Mycobacterium phage Katt
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|Detailed Information for Phage Katt|
|Isolation Host||Mycobacterium smegmatis mc²155|
|Found By||Katarzyna Waniolka|
|Location Found||Saint Louis, MO United States|
|Finding Institution||St. Louis Community College|
|Program||Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science|
|From enriched soil sample?||Yes|
|GPS Coordinates||38.564248 N, 90.492155 E Map|
|Discovery Notes||Mulched area near patio.|
The location: mostly sunny.
Soil collected from 2-3 inches depth, below a bush covered with mulch. The soil was warmer at 9am and the temperature outdoors during collection of a sample was about 65°F. The sample collected contained phage Katt and phage Kasia (listed separately).
Soil sample was black, loose, contained little of moisture and some organic matter (roots).
Enriched fluid phage sample (with Kasia and Katt) produced a very dark orange pigment before the phages were isolated.
|Naming Notes||Name came from first name - first 3 letters (Kat +t, and "t" stands for "team" =Katt). |
Phage Katt was discovered by Katarzyna Waniolka, who worked on two phages (Phage Kasia and Katt) until begining of purification, after which she continued working on phage Kasia and phage Katt was given up for adoption to a student, Bob Bullock, who worked on purification experiments for Katt up to DNA extraction procedures. Also, Daniel H., joined Bob in the last amplification experiments up to DNA extraction procedures. Big gratitude to Bob and Daniel for their work.
|Genome length (bp)||Unknown|
|Character of genome ends||Unknown|
|Fasta file available?||No|
|Annotation Status||Not sequenced|
|Plaque Notes||The plaques formed were very small of very similar sizes and shapes: round and usually less than 1-2 mm depending on the number of phage particles used.|
Phage Katt has long tail (291 nm on average) with 65 nm width of capsid and 10 nm width of tail.
The tail end had rather bulky appearance and the tail segments were visible with few areas along the tail being slightly wider.
|Has been Phamerated?||No|
|Uploaded to GenBank?||No|
|GenBank Accession||None yet|
|Refseq Number||None yet|
|Archiving status||Not in Pitt Archives|