Mycobacterium phage Chewbacca
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Detailed Information for Phage Chewbacca
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMegan Kays and Rodney Tan
Year Found2017
Location FoundLas Vegas, NV USA
Finding InstitutionUniversity of Nevada Las Vegas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates36.233013 N, 115.302754 W Map
Discovery NotesCollected from private home at Latitude 36.109452 Longitude -115.143600 at a depth of 6". The soil was moist, sandy, and filled with debris. It was collected from a semi-shady location under a 9 year old pomegranate tree that had been fertilized with organic homemade compost 3 months prior. Ambient temperature was 94 degrees Fahrenheit
Naming NotesBecause Chewbacca is awesome and has a giant crossbow.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 12, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3156
Genome length (bp)43575
Character of genome ends3' Sticky Overhang
Overhang Length13 bases
Overhang SequenceCCCGCCGCAATGG
GC Content66.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterN
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 5/15/2018)
Plaque NotesA lysogen that is consistently .4 mm in diameter, extremely turbid with a clearly defined halo on the exterior of plaque, with or without a bulls-eye in center.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.33 x 10^-10
Pitt Freezer Box# 52
Pitt Freezer Box Grid# C11
Available Files
Plaque PictureDownload
EM PictureDownload