| Detailed Information for Phage Crossroads |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Crossroads2K |
| Found By | Wayne Sera |
| Year Found | 2011 |
| Location Found | North Huntingdon, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.326475 N, 79.677855 W Map |
| Discovery Notes | *** Crossroads2K became the 2000th phage added to the database on 11.29.11! *** |
| Naming Notes | "Crossroads" is from a classic Robert Johnson song. It is the 2,000th phage. |
| Mutants |
| Mutants of this Phage |
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 25, 2012 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 131 |
| Sanger Finishing Reactions | 14 |
| Genome length (bp) | 76129 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 58.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Original enriched sample yield 1 plaque, approximately 1 mm in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 133 |
| Number of tRNAs | 12 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KF024731 |
| Refseq Number | NC_022071 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
E8 |
| Available Files |
| EM Picture | Download |
| Final DNAMaster File | Download |