 3:32PM Weather and Temperature: 72F, cloudy skies Sample's depth in soil: 1-3 inch Location: Near University of Pennsylvania's Biopond (Biopond, Philadelphia PA 19104); moist firm soil next to natural green overgrowth and foliage with manmade small lake approximately 10 feet away; separated from pond with concrete pathway and pond's rocky lining |
| Naming Notes | I chose Calico because my phage was isolated and purified from a very diverse set of phages with different diameters, and it was very difficult to isolate because there would always be other plaque diameters present. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaque diameter from original soil sample initially varied from 1mm to 4mm. After purification and isolation, plaques were average 3mm diameter with clear morphology and clean edges. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 10.5 x 10^10 pfu/mL |
| Date of SEA Lysate Titering | Nov 24, 2015 |
| Pitt Freezer Box# | 25 |
| Pitt Freezer Box Grid# | E3 |