| Detailed Information for Phage Filch |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Philip Versluis |
| Year Found | 2014 |
| Location Found | IL |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.266319 N, 88.024447 W Map |
| Naming Notes | The name Filch was given because none of the environmental samples that had been personally collected produced an isolated phage. An extra plate from a colleague that yielded plaques was "filched" with permission and purification proceeded.
Filch is also the nickname given to a past dog. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 4, 2020 |
| Sequencing Facility | Hope College |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 4944 |
| Genome length (bp) | 75692 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCTTGTCA |
| GC Content | 62.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | E |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Filch is a temperate phage, although the plaque morphology produced is mostly clear; Filch is capable of producing a stable lysogen. |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | Filch was isolated, and purified at 32 degrees Celsius. After 24 hours Filch exhibited clear plaques with diameter just below one millimeter. Filch continued to grow until 72 hours when it attained a plaque 3-4 millimeters in diameter.
After 48 hours of incubation, a turbid half ring appeared around the clear plaque.
Filch was incubated at 42 and 32 degrees Celsius. Plaques grew at 32 as well as 42, though Filch may be sensitive to the higher temperature. |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ622163 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
7 |
| Pitt Freezer Box Grid# |
E12 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
