| Detailed Information for Phage HannahD | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Hannah Dunnous |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.2633 N, 79.5725 W Map |
| Discovery Notes | The bacteriophage was obtained from a flower bed outside Litchfield Tower C on The University of Pittsburgh campus in Pittsburgh. The soil collected in the sample was fine and dry, and some rocks and dried up roots were also present in the sample. It was 81 degrees Fahrenheit outside, and the humidity was 69%. My soil sample was then put into a conical tube with phage buffer and was put on the shaker for 2 days. The soil and phage buffer mixture was then filtered to isolate phage particles. The isolated phage particles were then plated on an agar plate with bacterial medium host Godonia terrae 3612. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 614 |
| Genome length (bp) | 46095 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGCCGCGGTA |
| GC Content | 66.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DB |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewHannahD_1 HannahD_2 HannahD_3 HannahD_4 HannahD_5 HannahD_6 HannahD_7 HannahD_8 HannahD_9 HannahD_10 HannahD_11 HannahD_12 HannahD_13 HannahD_14 HannahD_15 HannahD_16 HannahD_17 HannahD_18 HannahD_19 HannahD_20 HannahD_21 HannahD_22 HannahD_23 HannahD_24 HannahD_25 HannahD_26 HannahD_27 HannahD_28 HannahD_29 HannahD_30 HannahD_31 HannahD_32 HannahD_33 HannahD_34 HannahD_35 HannahD_36 HannahD_37 HannahD_38 HannahD_39 HannahD_40 HannahD_41 HannahD_42 HannahD_43 HannahD_44 HannahD_45 HannahD_46 HannahD_47 HannahD_48 HannahD_49 HannahD_50 HannahD_51 HannahD_52 HannahD_53 HannahD_54 HannahD_55 HannahD_56 HannahD_57 HannahD_58 HannahD_59 HannahD_60 HannahD_61 HannahD_62 HannahD_63 HannahD_64 HannahD_65 HannahD_66 HannahD_67 HannahD_68 HannahD_69 HannahD_70 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK878901 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 90 |
| Pitt Freezer Box Grid# | C4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
