| Detailed Information for Phage Evanesce |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Meghan Keleher |
| Year Found | 2012 |
| Location Found | Hartford, CT USA |
| Finding Institution | Trinity College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.75 N, 72.69 W Map |
| Discovery Notes | Soil collected in front of the Trinity College library on a patch of mostly dead grass. The soil was light to medium colors brown on surface but much darker once dug a little further down. Soil sample taken near a storing drain and sewer. No sprinklers nearby. |
| Naming Notes | Evanesce was chosen as it is a synonym for disappearing. When the EM picture was being taken of my phage, the tails would begin to disappear before the picture could be taken. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Ion Torrent Sequencing |
| Approximate Shotgun Coverage | 1040 |
| Sanger Finishing Reactions | 10 |
| Genome length (bp) | 53746 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 14 bases |
| Overhang Sequence | TAAGCCGCGCGGTA |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | Q |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Phage is clear with a slight bull's eye. Faint turbid "halo". Mean diameter 24 hours post-infection was 0.8mm. 48 hours post- infection the phage grew in size to about 2.4mm and had a dark bull's eye in the middle with a lighter dark ring around the bull's eye and then a clear halo surrounding that. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT454972 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
39 |
| Pitt Freezer Box Grid# |
71 |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |
