Microbacterium phage Knox
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Detailed Information for Phage Knox
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByAudrey Jonas
Year Found2016
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.446418 N, 79.954514 W Map
Discovery NotesSoil was found outside the Wyndham University Center in a well-kept flower bed. Temperature was about 26 degrees Celsius and it appeared to be well watered.
Naming NotesKnox is the middle name of the eleventh president, James Polk
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 16, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage873
Genome length (bp)41797
Character of genome endsCircularly Permuted
GC Content63.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEA
SubclusterEA1
Cluster Life CycleLytic
Lysogeny NotesGrowth of mesas and lysogeny efficiency using phage-seeded plates was attempted with this phage by Audrey Jonas. This phage does not make a lysogen.
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesPlaques are about 0.5 mm in diameter and clear with halos after incubation at 30 degrees Celsius for 18-24 hours. Plaques increase in size and become more visible after incubation at room temp for 6-12 hours following initial incubation
MorphotypeSiphoviridae
Number of Genes63
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG839021
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 5
Pitt Freezer Box Grid# B12
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload