Mycobacterium phage Evanesce
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Detailed Information for Phage Evanesce
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMeghan Keleher
Year Found2012
Location FoundHartford, CT United States
Finding InstitutionTrinity College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.75 N, 72.69 W Map
Discovery NotesSoil collected in front of the Trinity College library on a patch of mostly dead grass. The soil was light to medium colors brown on surface but much darker once dug a little further down. Soil sample taken near a storing drain and sewer. No sprinklers nearby.
Naming NotesEvanesce was chosen as it is a synonym for disappearing. When the EM picture was being taken of my phage, the tails would begin to disappear before the picture could be taken.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage1040
Sanger Finishing Reactions10
Genome length (bp)53746
Character of genome ends3' Sticky Overhang
Overhang Length14 bp
Overhang SequenceTAAGCCGCGCGGTA
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterQ
Subcluster--
Other Cluster Members
Plaque NotesPhage is clear with a slight bull's eye. Faint turbid "halo". Mean diameter 24 hours post-infection was 0.8mm. 48 hours post- infection the phage grew in size to about 2.4mm and had a dark bull's eye in the middle with a lighter dark ring around the bull's eye and then a clear halo surrounding that.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

Evanesce_1
(terminase small subunit)

Evanesce_2

Evanesce_3

Evanesce_4

Evanesce_5
(terminase large subunit)

Evanesce_6
(portal)

Evanesce_7
(capsid maturation protease)

Evanesce_8
(scaffolding)

Evanesce_9
(capsid)

Evanesce_10
(head-to-tail connector)

Evanesce_11
(head-to-tail connector)

Evanesce_12
(head-to-tail connector)

Evanesce_13
(head-to-tail connector)

Evanesce_14
(head-to-tail connector)

Evanesce_15
(major tail subunit)

Evanesce_16
(tail assembly chaperone)

Evanesce_17
(tail assembly chaperone)

Evanesce_18

Evanesce_19

Evanesce_20
(tapemeasure)

Evanesce_21
(minor tail protein)

Evanesce_22
(minor tail protein)

Evanesce_23
(minor tail protein)

Evanesce_24
(minor tail protein)

Evanesce_25
(minor tail protein)

Evanesce_26

Evanesce_27
(minor tail protein)

Evanesce_28

Evanesce_29
(integrase (Y-int))

Evanesce_30
(excise)

Evanesce_31
(lysin A)

Evanesce_32
(lysin B)

Evanesce_33
(holin)

Evanesce_34

Evanesce_35

Evanesce_36

Evanesce_37

Evanesce_38

Evanesce_39

Evanesce_40
(HTH DNA binding)

Evanesce_41

Evanesce_42

Evanesce_43
(DNA binding)

Evanesce_44

Evanesce_45

Evanesce_46

Evanesce_47

Evanesce_48
(immunity repressor)

Evanesce_49
(Cro-like)

Evanesce_50

Evanesce_51

Evanesce_52
(HTH DNA binding)

Evanesce_53

Evanesce_54
(RecE-like)

Evanesce_55
(RecT-like)

Evanesce_56

Evanesce_57

Evanesce_58

Evanesce_59

Evanesce_60

Evanesce_61

Evanesce_62

Evanesce_63
(exonuclease)

Evanesce_64

Evanesce_65

Evanesce_66
(DNA methylase)

Evanesce_67

Evanesce_68

Evanesce_69

Evanesce_70

Evanesce_71

Evanesce_72

Evanesce_73
(RuvC-like Holliday junction resolvase)

Evanesce_74
(WhiB-like transcriptional regulator)

Evanesce_75

Evanesce_76

Evanesce_77

Evanesce_78

Evanesce_79

Evanesce_80

Evanesce_81

Evanesce_82
(HNH endonuclease)

Evanesce_83

Evanesce_84

Evanesce_85

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT454972
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Restriction Digest PictureDownload
EM PictureDownload