Mycobacterium phage LilTurb
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Detailed Information for Phage LilTurb
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHannah Watkins
Year Found2015
Location FoundDublin, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates36.70497 N, 88.81633 W Map
Discovery NotesAt the EM, either my titer was too low, or my phage just are not that present in the samples; the most I got in a picture was 3, but only one time. The ten plate lysate titer was 1.57x10^9
Naming NotesWhen at the EM, the professor helping thought it would be cute to name them the "LilTurds" since they are very turbid and hard to see, and have been a pain all semester. I chose "LilTurb" as a play off of LilTurds since they are TURBid plaques.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 1, 2016
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2613
Genome length (bp)53153
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGTCGGTTA
GC Content63.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterA
SubclusterA2
Cluster Life CycleTemperate
Lysogeny NotesThe plaques very medium sized and EXTREMELY turbid.
Other Cluster Members

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Afua (None)

Ajaxious (None)

Anonia (None)

BaxterTD (None)

Blazer (None)

BlueWhiteSuper (None)

BrownWilse (None)

BuddyNugget (None)

BuzzKiehl (None)

Chara (None)

Clarabelle (None)

Cornelius42 (None)

Cujo (None)

Dannah (None)

Drogon (None)

EGMO (None)

Fido (None)

Fieldrain (None)

FireFlyEcho (None)

FrannyJoe (None)

Freedman (None)

Geezy (None)

Gracie1 (None)

Hata (None)

HerbSipe (None)

HotBoxer (None)

ILoveMyInterns (None)

Interokirkus (None)

JeanneMarie (None)

JeckyllnHyde (None)

Julio (None)

Kilomegawatt (None)

Krzemiun (None)

LaChupacabra (None)

Lakemo2013 (None)

LilaGirl (None)

LilMac (None)

Lintoc (None)

LittleDude (None)

McKinnon95 (None)

MelBell (None)

MelJoe (None)

MortyC137 (None)

Nakoi (None)

Noodle17 (None)

Norris007 (None)

Ousia (None)

Pbnep65 (None)

PDonaz (None)

PerryPatch (None)

Phern (None)

Poofy (None)

Pwombat (None)

Raziel (None)

RedJewel (None)

Roetering (None)

Rover (None)

Scooter (None)

Serena (None)

Shayla (None)

Skpmule (None)

SmallSoldier (None)

SnapDragon (None)

SoletiStotinki (None)

SoupAstar (None)

Star (None)

Tala (None)

Terrance (None)

Terrence (None)

Tiff5 (None)

TizzleFlarp (None)

TosSolNia (None)

TreeHugger (None)

UMulchMonster (None)

Unsequencer (None)

Yost (None)

ZheroDRT (None)

Abrogate (A1)

Acme (A1)

Adahisdi (A1)

Aeneas (A1)

AFIS (A1)

Alberto11 (A1)

Alsfro (A1)

Alvin (A1)

Anglerfish (A1)

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Arlo (A1)

Atkinbua (A1)

Barriga (A1)

Beatrix (A1)

BeesKnees (A1)

Bethlehem (A1)

Big3 (A1)

Bigfoot (A1)

BigMamaLlama (A1)

BigMau (A1)

BigPaolini (A1)

BillKnuckles (A1)

Bircsak (A1)

Bison (A1)

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Bob3 (A1)

Bones (A1)

BPBiebs31 (A1)

Briton15 (A1)

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EasyLeonard (A1)

Edtherson (A1)

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Fajezeel (A1)

Fascinus (A1)

Fenn (A1)

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FlowerChild (A1)

Forsytheast (A1)

FourStarNutty (A1)

Francis47 (A1)

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Fushigi (A1)

GageAP (A1)

Gompeii16 (A1)

Graduation (A1)

Greg (A1)

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ILeeKay (A1)

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JC27 (A1)

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Littlefoot32 (A1)

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Mule (A1)

Museum (A1)

Naira (A1)

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PacerPaul (A1)

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Target (A1)

Tasp14 (A1)

TechBARfly (A1)

TheloniousMonk (A1)

Thor (A1)

Tommy (A1)

Traft412 (A1)

Treddle (A1)

Tripl3t (A1)

Trouble (A1)

Turj99 (A1)

U2 (A1)

Uffda (A1)

Venus (A1)

Violet (A1)

Watermelon (A1)

Wheeler (A1)

Widget (A1)

Wilkins (A1)

Zeeba (A1)

Zeeculate (A1)

Zelezo (A1)

Zephyr (A1)

Zeuska (A1)

Blitzkrieg (A10)

Chupacabra (A10)

ElSilvo (A10)

Goose (A10)

KittenMittens (A10)

Kristoff (A10)

Lindberg (A10)

OKCentral2016 (A10)

Rebeuca (A10)

RhynO (A10)

Severus (A10)

Trike (A10)

Twister (A10)

WalterMcMickey (A10)

Bachome (A11)

Bowtie (A11)

Bud (A11)

Ebony (A11)

Et2Brutus (A11)

Fibonacci (A11)

Flaverint (A11)

Hutc2 (A11)

IshmaelIII (A11)

Jabith (A11)

Joselito (A11)

Lucivia (A11)

Mabel (A11)

Mulciber (A11)

Munch (A11)

Orange (A11)

Salz (A11)

Snape (A11)

DarthPhader (A12)

Pharaoh (A12)

Refuge (A12)

Steamy (A12)

Phlei (A13)

Jeeves (A14)

Luchador (A14)

Onism (A14)

Anon (A15)

JSwag (A15)

KatherineG (A15)

LastResort (A15)

Nedarya (A15)

Remus (A15)

Rosalind (A15)

ShayRa (A15)

Soups (A15)

Strosahl (A15)

Waits (A15)

EagleEye (A16)

40AC (A17)

MyraDee (A18)

Kimona (A19)

20ES (A2)

AbbyPaige (A2)

Acolyte (A2)

Adzzy (A2)

Agape74 (A2)

AnnaL29 (A2)

Anselm (A2)

Aquarose (A2)

ArcherNM (A2)

AsepTech (A2)

Asuzu (A2)

Bactobuster (A2)

Baehexic (A2)

BiancaTri92 (A2)

BobSwaget (A2)

Brooklyn (A2)

Buddy (A2)

Bugsy (A2)

Bulldog (A2)

Centaur (A2)

Changeling (A2)

Charity (A2)

Che12 (A2)

Cheriba (A2)

ChipMunk (A2)

ClumpyY (A2)

CRB1 (A2)

Crucio (A2)

Crush (A2)

D29 (A2)

Dalmatian (A2)

DBQu4n (A2)

Death (A2)

Drake55 (A2)

DudeLittle (A2)

Echild (A2)

ElFluff (A2)

Epoch (A2)

Equemioh13 (A2)

EvilGenius (A2)

Fameo (A2)

FiringLine (A2)

First (A2)

Flare16 (A2)

GaugeLDP (A2)

Georgie2 (A2)

Goethe (A2)

GoZags (A2)

GridersLegacy (A2)

Heffalump (A2)

IronMan (A2)

Iwokeuplikedis (A2)

Jaan (A2)

Jerm (A2)

Jinga3000 (A2)

JoshKayV (A2)

Journey13 (A2)

Jsquared (A2)

Kakar (A2)

Kalpine (A2)

Kempella (A2)

Kerberos (A2)

KingSt (A2)

Kupalo (A2)

L5 (A2)

Laderlappen (A2)

LadyBird (A2)

Larenn (A2)

Leogania (A2)

LilTurb (A2)

Lincaica11 (A2)

Lokk (A2)

Loser (A2)

Maduna (A2)

Malec (A2)

Masha1295 (A2)

Miko (A2)

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NothingSpecial (A2)

Odin (A2)

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Pim618 (A2)

Piro94 (A2)

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Power (A2)

Pukovnik (A2)

QueenB2 (A2)

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Rachaly (A2)

Radar (A2)

Rauana (A2)

RedRock (A2)

Retro23 (A2)

Scorpio7 (A2)

SemperFi (A2)

Serenity (A2)

Shipshape (A2)

SnapTap (A2)

StarStuff (A2)

Stumpy (A2)

Superchunk (A2)

SweetiePie (A2)

Tiberius20 (A2)

TipsytheTRex (A2)

Tomathan (A2)

Trixie (A2)

Turbido (A2)

Tyrone (A2)

Updawg (A2)

Whabigail7 (A2)

WideWale (A2)

Woozle13 (A2)

Yahighness (A2)

Zaaboot (A2)

Anthony (A20)

ACFishhook (A3)

Aglet (A3)

AgronaGT15 (A3)

Anubis (A3)

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AugsMagnumOpus (A3)

BabyRay (A3)

Beauxregard13 (A3)

BreSam8 (A3)

BuzzBuzz (A3)

Bxz2 (A3)

DaHudson (A3)

Daishi (A3)

Dieselweasel (A3)

Dumpling (A3)

EpicPhail (A3)

Farber (A3)

Fernando (A3)

FloMo (A3)

Fred313 (A3)

Gemma (A3)

GingkoMaracino (A3)

Gravata (A3)

Groupthink (A3)

Grum1 (A3)

GtownJaz (A3)

HelDan (A3)

Heliosoles (A3)

Hercules11 (A3)

Hookmount (A3)

Idleandcovert (A3)

Isca (A3)

JenCasNa (A3)

JeppNRM (A3)

JHC117 (A3)

Jobu08 (A3)

KADY (A3)

Kalb97 (A3)

Kalnoky (A3)

LadyPhaGa (A3)

Lambert1 (A3)

Lilith (A3)

Louie6 (A3)

LugYA (A3)

MadMarie (A3)

Mainiac (A3)

Malinsilva (A3)

Margo (A3)

Marie (A3)

Marius (A3)

MarQuardt (A3)

MatrixWest360 (A3)

Methuselah (A3)

Microwolf (A3)

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MoneyMay (A3)

MuchMore (A3)

Noella (A3)

Norbert (A3)

OlanP (A3)

Ollie (A3)

Ostojic (A3)

P28Green (A3)

Panamaxus (A3)

Penny1 (A3)

PGHhamlin (A3)

Phantastic (A3)

PhishRPhriends (A3)

Phoxy (A3)

Phranny (A3)

Pistachio (A3)

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Popcicle (A3)

PotatoSplit (A3)

Puppy (A3)

PurpleHaze (A3)

QuinnKiro (A3)

Reba (A3)

Roadbarry13 (A3)

Rockstar (A3)

Romeo (A3)

Sabia (A3)

Sabinator (A3)

SoilDragon (A3)

Soshari (A3)

Spike509 (A3)

Stagni (A3)

Stephen (A3)

StepMih (A3)

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Texage (A3)

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TNguyen7 (A3)

Todacoro (A3)

Veracruz (A3)

Vix (A3)

Watson (A3)

Wonder (A3)

Wooldri (A3)

Zetzy (A3)

AbbysRanger (A4)

Abdiel (A4)

Achebe (A4)

Albee (A4)

Annyong (A4)

Arturo (A4)

AvatarAhPeg (A4)

Avle17 (A4)

Baby16 (A4)

Backyardigan (A4)

Bacon (A4)

Badger (A4)

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BellusTerra (A4)

Blackmoor (A4)

Bombshell (A4)

Broseidon (A4)

Bruiser (A4)

BubbleTrouble (A4)

Bumblebee11 (A4)

Burger (A4)

Caelakin (A4)

Camperdownii (A4)

CentreCat (A4)

Cerulean (A4)

ChampagnePapi (A4)

Cici (A4)

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Citius (A4)

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DAgoa1 (A4)

Datway (A4)

DCNugh (A4)

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DirtyDunning (A4)

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Houdini22 (A4)

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Iracema64 (A4)

Jaykayelowell (A4)

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LittleB (A4)

LittleGuy (A4)

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Mazhar510 (A4)

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Melvin (A4)

Millski (A4)

Milton (A4)

Miramae (A4)

Morpher26 (A4)

Morrow (A4)

Mundrea (A4)

Nebs (A4)

Nemo27 (A4)

NorthStar (A4)

NotAPhaseMom (A4)

Nyxis (A4)

Obama12 (A4)

Ohfah (A4)

Palestino (A4)

Peaches (A4)

PeterPeter (A4)

Phacado (A4)

Phighter1804 (A4)

Phontbonne (A4)

Pipcraft (A4)

Relief (A4)

Romney (A4)

Roosevelt (A4)

Ruin (A4)

Sabertooth (A4)

Shaka (A4)

SheldonCooper (A4)

Skipitt (A4)

SophiesChoice (A4)

Stasia (A4)

Thanksgivukkah (A4)

TimCurry (A4)

TinaFeyge (A4)

Tinybot (A4)

TiroTheta9 (A4)

Tpap (A4)

TygerBlood (A4)

Wander (A4)

Wilbur (A4)

Wile (A4)

Wizard007 (A4)

Xena (A4)

AgentM (A5)

Airmid (A5)

Aragog (A5)

Archetta (A5)

Barbarella (A5)

Benedict (A5)

Bluefalcon (A5)

Chadwick (A5)

Conspiracy (A5)

Coog (A5)

Cuco (A5)

Dublin (A5)

Electri (A5)

ElTiger69 (A5)

ForGetIt (A5)

George (A5)

HuhtaEnerson15 (A5)

Jabiru (A5)

Jovo (A5)

Lev2 (A5)

LittleCherry (A5)

MarysWell (A5)

Midas2 (A5)

Naca (A5)

Phlorence (A5)

Scorpia (A5)

Swirley (A5)

Tarynearal (A5)

Theia (A5)

Tiger (A5)

UnionJack (A5)

WootieJr (A5)

Yolo (A5)

Artemis2UCLA (A6)

Blue7 (A6)

CloudWang3 (A6)

DaVinci (A6)

EricB (A6)

Gladiator (A6)

GreedyLawyer (A6)

Gruunaga (A6)

Hammer (A6)

Hexamo (A6)

Isiphiwo (A6)

Jeffabunny (A6)

JewelBug (A6)

Jordennis (A6)

Kazan (A6)

Koko (A6)

McFly (A6)

Paper (A6)

Pmask (A6)

Priamo (A6)

SuperAwesome (A6)

ToneTone (A6)

VohminGhazi (A6)

Wiks (A6)

WunderPhul (A6)

Yokurt (A6)

Zaka (A6)

Zulu (A6)

HINdeR (A7)

Sheen (A7)

Timshel (A7)

Astro (A8)

Cyrano (A8)

Dixon (A8)

Expelliarmus (A8)

Fredward (A8)

Kirpan (A8)

Machaera (A8)

Mitchell (A8)

NearlyHeadless (A8)

Roary (A8)

Saintus (A8)

Shresh (A8)

Smeadley (A8)

Stephig9 (A8)

Aliter (A9)

Alma (A9)

BogosyJay (A9)

Catalina (A9)

Charm (A9)

Conquerage (A9)

DreamTeam1 (A9)

Eidsmoe (A9)

Elephantoon (A9)

ExplosioNervosa (A9)

HortumSL17 (A9)

Keziacharles14 (A9)

Klaus (A9)

LoneWolf (A9)

Maminiaina (A9)

Myxus (A9)

Noelle (A9)

PackMan (A9)

Phaeder (A9)

Phonnegut (A9)

Pioneer (A9)

Priya (A9)

Qobbit (A9)

Rahalelujah (A9)

Scherzo (A9)

ShadowHunter (A9)

SheaKeira (A9)

Toaka (A9)

Tubs (A9)

Ugenie5 (A9)

Vanisoa (A9)

Yecey3 (A9)

Annotation StatusIn GenBank
Plaque NotesWhen working with this phage, the plaques were extremely turbid and very hard to see. When doing the purifications, it was very tricky to make sure that I got one single plaque since they were very hard to see and medium sized.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

LilTurb_1

LilTurb_2

LilTurb_3
(toxin, VIP2-like)

LilTurb_4

LilTurb_5
(HNH endonuclease)

LilTurb_6

LilTurb_7
(minor tail protein)

LilTurb_8

LilTurb_10

LilTurb_11
(lysin A)

LilTurb_12
(Holin)

LilTurb_13
(lysin B)

LilTurb_14
(Terminase)

LilTurb_15
(portal protein)

LilTurb_16
(Capsid maturation protease)

LilTurb_17
(scaffolding protein)

LilTurb_18
(major capsid protein)

LilTurb_19
(head-to-tail connector complex protein)

LilTurb_20
(head-to-tail connector complex protein)

LilTurb_21
(head-to-tail connector complex protein)

LilTurb_22
(head-to-tail connector complex protein)

LilTurb_23
(head-to-tail connector complex protein)

LilTurb_24
(head-to-tail connector complex protein)

LilTurb_25
(major tail protein)

LilTurb_26
(Tail assembly chaperone)

LilTurb_27
(tail assembly chaperone)

LilTurb_28
(tape measure protein)

LilTurb_29
(Minor Tail Protein)

LilTurb_30
(minor tail protein)

LilTurb_31
(Minor Tail Protein)

LilTurb_32
(Minor tail protein)

LilTurb_33
(Minor Tail Protein)

LilTurb_34
(integrase, (Y-int))

LilTurb_35
(membrane protein)

LilTurb_36

LilTurb_37
(excise)

LilTurb_38

LilTurb_39
(membrane protein)

LilTurb_40

LilTurb_41

LilTurb_42

LilTurb_43

LilTurb_44

LilTurb_45
(DNA polymerase I)

LilTurb_46

LilTurb_47

LilTurb_48
(helix-turn-helix DNA binding domain)

LilTurb_49

LilTurb_50
(ThyX-like thymidylate synthase)

LilTurb_51

LilTurb_52
(Ribonucleotide Reductase)

LilTurb_53

LilTurb_54

LilTurb_55
(helix-turn-helix DNA binding domain)

LilTurb_56

LilTurb_57
(MRE11 double-strand break endo/exonuclease)

LilTurb_58

LilTurb_59
(NrdH-like Glutaredoxin)

LilTurb_60
(DNA Primase)

LilTurb_61
(DNA Primase)

LilTurb_62

LilTurb_63
(endonuclease VII)

LilTurb_64
(membrane protein)

LilTurb_65
(esterase)

LilTurb_66
(helix-turn-helix DNA binding domain)

LilTurb_67

LilTurb_68

LilTurb_69
(DNAB-like dsDNA helicase)

LilTurb_70

LilTurb_71

LilTurb_72

LilTurb_73

LilTurb_74
(RecB-like Exonuclease)

LilTurb_75

LilTurb_76
(Immunity Repressor)

LilTurb_77

LilTurb_78

LilTurb_79

LilTurb_80

LilTurb_81

LilTurb_82

LilTurb_83

LilTurb_84
(membrane protein)

LilTurb_85

LilTurb_86

LilTurb_87
(membrane protein)

LilTurb_88

LilTurb_89

LilTurb_90

LilTurb_91

LilTurb_92

LilTurb_93

LilTurb_94

LilTurb_95

LilTurb_96

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH825704
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.57 x 10^9
Date of SEA Lysate Titering Nov 3, 2015
Pitt Freezer Box# 27
Pitt Freezer Box Grid# F3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload