Mycobacterium phage HyRo
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Detailed Information for Phage HyRo
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHylton Rodel
Year Found2014
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.811771 S, 30.916203 E Map
Discovery NotesSoil Sample obtained from very dry, dusty soil located in the middle of a dirt foot path, likely being formed due to high traffic of dogs on the property. Sample obtained in winter (particularly dry season at isolation locale). Lysate pure and in third round of purification.
Naming NotesFirst two letters of the first and last names of the discoverer
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 18, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1140
Genome length (bp)153714
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

TinyC (None)

Trilla23 (None)

Turret (None)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Breeniome (C1)

Bxz1 (C1)

Cali (C1)

Catera (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Delilah (C1)

Derek (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

FoxtrotP1 (C1)

FrayBell (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

HarrySpotter (C1)

HyRo (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LRRHood (C1)

Lukilu (C1)

Lysocool (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Napoleon13 (C1)

Nappy (C1)

Nicolasphaqe (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rizal (C1)

Roots515 (C1)

SandySandal (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zeenon (C1)

ZygoTaiga (C1)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Plaque NotesPlaques are circular, clear and approximately 1 mm in diameter at 24h of incubation, growing only slightly larger upon further incubation, reaching approximately 1.75 - 2mm after 48h incubation. Plaque picture was taken after 24h incubation.
MorphotypeMyoviridae
Final annotation complete?Yes
Number of Genes224
Number of tRNAs26
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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HyRo_1

HyRo_2

HyRo_3
(Nucleotidyltransferase)

HyRo_4

HyRo_5

HyRo_6

HyRo_7

HyRo_8

HyRo_9

HyRo_10

HyRo_11

HyRo_12

HyRo_13

HyRo_14

HyRo_15

HyRo_16

HyRo_17

HyRo_18

HyRo_19

HyRo_20

HyRo_21

HyRo_22

HyRo_23

HyRo_24
(HNH Endonuclease)

HyRo_25

HyRo_26

HyRo_27

HyRo_28
(Membrane protein Band7-like protein)

HyRo_29

HyRo_30

HyRo_31

HyRo_32

HyRo_33

HyRo_34

HyRo_35

HyRo_36

HyRo_37

HyRo_38

HyRo_39

HyRo_40

HyRo_41

HyRo_42

HyRo_43

HyRo_44

HyRo_45

HyRo_46

HyRo_47

HyRo_48

HyRo_49

HyRo_50

HyRo_51
(Putative DNA binding protein)

HyRo_52

HyRo_53

HyRo_54

HyRo_55

HyRo_56

HyRo_57

HyRo_58

HyRo_59

HyRo_60

HyRo_61

HyRo_62

HyRo_63

HyRo_64

HyRo_65

HyRo_66

HyRo_67

HyRo_68

HyRo_69

HyRo_70

HyRo_71

HyRo_72

HyRo_73

HyRo_74

HyRo_75

HyRo_76
(ParB domain-like protein)

HyRo_80

HyRo_81

HyRo_82

HyRo_83

HyRo_84

HyRo_85
(ThyX Thymidylate synthase)

HyRo_86

HyRo_87

HyRo_88
(LysM domain protein)

HyRo_89

HyRo_90

HyRo_91
(Major Capsid Subunit)

HyRo_92
(Zinc finger DNA binding protein)

HyRo_93

HyRo_94

HyRo_95

HyRo_96

HyRo_97

HyRo_98

HyRo_99

HyRo_100

HyRo_101

HyRo_102

HyRo_103

HyRo_104

HyRo_105
(Putative head decoration protein)

HyRo_106

HyRo_107

HyRo_108

HyRo_109

HyRo_110

HyRo_111

HyRo_112

HyRo_113

HyRo_114

HyRo_115

HyRo_116

HyRo_117

HyRo_118

HyRo_119

HyRo_120

HyRo_121
(Tail assembly chaperone)

HyRo_122
(Tail assembly chaperone)

HyRo_123
(Tape measure protein)

HyRo_124

HyRo_125

HyRo_126

HyRo_127

HyRo_128

HyRo_129

HyRo_130

HyRo_131

HyRo_132

HyRo_133

HyRo_134

HyRo_135

HyRo_136

HyRo_137

HyRo_138

HyRo_139

HyRo_140

HyRo_141

HyRo_142

HyRo_143

HyRo_146

HyRo_147
(peptidyl tRNA hydrolase)

HyRo_153

HyRo_161
(HNH domain protein.)

HyRo_163
(D-ala-D-ala carboxypeptidase)

HyRo_164

HyRo_174

HyRo_175

HyRo_176
(DNA helicase)

HyRo_177

HyRo_178

HyRo_179

HyRo_180

HyRo_181

HyRo_182

HyRo_183

HyRo_184

HyRo_185

HyRo_186
(Aminotransferase family protein)

HyRo_187

HyRo_188

HyRo_189

HyRo_190
(DnaC protein)

HyRo_191
(DnaB helicase)

HyRo_192

HyRo_193
(DNA primase)

HyRo_194

HyRo_195

HyRo_196

HyRo_197
(DNA Pol III alpha)

HyRo_198
(RF1 peptide chain release factor)

HyRo_199
(RecA-like protein)

HyRo_200

HyRo_201

HyRo_202
(Cas4-like exonuclease)

HyRo_203
(RusA-like protein)

HyRo_204

HyRo_205

HyRo_206

HyRo_207

HyRo_208

HyRo_209

HyRo_210
(HNH Endonuclease)

HyRo_211

HyRo_212

HyRo_213

HyRo_214

HyRo_215

HyRo_216

HyRo_217

HyRo_218
(Ro RNA binding protein)

HyRo_219

HyRo_220

HyRo_221

HyRo_222

HyRo_223

HyRo_224

HyRo_225

HyRo_226

HyRo_227

HyRo_228
(Galactosyltransferase)

HyRo_229

HyRo_230
(Glycosyltransferase)

HyRo_231

HyRo_232

HyRo_233

HyRo_234

HyRo_235
(Lysin A)

HyRo_236
(Holin)

HyRo_237
(Lysin B)

HyRo_238
(terminase)

HyRo_239

HyRo_240

HyRo_241

HyRo_242

HyRo_243
(Polynucleotide kinase)

HyRo_244

HyRo_245
(Serine/Threonine kinase)

HyRo_246

HyRo_247

HyRo_248
(Adenylosuccinate synthetase PurA-like protein)

HyRo_249

HyRo_250

HyRo_251

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT281790
Refseq NumberNone yet
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload