Mycobacterium phage Laeta
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| Detailed Information for Phage Laeta | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Viribus |
| Found By | Sarah Olen |
| Year Found | 2017 |
| Location Found | Lancaster, PA USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 32°C |
| GPS Coordinates | 40.016389 N, 76.249722 W Map |
| Discovery Notes | "Laeta" was found in August 23, 2017, at 10:42 A.M. The sample site is a wood-chipped path with moist soil, many roots, and is usually shaded. The top 3.5 inches were collected. A Xeno Lysogen test was conducted on which "Laeta" grew, meaning it is not a N Cluster phage. A temperature sensitivity test was also carried out at a temperature of 42 degrees Celsius. At this temperature "Laeta" grew (as well as at 32 degrees Celsius). A time of Infection test was conducted as well, but there was no difference in quality or quantity of plaques. |
| Naming Notes | "Laeta" means "happy" in Latin. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques are clear with turbid edges usually ranging from 2-3mm in diameter. The morphology is density dependent, meaning at lower dilutions, the plaques are small and more squished. The Digest photo does not show cuts on HaeIII, but there are cuts farther down. l know this from a previous attempt where I let the electrophoresis run for longer. The phage's genomic DNA concentration is 419 ng/µL. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 50 |
| Pitt Freezer Box Grid# | D8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
