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Gordonia phage CaptainKirk2
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Detailed Information for Phage CaptainKirk2
Discovery Information
Isolation HostGordonia terrae 3612
Found ByCharu Arora
Year Found2015
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.44574 N, 79.95372 W Map
Discovery NotesThe soil sample was collected near a flower garden, it was cloudy and 70 degrees. I adopted this from Alison Hoke's soil sample and original plate.
Naming NotesI chose to name this star trek, as Ali and I had decided together to name hers Captain Janeway following the idea of mystery presented by the phages and the urge I have to explore and understand my phage, similar to Captain Kirk.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 27, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3241
Genome length (bp)47898
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGCCGGTGA
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCV
Subcluster--
Other Cluster Members
Plaque NotesThere were two phages from the sample Ali collected. She collected the smaller plaque and named it (Captain Janeway) and I collected the larger plaque, which became my phage. Originally I thought I had 2 morphologies on my plaque, but I didn't after multiple tests.
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes79
Has been Phamerated?Yes
Gene List

Click to View

CaptainKirk2_1
(terminase, small subunit)

CaptainKirk2_2
(terminase, large subunit)

CaptainKirk2_3
(portal protein)

CaptainKirk2_4
(capsid maturation protease)

CaptainKirk2_5
(scaffolding protein)

CaptainKirk2_6
(major capsid protein)

CaptainKirk2_7
(head-to-tail connector protein)

CaptainKirk2_8
(head-to-tail connector protein)

CaptainKirk2_9
(head-to-tail connector protein)

CaptainKirk2_10

CaptainKirk2_11
(head-to-tail connector protein)

CaptainKirk2_12
(head-to-tail connector protein)

CaptainKirk2_13
(head-to-tail connector protein)

CaptainKirk2_14
(major tail protein)

CaptainKirk2_15
(tail assembly chaperone)

CaptainKirk2_16
(tail assembly chaperone)

CaptainKirk2_17
(tape measure protein)

CaptainKirk2_18
(minor tail protein)

CaptainKirk2_19
(minor tail protein)

CaptainKirk2_20
(lysin A, L-Ala-D-Glu peptidase domain)

CaptainKirk2_21
(lysin A, glycosyl hydrolase domain)

CaptainKirk2_22
(holin)

CaptainKirk2_23
(holin)

CaptainKirk2_24
(holin)

CaptainKirk2_25
(Lysin B)

CaptainKirk2_26

CaptainKirk2_27
(minor tail protein)

CaptainKirk2_28
(minor tail protein)

CaptainKirk2_29

CaptainKirk2_30
(minor tail protein)

CaptainKirk2_31

CaptainKirk2_32

CaptainKirk2_33

CaptainKirk2_34

CaptainKirk2_35
(antitoxin, toxin-antitoxin system)

CaptainKirk2_36
(toxin, toxin-antitoxin system)

CaptainKirk2_37

CaptainKirk2_38

CaptainKirk2_39

CaptainKirk2_40
(Integrase (Y-int))

CaptainKirk2_41

CaptainKirk2_42
(immunity repressor)

CaptainKirk2_43

CaptainKirk2_44

CaptainKirk2_45

CaptainKirk2_46

CaptainKirk2_47

CaptainKirk2_48

CaptainKirk2_49

CaptainKirk2_50

CaptainKirk2_51
(HTH DNA binding protein)

CaptainKirk2_52

CaptainKirk2_53

CaptainKirk2_54

CaptainKirk2_55

CaptainKirk2_56

CaptainKirk2_57
(DnaQ exonuclease)

CaptainKirk2_58
(RecE exonuclease)

CaptainKirk2_59
(RecT ssDNA binding protein)

CaptainKirk2_60

CaptainKirk2_61

CaptainKirk2_62

CaptainKirk2_63

CaptainKirk2_64

CaptainKirk2_65

CaptainKirk2_66

CaptainKirk2_67

CaptainKirk2_68

CaptainKirk2_69
(HNH endonuclease)

CaptainKirk2_70

CaptainKirk2_71

CaptainKirk2_72

CaptainKirk2_73

CaptainKirk2_74

CaptainKirk2_75
(RuvC resolvase)

CaptainKirk2_76

CaptainKirk2_77

CaptainKirk2_78

CaptainKirk2_79
(HNH endonuclease)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX557274
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Final DNAMaster FileDownload