Rhodococcus phage Harlequin

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Detailed Information for Phage Harlequin
Discovery Information
Isolation HostRhodococcus erythropolis RIA 643
Found ByTyler Jester, Austin Dicus, Mallory Crawford
Year Found2015
Location FoundMonroe, LA United States
Finding InstitutionUniversity of Louisiana at Monroe
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Naming NotesThe brother of this Phage was named "Jester" so we named this related Phage the female counterpart, Harlequin, for the female jesters of old.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 27, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3869
Genome length (bp)46383
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGTGAT
GC Content58.8%
Fasta file available?Available upon entering GPS coordinates
Characterization
ClusterCA
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaques formed by the Phage's infection are clear in nature and moderately sized plaques are found in the infected plates within 18 hours
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Harlequin_1

Harlequin_2

Harlequin_3

Harlequin_7
(lysin A)

Harlequin_8
(terminase)

Harlequin_9
(portal protein)

Harlequin_10
(capsid maturation protease)

Harlequin_11
(scaffolding protein)

Harlequin_12
(major capsid protein)

Harlequin_13
(head-to-tail connector)

Harlequin_14
(head-to-tail connector)

Harlequin_15
(head-to-tail connector)

Harlequin_16
(head-to-tail connector)

Harlequin_17
(head-to-tail connector)

Harlequin_18
(major tail protein)

Harlequin_19
(tail assembly chaperone)

Harlequin_20
(tail assembly chaperone)

Harlequin_21
(tape measure protein)

Harlequin_22
(minor tail protein)

Harlequin_23
(minor tail protein)

Harlequin_24
(minor tail protein)

Harlequin_25
(holin)

Harlequin_26

Harlequin_27

Harlequin_28

Harlequin_29
(integrase)

Harlequin_30
(excise)

Harlequin_31

Harlequin_32
(deoxycytidylate deaminase)

Harlequin_33

Harlequin_34

Harlequin_35

Harlequin_36
(DNA polymerase)

Harlequin_37
(helix-turn-helix DNA binding domain protein)

Harlequin_38
(thymidylate synthase)

Harlequin_39

Harlequin_40
(ribonucleotide reductase)

Harlequin_41

Harlequin_42
(helix-turn-helix DNA binding domain protein)

Harlequin_43
(metallophosphoesterase)

Harlequin_44

Harlequin_45
(glutaredoxin)

Harlequin_46

Harlequin_47
(DNA primase)

Harlequin_48
(DNA primase)

Harlequin_49

Harlequin_50
(endonuclease VII)

Harlequin_51

Harlequin_52
(dsDNA helicase)

Harlequin_53

Harlequin_54

Harlequin_55
(exonuclease/helicase)

Harlequin_56

Harlequin_57
(helix-turn-helix DNA binding domain protein)

Harlequin_58

Harlequin_59

Harlequin_60

Harlequin_61

Harlequin_62

Harlequin_63

Harlequin_64
(ArdA-like antirestriction protein)

Harlequin_65

Harlequin_66

Harlequin_67

Harlequin_68

Harlequin_69

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX611788
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 22
Pitt Freezer Box Grid# H8
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload