Gordonia phage Demosthenes
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Detailed Information for Phage Demosthenes
Discovery Information
Isolation HostGordonia terrae 3612
Found ByPatrick Rimple
Year Found2015
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.436419 N, 79.949316 W Map
Discovery NotesThis phage was found on the middle of a path down in panther hollow that crosses between the railroad tracks and the pond. It had rained the day before and was around 80 degrees F that day. The soil was the wet top layer that people had stepped on.
Naming NotesDemosthenes is the name a character in the Ender's Game series adopts so that she may write deep philosophical papers. I really liked that so I wanted to name one of my phages after her, and in so I also honor the ancient Greek statesman of the same name who tried to keep Athens free form Macedonia.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 3, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1146
Genome length (bp)74073
Character of genome endsDirect Terminal Repeat
End Determination NotesBuildup of reads flanking region of increased coverage.
GC Content59.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS4
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesPlaques are clear and pinprick size to 1mm in size.
Number of Genes95
Has been Phamerated?Yes
Gene List

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Demosthenes_1

Demosthenes_2

Demosthenes_3
(DNA binding domain protein)

Demosthenes_4

Demosthenes_5

Demosthenes_6

Demosthenes_7

Demosthenes_8
(DNA primase)

Demosthenes_9
(terminase small subunit)

Demosthenes_10
(terminase large subunit)

Demosthenes_11
(portal protein)

Demosthenes_12
(tail fiber protein)

Demosthenes_13

Demosthenes_14
(capsid maturation protease)

Demosthenes_15
(scaffolding protein)

Demosthenes_16
(major capsid protein)

Demosthenes_17
(head-to-tail connector protein)

Demosthenes_18
(head-to-tail connector protein)

Demosthenes_19
(head-to-tail connector protein)

Demosthenes_20
(head-to-tail connector protein)

Demosthenes_21
(head-to-tail connector protein)

Demosthenes_22
(head-to-tail connector protein)

Demosthenes_23
(major tail protein)

Demosthenes_24
(tail assembly chaperone)

Demosthenes_25
(tail assembly chaperone)

Demosthenes_26
(tape measure protein)

Demosthenes_27
(minor tail protein)

Demosthenes_28
(minor tail protein)

Demosthenes_29
(minor tail protein)

Demosthenes_30

Demosthenes_31
(membrane protein)

Demosthenes_32

Demosthenes_33
(lysin A)

Demosthenes_34
(lysin A)

Demosthenes_35
(membrane protein)

Demosthenes_36
(membrane protein)

Demosthenes_37
(membrane protein)

Demosthenes_38
(RecB-like exonuclease)

Demosthenes_39
(WhiB family transcription factor)

Demosthenes_40

Demosthenes_41

Demosthenes_42
(DNA methylase)

Demosthenes_43
(lysin B)

Demosthenes_44
(nucleotide pyrophosphohydrolase)

Demosthenes_45

Demosthenes_46
(RusA-like ribonuclease)

Demosthenes_47

Demosthenes_48

Demosthenes_49
(DNA polymerase III beta subunit)

Demosthenes_50

Demosthenes_51
(exonuclease)

Demosthenes_52
(HTH DNA binding domain protein)

Demosthenes_53

Demosthenes_54

Demosthenes_55
(DNA helicase)

Demosthenes_56

Demosthenes_57

Demosthenes_58

Demosthenes_59

Demosthenes_60

Demosthenes_61

Demosthenes_62

Demosthenes_63

Demosthenes_64

Demosthenes_65

Demosthenes_66

Demosthenes_67

Demosthenes_68

Demosthenes_69

Demosthenes_70

Demosthenes_71

Demosthenes_72
(HNH endonuclease)

Demosthenes_73

Demosthenes_74

Demosthenes_75

Demosthenes_76
(cobalamin biosynthesis protein)

Demosthenes_77

Demosthenes_78

Demosthenes_79
(AAA ATPase)

Demosthenes_80

Demosthenes_81

Demosthenes_82

Demosthenes_83

Demosthenes_84

Demosthenes_85

Demosthenes_86

Demosthenes_87

Demosthenes_88

Demosthenes_89

Demosthenes_90

Demosthenes_91

Demosthenes_92

Demosthenes_93

Demosthenes_94

Demosthenes_95

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU998242
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 4
Pitt Freezer Box Grid# G6
Available Files
Final DNAMaster FileDownload