Microbacterium phage Fork
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Detailed Information for Phage Fork
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByKiley Jones & Olivia Heil
Year Found2017
Location FoundRiver Falls, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates44.850833 N, 92.619166 W Map
Discovery NotesThe soil was found near the South Fork River right on the river bank under a bridge. Almost near 6th St in River Falls, WI. It was a moist, wet condition when found. The weather was a temperature of 72 degrees and cloudy/rainy.
Naming NotesWe named this phage Fork from the river it was found in, "South Fork".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 10, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2399
Genome length (bp)62090
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length3099 bp
GC Content61.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterED
SubclusterED2
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesFork was a visible plaque that was just a small hole on the agar plate. When first picking plaques there were three different shapes to differ from. Fork was picked from the soil extract plate and when the process was redone there was a uniform supply of visible plaques on the plate that was later named Fork.
Has been Phamerated?Yes
Gene List

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Fork_1

Fork_2
(helix-turn-helix DNA binding domain protein)

Fork_3

Fork_4

Fork_5

Fork_6

Fork_7
(membrane protein)

Fork_8

Fork_9

Fork_10

Fork_11
(DNA binding protein)

Fork_12

Fork_13

Fork_14

Fork_15

Fork_16

Fork_17

Fork_18

Fork_19

Fork_20

Fork_21

Fork_22

Fork_23

Fork_26

Fork_27

Fork_28

Fork_29

Fork_30

Fork_31
(NrdH-like glutaredoxin)

Fork_32
(metallophosphoesterase)

Fork_33

Fork_34
(HNH endonuclease)

Fork_35
(terminase)

Fork_36
(portal protein)

Fork_37
(capsid maturation protease)

Fork_38

Fork_39
(major capsid protein)

Fork_40

Fork_41

Fork_42

Fork_43
(hydrolase)

Fork_44

Fork_45

Fork_46

Fork_47

Fork_48

Fork_49

Fork_50
(tail assembly chaperone)

Fork_51
(tail assembly chaperone)

Fork_52
(tape measure protein)

Fork_53

Fork_54
(minor tail protein)

Fork_55

Fork_56

Fork_57

Fork_58
(lysin A)

Fork_59
(membrane protein)

Fork_60
(membrane protein)

Fork_61
(resolvase)

Fork_62

Fork_63

Fork_64

Fork_65

Fork_66
(DNA primase)

Fork_67

Fork_68
(membrane protein)

Fork_69

Fork_70
(DNA helicase)

Fork_71

Fork_72

Fork_73
(exonuclease)

Fork_74

Fork_75

Fork_76
(RNA ligase)

Fork_77
(polynucleotide kinase)

Fork_78

Fork_79

Fork_80
(helicase)

Fork_81

Fork_82

Fork_83

Fork_84

Fork_85

Fork_86

Fork_87
(membrane protein)

Fork_88
(membrane protein)

Fork_89

Fork_90

Fork_91
(membrane protein)

Fork_92

Fork_93

Fork_94

Fork_95

Fork_96

Fork_97

Fork_98
(helix-turn-helix DNA binding domain protein)

Fork_99

Fork_100

Fork_101

Fork_102

Fork_103

Fork_104

Fork_105

Fork_106
(membrane protein)

Fork_107

Fork_108

Fork_109

Fork_110

Fork_111

Fork_112

Fork_113

Fork_114
(helix-turn-helix DNA binding domain protein)

Fork_115

Fork_116

Fork_117

Fork_118

Fork_119
(membrane protein)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH371108
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 69
Pitt Freezer Box Grid# F11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload