Mycobacterium phage CharlieB
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Detailed Information for Phage CharlieB
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesCharliebrown
Found ByBrigham Wright
Year Found2009
Location FoundProvo, UT USA
Finding InstitutionBrigham Young University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates40.246244 N, 111.64965 W Map
Discovery NotesDate: 17 SEP 2009
Time: 1:30PM
Temperature: 79°F
Site: 40 feet West of Widtsoe Building; grove of trees, just north of last tree.
Sample: Mulched soil; taken 5cm from surface of soil. Found about forty feet west of the Widtsoe Building in a small patch of dirt near the bottom of a tree.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 12, 2012
Sequencing FacilityBYU DNA Sequencing Center
Shotgun Sequencing Method454 Pyrosequencing
Genome length (bp)155886
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

TinyC (None)

Trilla23 (None)

Turret (None)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Doodle1 (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

HarrySpotter (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Jessibeth14 (C1)

JustHall (C1)

Kamryn (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Lionheart (C1)

Littleton (C1)

Loanshark (C1)

LRRHood (C1)

Lukilu (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

MrsHay (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

OaklaforG (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

SandySandal (C1)

Sauce (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Annotation StatusIn GenBank
Plaque NotesPlaque size: 2 mm
Plaque turbidity:
Plaque shape: circular
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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CharlieB_1

CharlieB_2

CharlieB_3

CharlieB_4

CharlieB_5

CharlieB_6

CharlieB_7

CharlieB_8

CharlieB_9

CharlieB_10

CharlieB_11

CharlieB_12

CharlieB_13

CharlieB_14

CharlieB_15

CharlieB_16

CharlieB_17

CharlieB_18

CharlieB_19

CharlieB_20

CharlieB_21

CharlieB_22

CharlieB_23

CharlieB_24

CharlieB_25

CharlieB_26

CharlieB_27

CharlieB_28

CharlieB_29
(membrane protein, Band-7 like)

CharlieB_30

CharlieB_31

CharlieB_32

CharlieB_33

CharlieB_34

CharlieB_35

CharlieB_36

CharlieB_37

CharlieB_38

CharlieB_39

CharlieB_40

CharlieB_41

CharlieB_42

CharlieB_43

CharlieB_44

CharlieB_45

CharlieB_46

CharlieB_47

CharlieB_48

CharlieB_49

CharlieB_50

CharlieB_51

CharlieB_52

CharlieB_53

CharlieB_54

CharlieB_55

CharlieB_56

CharlieB_57

CharlieB_58

CharlieB_59

CharlieB_60
(Helix-Turn-Helix DNA binding domain)

CharlieB_61

CharlieB_62

CharlieB_63

CharlieB_64

CharlieB_65

CharlieB_66

CharlieB_67

CharlieB_68

CharlieB_69

CharlieB_70

CharlieB_71

CharlieB_72

CharlieB_73

CharlieB_74

CharlieB_75

CharlieB_76

CharlieB_77

CharlieB_78

CharlieB_79

CharlieB_83

CharlieB_84

CharlieB_85
(portal protein)

CharlieB_86

CharlieB_87

CharlieB_88

CharlieB_89

CharlieB_90
(RNA ligase)

CharlieB_91

CharlieB_92

CharlieB_93

CharlieB_94

CharlieB_95
(major capsid protein)

CharlieB_96
(endonuclease)

CharlieB_97

CharlieB_98

CharlieB_99

CharlieB_100

CharlieB_101

CharlieB_102

CharlieB_103

CharlieB_104

CharlieB_105

CharlieB_106

CharlieB_107

CharlieB_108

CharlieB_109

CharlieB_110

CharlieB_111

CharlieB_112

CharlieB_113

CharlieB_114

CharlieB_115

CharlieB_116

CharlieB_117

CharlieB_118

CharlieB_119

CharlieB_120

CharlieB_121

CharlieB_122

CharlieB_123

CharlieB_124

CharlieB_125

CharlieB_126

CharlieB_127
(tail assembly chaperone)

CharlieB_128
(tail assembly chaperone)

CharlieB_129
(tape measure protein)

CharlieB_130

CharlieB_131

CharlieB_132

CharlieB_133

CharlieB_134

CharlieB_135

CharlieB_136

CharlieB_137
(baseplate J protein)

CharlieB_138
(HNH endonuclease)

CharlieB_139

CharlieB_140

CharlieB_141

CharlieB_142

CharlieB_143
(minor tail protein)

CharlieB_144

CharlieB_145

CharlieB_146

CharlieB_147

CharlieB_148

CharlieB_149

CharlieB_150

CharlieB_152

CharlieB_153
(peptidyl tRNA hydrolase)

CharlieB_169
(minor tail protein, D-ala-D-ala carboxypeptidase)

CharlieB_170

CharlieB_180

CharlieB_181
(helicase)

CharlieB_182

CharlieB_183

CharlieB_184

CharlieB_185

CharlieB_186

CharlieB_187

CharlieB_188

CharlieB_189

CharlieB_190

CharlieB_191
(thymidylate kinase)

CharlieB_192
(aminotransferase)

CharlieB_193

CharlieB_194

CharlieB_195

CharlieB_196
(DnaC-like helicase loader)

CharlieB_197
(DnaB-like dsDNA helicase)

CharlieB_198

CharlieB_199
(DNA primase)

CharlieB_200

CharlieB_201
(chaperonin, DnaJ-like)

CharlieB_202

CharlieB_203
(DNA polymerase III alpha subunit)

CharlieB_204
(RF-1 peptide chain release factor)

CharlieB_205
(RecA-like DNA recombinase)

CharlieB_206

CharlieB_207

CharlieB_208
(HNH endonuclease)

CharlieB_209
(RusA-like resolvase)

CharlieB_210

CharlieB_211

CharlieB_212

CharlieB_213

CharlieB_214

CharlieB_215

CharlieB_216

CharlieB_219

CharlieB_220

CharlieB_221

CharlieB_222

CharlieB_223

CharlieB_224

CharlieB_225

CharlieB_226

CharlieB_227
(Ro-like RNA binding protein)

CharlieB_228

CharlieB_229

CharlieB_230

CharlieB_231

CharlieB_232

CharlieB_233

CharlieB_234

CharlieB_235

CharlieB_236

CharlieB_237
(glycosyltransferase)

CharlieB_238
(galactosyltransferase)

CharlieB_239

CharlieB_240

CharlieB_241

CharlieB_242

CharlieB_243

CharlieB_244

CharlieB_245
(lysin A)

CharlieB_246
(holin)

CharlieB_247
(lysin B)

CharlieB_248
(terminase)

CharlieB_249

CharlieB_250

CharlieB_251

CharlieB_252

CharlieB_253
(polynucleotide kinase)

CharlieB_254

CharlieB_255

CharlieB_256
(serine/threonine kinase)

CharlieB_257
(HNH endonuclease)

CharlieB_258

CharlieB_259
(adenylosuccinate synthetase, PurA-like)

CharlieB_260

CharlieB_261

CharlieB_262

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH727543
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2009BRYOcharliebBW
SEA Lysate Titer 5.1 x 10^10
Pitt Freezer Box# 1
Pitt Freezer Box Grid# 3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload