Gordonia phage Flakey
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Detailed Information for Phage Flakey
Discovery Information
Isolation HostGordonia terrae 3612
Found ByKatarina Gauger and Morley Struss
Year Found2016
Location FoundWinneconne, WI United States
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature29°C
GPS Coordinates44.118564 N, 88.722831 W Map
Discovery NotesFound in a garden near a lake. It had rained the day before. The collection day was sunny, but not humid. There was a cool breeze.
Naming NotesWe came up with the name Flakey, since we had a difficult time getting plaques. Flakey is a slang term for unreliable which describes our journey working with this phage. Flakey also means eccentric, which fits our phage perfectly with its small plaques and long tail.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 6, 2017
Genome length (bp)76889
Character of genome endsUnknown
Direct Terminal Repeat Length191 bp
GC Content58.9%
Sequencing NotesThis genome was sequenced as part of a DOGEMS sample.
Fasta file available?Yes: Download fasta file
Characterization
ClusterCS
SubclusterCS2
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaques are 1 mm. They are turbid, uniform, and do not have a halo.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Flakey_1

Flakey_2

Flakey_3

Flakey_4

Flakey_5

Flakey_6

Flakey_7

Flakey_8

Flakey_9

Flakey_10
(terminase)

Flakey_11
(portal protein)

Flakey_12
(hydrolase domain)

Flakey_13

Flakey_14
(capsid maturation protease)

Flakey_15
(scaffolding protein)

Flakey_16
(major capsid protein)

Flakey_17

Flakey_18

Flakey_19
(head-to-tail connector protein complex)

Flakey_20

Flakey_21

Flakey_22

Flakey_23
(major tail protein)

Flakey_25
(tail assembly chaperone)

Flakey_26
(tail assembly chaperone)

Flakey_27

Flakey_28
(minor tail protein)

Flakey_29
(minor tail protein)

Flakey_30
(minor tail protein)

Flakey_31
(membrane protein)

Flakey_32
(membrane protein)

Flakey_33
(membrane protein)

Flakey_34
(lysin A, N-acetyl-beta-D-muramidase domain)

Flakey_35
(lysin A, peptidase domain)

Flakey_36
(membrane protein)

Flakey_37
(membrane protein)

Flakey_38
(membrane protein)

Flakey_39
(membrane protein)

Flakey_40
(endonuclease/helicase)

Flakey_41

Flakey_42

Flakey_43

Flakey_44

Flakey_45

Flakey_46
(DNA methylase)

Flakey_47

Flakey_48

Flakey_49
(lysin B)

Flakey_50
(nucleotide pyrophosphohydrolase)

Flakey_51

Flakey_52

Flakey_53

Flakey_54
(DNA polymerase III sliding clamp (beta))

Flakey_55
(exonuclease)

Flakey_56
(HTH DNA-binding protein)

Flakey_57

Flakey_58
(DNA helicase)

Flakey_59

Flakey_60
(zinc finger DNA binding protein)

Flakey_61

Flakey_62

Flakey_63

Flakey_64

Flakey_65

Flakey_66

Flakey_67

Flakey_68

Flakey_69

Flakey_70

Flakey_71

Flakey_72

Flakey_73

Flakey_74

Flakey_75

Flakey_76

Flakey_77

Flakey_78

Flakey_79

Flakey_80

Flakey_81

Flakey_82

Flakey_83
(HNH endonuclease)

Flakey_84

Flakey_85

Flakey_86

Flakey_87

Flakey_88

Flakey_89
(cobalamin biosynthesis protein)

Flakey_90
(AAA-ATPase)

Flakey_91

Flakey_92

Flakey_93

Flakey_94

Flakey_95

Flakey_96

Flakey_97

Flakey_98

Flakey_99

Flakey_100

Flakey_101

Flakey_102

Flakey_103

Flakey_104

Flakey_105

Flakey_106

Flakey_107

Flakey_108

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG770211
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 8.42 X 10^7 pfu/mL
Date of SEA Lysate Titering Oct 17, 2016
Pitt Freezer Box# 37
Pitt Freezer Box Grid# F1
Available Files
EM PictureDownload
GenBank File for PhameratorDownload