Mycobacterium phage Daegal
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Detailed Information for Phage Daegal
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLeani Oosthuizen
Year Found2017
Location FoundAuckland, New Zealand
Finding InstitutionMassey University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates36.734 S, 174.701 E Map
Discovery NotesFound at the Massey University Community Garden Box, in wet, soft soil under straw. Adopted from another student in the program (Natasha Elliston-Boyes).
Temperature on day of discovery was 21 degrees Celsius.
Naming NotesDaegal is an Anglo-Saxon name that means "Dweller by the dark stream." This was apt as the phage was found in a garden near a forest stream. It also refers to this phage 'hiding' until it was isolated by phage-hunters.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 27, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3483
Genome length (bp)54858
Character of genome ends3' Sticky Overhang
Overhang Length14 bases
Overhang SequenceTAAGCCGCGCGGTA
GC Content67.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterQ
Subcluster--
Other Cluster Members
Plaque NotesPlaques are very consistent in size and morphology. They are approximately 0.75 mm in diameter. Plaques are uniformly and completely clear in the centre with a circular in shape and clearly defined edges.

Some evidence of lysogenic mesa was seen when very high titres (10^9) were spotted on bacterial lawn. The mesa in the centre of the clearance was not very opaque but definitely more cloudy than the usual completely clear lytic plaques.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload