Mycobacterium phage ElephantFly
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Detailed Information for Phage ElephantFly
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEmily Xu
Year Found2016
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.98722 N, 86.45222 E Map
Discovery NotesMy phage was found in between rocks and under some dead leaves on campus. It had recently stopped raining so the dirt was moist and cool. The sample was collected with some bio debris.
Naming NotesNamed after the song Elephant Fly from the Disney movie Dumbo, "But I be done seen 'bout ev'rything
When I see a ElephantFly"
Sequencing Information
Sequencing Complete?No
Sequencing FacilityPittsburgh Bacteriophage Institute
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaque are moderately sized, about one millimeter in diameter. The plaques were also turbid (not super turbid but not close to clear either) as seen in the attached image.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2016WEKUelephantflyEX
SEA Lysate Titer 7.3 x 10^8 pfu/mL
Date of SEA Lysate Titering Nov 22, 2016
Pitt Freezer Box# 37
Pitt Freezer Box Grid# B12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload