Mycobacterium phage Filodough
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| Detailed Information for Phage Filodough | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Julia Melehani |
| Year Found | 2017 |
| Location Found | La Cañada Flintridge, CA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 10°C |
| GPS Coordinates | 34.222546 N, 118.196362 W Map |
| Discovery Notes | It was taken outside on a hill-like surface. The environment of which the enriched sample was extracted, was raining for five days straight. The soil was shipped across the country possibly with the change of elevation and temperature having an effect. The soil amount taken was about a handful with rocks and pebbles along with the enriched sample. The soil was damp and cold throughout the extraction of the enriched sample. |
| Naming Notes | It is a personal name for me since my dog's name is Filo and he was my first pet I've ever had growing up. I decided to combine his name with a specific type of dough I use to make Baklava and other middle eastern pastry desserts, Phyllo dough. I decided to use my dog's name, Filo, to replace the spelling for Phyllo, in Phyllo dough, since they are both pronounced the same. It's an inside reference for those who know my dog and for those that know my love for baking especially using this dough making my favorite Middle Eastern desserts. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Throughout phage purification and plating procedures, the plaques were particularly medium in size, about 2 millimeters, and were clear with a turbid ring around the plaque. For the purification rounds, the plaque appeared in the span of 24 hours. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 6e10 pfu/mL |
| Date of SEA Lysate Titering | Mar 7, 2017 |
| Pitt Freezer Box# | 51 |
| Pitt Freezer Box Grid# | C5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
