| Detailed Information for Phage HumptyDumpty |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Jessica Mastrando |
| Year Found | 2015 |
| Location Found | Radnor, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.056944 N, 75.375833 W Map |
| Discovery Notes | The soil sample was taken from next to a tree where a rat hole was in the Dixon parking lot at Cabrini College. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 970 |
| Genome length (bp) | 69978 |
| Character of genome ends | Circularly Permuted |
| GC Content | 61.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AR |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Nature of the phage consists of plaques that are average in size, clear and are round. Humpty Dumpty also produces a consistent web pattern at T-6 with 0mM, 5mM, 25mM and 50mM CaCl2 top agar and an overnight incubation at both 30 degrees Celsius and room temperature. Humpty Dumpty also produces a consistent web pattern at T-6 with 0mM, 5mM, 25mM, and 50mM MgS04 top agar and overnight incubation at both 30 degree Celsius and room temperature. |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX855962 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
Cabrini College |
| SEA Lysate Titer |
2.15x10^8 pfu/mL |
| Date of SEA Lysate Titering |
Jul 30, 2015 |
| Pitt Freezer Box# |
149 |
| Pitt Freezer Box Grid# |
C5 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
