Mycobacterium phage Graves
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| Detailed Information for Phage Graves | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Adriana Mirabelli |
| Year Found | 2017 |
| Location Found | Holland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | The environmental sample was collected on September 14th, 2017. It was 71 degrees F, mostly cloudy, and 71% humidity. The sample was found approximately 2-3 inches under mulch beneath a petunia plant. Said bush was in a flower bed near a road on the Hope College campus. The soil collected had a slightly sandy texture and was relatively damp upon collection. |
| Naming Notes | This phage was discovered on Hope College's campus under a petunia bush. Said bush was located in front of Graves Hall, one of the oldest buildings on campus, and was thus named after this building. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | J |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | “Graves” appears to have a circular shape and very defined edges, a clear center- characteristic of a lytic phage- and turbid edges to form a halo. “Graves” often only demonstrates a halo morphology in larger plaques and when it is incubated for longer than 48 hours. It has demonstrated characteristics of a slow growing phage and grows best at approximately 72-96 hours at 37 degrees Celsius (3-4 days). Incubation that falls within this time frame yields halos and turbid edges; any less incubation time than this yields a smaller morphology of plaques that lack these turbid edges or halos. The original plaque picked was .5cm in diameter (including the halo) with a .2cm cleared center. Between all the plaque assay tests, plaques ranged from .5cm (largest plaque diameter including halo morphology) down to .1 cm (smallest plaque diameter lacking halo morphology). |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.0x10^11 |
| Pitt Freezer Box# | 50 |
| Pitt Freezer Box Grid# | D4 |