Gordonia phage Guacamole
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Detailed Information for Phage Guacamole
Discovery Information
Isolation HostGordonia terrae 3612
Found ByRebecca Rohac
Year Found2015
Location FoundPittsburgh , PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates40.445396 N, 79.957995 W Map
Discovery Notes-Weather conditions during collection: overcast but still humid, 85 degrees fahrenheit
-Weather conditions 24hrs prior to collection: less sun and more clouds, highs in the upper 80s and lows in the 70s
- Time of collection: 3:00pm, sun was at its peak
-Soil Conditions: warm, damp, area, with lots of leaves present
- Soil was transported in backpack for about 5-6 hours before being properly stored.
Found in lawn across from the Chevron Science Center, in the back lawn of the Space Research Coordination Center
Naming NotesThe name for this phage was inspired by Chipotle Mexican Grille's delicious guacamole.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 13, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage809
Genome length (bp)49894
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGCCGGTGA
GC Content67.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCV
Subcluster--
Other Cluster Members
Plaque NotesSmall circles with a clear center
Final annotation complete?Yes
Number of Genes78
Has been Phamerated?Yes
Gene List

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Guacamole_1
(Terminase, small subunit)

Guacamole_2
(Terminase, large subunit)

Guacamole_3
(Portal Protein)

Guacamole_4
(Capsid maturation protease)

Guacamole_5
(Scaffolding protein)

Guacamole_6
(Major capsid protein)

Guacamole_7
(Head-to-tail connector protein)

Guacamole_8
(Head-to-tail connector protein)

Guacamole_9
(Head-to-tail connector protein)

Guacamole_10

Guacamole_11
(Head-to-tail connector protein)

Guacamole_12
(Head-to-tail connector protein)

Guacamole_13
(Head-to-tail connector protein)

Guacamole_14
(Major tail protein)

Guacamole_15
(tail assembly chaperone)

Guacamole_16
(tail assembly chaperone)

Guacamole_17
(Tape Measure Protein)

Guacamole_18
(Minor tail protein)

Guacamole_19
(Minor tail protein)

Guacamole_20
(Lysin A, L-ala-D-Glu peptidase domain)

Guacamole_21
(Lysin A, glycosyl hydrolase domain)

Guacamole_22
(membrane protein)

Guacamole_23
(membrane protein)

Guacamole_24
(membrane protein)

Guacamole_25
(Lysin B)

Guacamole_26

Guacamole_27
(Minor tail protein)

Guacamole_28
(minor tail protein)

Guacamole_29

Guacamole_30
(minor tail protein)

Guacamole_31
(Acyltransferase)

Guacamole_32

Guacamole_33

Guacamole_34

Guacamole_35

Guacamole_36
(HTH DNA binding domain)

Guacamole_37

Guacamole_38
(Integrase, Y-int)

Guacamole_39

Guacamole_40
(Immunity repressor)

Guacamole_41
(Excise)

Guacamole_42

Guacamole_43

Guacamole_44

Guacamole_45

Guacamole_46

Guacamole_47

Guacamole_48
(Helix-turn-helix DNA binding domain)

Guacamole_49

Guacamole_50

Guacamole_51

Guacamole_52

Guacamole_53

Guacamole_54
(DnaQ Exonuclease)

Guacamole_55
(RecE exonuclease)

Guacamole_56
(RecT ssDNA binding protein)

Guacamole_57

Guacamole_58

Guacamole_59

Guacamole_60

Guacamole_61

Guacamole_62

Guacamole_63
(Nucleotidyl transferase)

Guacamole_64

Guacamole_65

Guacamole_66

Guacamole_67
(HNH Endonuclease)

Guacamole_68

Guacamole_69

Guacamole_70

Guacamole_71

Guacamole_72

Guacamole_73
(RuvC-like resolvase)

Guacamole_74

Guacamole_75

Guacamole_76

Guacamole_77

Guacamole_78
(HNH endonuclease)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU963259
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Final DNAMaster FileDownload