| Detailed Information for Phage Keiki | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Found By | Kathleen Del Rosario |
| Year Found | 2013 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.065 N, 118.4425 W Map |
| Discovery Notes | Isolated from forehead microcomedones. Phage isolation and genome annotation performed by Jackie Aganon, Sandhya Ashokkumar, Joshua Chiou, and Kathleen Del Rosario (UCLA). DNA preparation performed by Krisanavane Reddi (UCLA). Assembly performed by William Villella (UCLA) and confirmed by Charles Bowman (University of Pittsburgh). Quality control of annotation results performed by Jordan Moberg Parker and Dallas Mould (UCLA). |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 26, 2015 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 136 |
| Genome length (bp) | 29339 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 54.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | clear, circular plaques; approximately 8.0mm, ranging from 5.0-12.0mm |
| Morphotype | Siphoviridae |
| Number of Genes | 45 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewKeiki_1 Keiki_2 Keiki_3 Keiki_4 Keiki_5 Keiki_6 Keiki_7 Keiki_8 Keiki_9 Keiki_10 Keiki_11 Keiki_12 Keiki_13 Keiki_14 Keiki_15 Keiki_16 Keiki_17 Keiki_18 Keiki_19 Keiki_20 Keiki_21 Keiki_22 Keiki_23 Keiki_24 Keiki_25 Keiki_26 Keiki_27 Keiki_28 Keiki_29 Keiki_30 Keiki_31 Keiki_32 Keiki_33 Keiki_34 Keiki_35 Keiki_36 Keiki_37 Keiki_38 Keiki_39 Keiki_40 Keiki_41 Keiki_42 Keiki_43 Keiki_44 Keiki_45 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR337649 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2013UCLAkeikiKD |
| SEA Lysate Titer | 5.0x10^9 pfu/mL |
| Date of SEA Lysate Titering | Nov 15, 2013 |
| Pitt Freezer Box# | 23 |
| Pitt Freezer Box Grid# | C3 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
