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Mycobacterium phage Derek
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Detailed Information for Phage Derek
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBarbara Tsaousis
Year Found2015
Location FoundBethlehem, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature-1°C
GPS Coordinates40.6065 N, 75.372273 W Map
Discovery NotesDerek was discovered on Lehigh University's campus. The soil sample was taken from approximately 2 inches beneath the surface.
Naming NotesNamed after the fictional character Derek Shepherd, who was a neurosurgeon on ABC's Grey's Anatomy.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 5, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage423
Genome length (bp)156199
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Other Cluster Members

Click to View

Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

TinyC (None)

Trilla23 (None)

Turret (None)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Breeniome (C1)

Bxz1 (C1)

Cali (C1)

Catera (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Delilah (C1)

Derek (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

FoxtrotP1 (C1)

FrayBell (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

HarrySpotter (C1)

HyRo (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LRRHood (C1)

Lukilu (C1)

Lysocool (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Napoleon13 (C1)

Nappy (C1)

Nicolasphaqe (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phox (C1)

Pier (C1)

Pio (C1)

Pleione (C1)

QBert (C1)

Rizal (C1)

Roots515 (C1)

SandySandal (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zeenon (C1)

ZygoTaiga (C1)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Plaque NotesPlaques were originally very small (approx. 1mm) and turbid when phages were left to infect M. smegmatis at 37 degrees Celsius, but it was discovered that they developed an opaque, shadowy region surrounding each plaque upon spending excessive time at cold temperatures (4 degrees Celsius). This shadowy region was distinct from the plaques and was confirmed by a repeated trial. It was also confirmed that said regions were not present after undergoing regular incubation at 37 degrees. Picture provided is of a plate that was not subjected to colder temperatures.
MorphotypeMyoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

Derek_1

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Derek_154

Derek_155

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Derek_264

Derek_266

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload