Mycobacterium phage Colt
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Detailed Information for Phage Colt
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHaley Mulder and James Gonzalez
Year Found2015
Location FoundCorpus Christi, TX USA
Finding InstitutionDel Mar College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates27.69 N, 97.3784 W Map
Discovery NotesSoil sample was taken in a horse pasture near a small pond on August 31, 2015 at 9:05 AM. Sample was taken 1 1/2 inches under the surface. Sample is a gray clay with small amount of gravel in the soil. Soil contains some algae and has a moist composition. Weather condition was overcast with a slight wind at 1mph, 85% humidity, 0% precipitation, and it was 80 degrees F outside.
Naming NotesPhage was named after my miniature horse named Colt.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 28, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage907
Genome length (bp)158508
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

TinyC (None)

Trilla23 (None)

Turret (None)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Breeniome (C1)

Bxz1 (C1)

Cali (C1)

Catera (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Delilah (C1)

Derek (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

FoxtrotP1 (C1)

FrayBell (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

HarrySpotter (C1)

HyRo (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LRRHood (C1)

Lukilu (C1)

Lysocool (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Napoleon13 (C1)

Nappy (C1)

Nicolasphaqe (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phox (C1)

Pier (C1)

Pio (C1)

Pleione (C1)

QBert (C1)

Rizal (C1)

Roots515 (C1)

SandySandal (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zeenon (C1)

ZygoTaiga (C1)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Plaque NotesSubsequent to serial dilution, spot assays and streak tests, the putative plaque which was isolated and further refined through MTL & HTL protocols produced lytic plaques which were approximately 0.5 mm in diameter, consistent in morphology throughout the area of the agar plate and also consistent in opacity. The area of the individual plaques did not exceed the aforementioned measurement, and were robust in quantity.
MorphotypeMyoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Colt_1

Colt_2

Colt_3

Colt_4

Colt_5

Colt_6

Colt_7

Colt_8

Colt_9

Colt_10

Colt_11

Colt_12

Colt_13

Colt_14

Colt_15

Colt_16

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Colt_18

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Colt_20

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Colt_22

Colt_23

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Colt_27

Colt_28

Colt_29

Colt_30

Colt_31

Colt_32

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Colt_34

Colt_35

Colt_36

Colt_37

Colt_38

Colt_39

Colt_40

Colt_41

Colt_42

Colt_43

Colt_44

Colt_45

Colt_46

Colt_47

Colt_48

Colt_49

Colt_50

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Colt_52

Colt_53

Colt_54

Colt_55

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Colt_70

Colt_71

Colt_72

Colt_73

Colt_74

Colt_78

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Colt_101

Colt_102

Colt_103

Colt_104

Colt_105

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Colt_122

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Colt_125

Colt_126

Colt_127

Colt_128

Colt_129

Colt_130

Colt_131

Colt_132

Colt_133

Colt_134

Colt_135

Colt_136

Colt_137

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Colt_139

Colt_140

Colt_141

Colt_142

Colt_143

Colt_144

Colt_145

Colt_146

Colt_150

Colt_151

Colt_153

Colt_155

Colt_160

Colt_166

Colt_170

Colt_171

Colt_177

Colt_184

Colt_185

Colt_186

Colt_187

Colt_188

Colt_189

Colt_190

Colt_191

Colt_192

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Colt_194

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Colt_198

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Colt_202

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Colt_206

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Colt_211

Colt_212

Colt_213

Colt_214

Colt_215

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Colt_217

Colt_218

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Colt_220

Colt_221

Colt_224

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Colt_226

Colt_227

Colt_228

Colt_229

Colt_230

Colt_231

Colt_232

Colt_233

Colt_234

Colt_235

Colt_236

Colt_237

Colt_238

Colt_239

Colt_240

Colt_241

Colt_242

Colt_243

Colt_244

Colt_245

Colt_246

Colt_247

Colt_248

Colt_249

Colt_250

Colt_251

Colt_252

Colt_253

Colt_254

Colt_255

Colt_256

Colt_257

Colt_258

Colt_259

Colt_260

Colt_261

Colt_262

Colt_263

Colt_264

Colt_265

Colt_267

Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload