Mycobacterium phage Katt
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Detailed Information for Phage Katt
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKatarzyna Waniolka
Year Found2018
Location FoundSaint Louis, MO United States
Finding InstitutionSt. Louis Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates38.564248 N, 90.492155 E Map
Discovery NotesMulched area near patio.
The location: mostly sunny.
Soil collected from 2-3 inches depth, below a bush covered with mulch. The soil was warmer at 9am and the temperature outdoors during collection of a sample was about 65°F. The sample collected contained phage Katt and phage Kasia (listed separately).
Soil sample was black, loose, contained little of moisture and some organic matter (roots).
Enriched fluid phage sample (with Kasia and Katt) produced a very dark orange pigment before the phages were isolated.
Naming NotesName came from first name - first 3 letters (Kat +t, and "t" stands for "team" =Katt).
Phage Katt was discovered by Katarzyna Waniolka, who worked on two phages (Phage Kasia and Katt) until begining of purification, after which she continued working on phage Kasia and phage Katt was given up for adoption to a student, Bob Bullock, who worked on purification experiments for Katt up to DNA extraction procedures. Also, Daniel H., joined Bob in the last amplification experiments up to DNA extraction procedures. Big gratitude to Bob and Daniel for their work.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotation StatusNot sequenced
Plaque NotesThe plaques formed were very small of very similar sizes and shapes: round and usually less than 1-2 mm depending on the number of phage particles used.

Phage Katt has long tail (291 nm on average) with 65 nm width of capsid and 10 nm width of tail.
The tail end had rather bulky appearance and the tail segments were visible with few areas along the tail being slightly wider.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
EM PictureDownload