| Detailed Information for Phage Enoki | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Former names | Enoki5 |
| Found By | Becky Phan |
| Year Found | 2014 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.965 N, 118.4425 W Map |
| Discovery Notes | Isolated from forehead microcomedones. Phage isolation and genome annotation performed by Hannah Bucko, Andrea Mayorga, Dallas Mould, and Becky Phan (UCLA). DNA preparation prepared by Krisanavane Reddi (UCLA). Assembly and quality control of annotation results performed by Jordan Moberg Parker and Dallas Mould (UCLA). |
| Naming Notes | Named after mushroom with tiny head and long stem. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 24, 2015 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 135 |
| Genome length (bp) | 29347 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 54.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Bull’s eye (plaques with clear perimeters and turbid centers); average size 6 mm in diameter (range of 3-7 mm) |
| Morphotype | Siphoviridae |
| Number of Genes | 45 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewEnoki_1 Enoki_2 Enoki_3 Enoki_4 Enoki_5 Enoki_6 Enoki_7 Enoki_8 Enoki_9 Enoki_10 Enoki_11 Enoki_12 Enoki_13 Enoki_14 Enoki_15 Enoki_16 Enoki_17 Enoki_18 Enoki_19 Enoki_20 Enoki_21 Enoki_22 Enoki_23 Enoki_24 Enoki_25 Enoki_26 Enoki_27 Enoki_28 Enoki_29 Enoki_30 Enoki_31 Enoki_32 Enoki_33 Enoki_34 Enoki_35 Enoki_36 Enoki_37 Enoki_38 Enoki_39 Enoki_40 Enoki_41 Enoki_42 Enoki_43 Enoki_44 Enoki_45 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR347353 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2014UCLAenokiBP |
| SEA Lysate Titer | 9.3x10^8 pfu/ml |
| Date of SEA Lysate Titering | May 26, 2014 |
| Pitt Freezer Box# | 23 |
| Pitt Freezer Box Grid# | D5 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |