| Detailed Information for Phage Flare16 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Sherafghan Khan |
| Year Found | 2015 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.9842 N, 86.4549 W Map |
| Discovery Notes | Discovered on Western Kentucky University's campus from the base of a tree at College Heights. The Air temperature was 87F (30.5 C). The depth the soil was collected was at approximately 4cm. The moisture of the soil was dry, but not brittle. The date the soil sample was collected was 8/31/15. The soil sample was collected at about 3:00 pm. |
| Naming Notes | This phage is named Flare16. The reason for this name is because the tail of the phage flares out at the end. The 16 comes from the age I was when I discovered this phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 23, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Approximate Shotgun Coverage | 567 |
| Genome length (bp) | 52858 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGTCGGTTA |
| GC Content | 63.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | A |
| Subcluster | A2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | This phage is a temperate bacteriophage, therefore the plaque morphology is turbid instead of clear. |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques formed by Flare16 were turbid. Flare16 plaques retained the same turbidity and size (~3mm)
after 24 hours of incubation at 37C compared to 48 hours of incubation at the same temperature. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH825701 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2015WEKUFlare16SK |
| SEA Lysate Titer |
1.82 X 10^8 pfu/ml |
| Date of SEA Lysate Titering |
Oct 16, 2015 |
| Pitt Freezer Box# |
27 |
| Pitt Freezer Box Grid# |
E5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
