| Detailed Information for Phage Herod |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Former names | JohntheBactist |
| Found By | Luke Livingston and Riley Barnes |
| Year Found | 2017 |
| Location Found | Arkadelphia, AR USA |
| Finding Institution | Ouachita Baptist University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.1265 N, 93.048953 W Map |
| Discovery Notes | It was found in a mound of sand and silt on the bank of the Ouachita River on the campus of Ouachita Baptist University. |
| Naming Notes | This phage is named in honor of the first phage that my lab partner and I discovered. The first phage did not produce measureable plaques, nor did it produced a large quantity of plaques. We named it John the Bactist because after we first performed a spot test to see if any of our soil samples contained bacteriophages, we were told that we didn't have a phage. The next day in class, much to our surprise, we found out that we did in fact have a phage because there was a spot on the petri dish. We considered ourselves blessed with a phage that we were never supposed to have, much like Elizabeth and Zechariah in the Bible. Since we were unable to continue to work with this bacteriophage, but the name is completely awesome, we decided to keep the name for our second (hopefully more successful) phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 26, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2037 |
| Genome length (bp) | 53826 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCTGGGGA |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Ouachita Baptist University |
| Annotation Status | In GenBank |
| Plaque Notes | slightly cloudy, produces different plaque sizes. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT889398 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.07x10^10 pfu/mL |
| Pitt Freezer Box# |
48 |
| Pitt Freezer Box Grid# |
F1 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
